Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?

J. P. J. Saeij; Rene Josephus Maria Stet; B. de Vries; W. B. Van Muiswinkel; G. F. Wiegertjes

doi:10.1016/S0145-305X(02)00064-2

Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?

J. P. J. Saeij, Rene Josephus Maria Stet, B. de Vries, W. B. Van Muiswinkel, G. F. Wiegertjes

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145 Citations (Scopus)

Abstract

Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	29-41
Number of pages	12
Journal	Developmental and Comparative Immunology
Volume	27
DOIs	https://doi.org/10.1016/S0145-305X(02)00064-2
Publication status	Published - 2003

Keywords

TNF
parasite
fish
cytokine
nitric oxide
Kinetoplastida
infection
phagocyte
TUMOR-NECROSIS-FACTOR
ALPHA MESSENGER-RNA
COMMON CARP
CYPRINUS-CARPIO
NITRIC-OXIDE
3'-UNTRANSLATED REGION
COMPLEMENT C3
EXPRESSION
CLONING
PENTOXIFYLLINE

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10.1016/S0145-305X(02)00064-2

Cite this

@article{d35dd7461f454b2ba8f13dfcb8b7053a,

title = "Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?",

abstract = "Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.",

keywords = "TNF, parasite, fish, cytokine, nitric oxide, Kinetoplastida, infection, phagocyte, TUMOR-NECROSIS-FACTOR, ALPHA MESSENGER-RNA, COMMON CARP, CYPRINUS-CARPIO, NITRIC-OXIDE, 3'-UNTRANSLATED REGION, COMPLEMENT C3, EXPRESSION, CLONING, PENTOXIFYLLINE",

author = "Saeij, {J. P. J.} and Stet, {Rene Josephus Maria} and {de Vries}, B. and {Van Muiswinkel}, {W. B.} and Wiegertjes, {G. F.}",

year = "2003",

doi = "10.1016/S0145-305X(02)00064-2",

language = "English",

volume = "27",

pages = "29--41",

journal = "Developmental and Comparative Immunology",