Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?

J. P. J. Saeij, Rene Josephus Maria Stet, B. de Vries, W. B. Van Muiswinkel, G. F. Wiegertjes

    Research output: Contribution to journalArticle

    125 Citations (Scopus)

    Abstract

    Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)29-41
    Number of pages12
    JournalDevelopmental and Comparative Immunology
    Volume27
    DOIs
    Publication statusPublished - 2003

    Keywords

    • TNF
    • parasite
    • fish
    • cytokine
    • nitric oxide
    • Kinetoplastida
    • infection
    • phagocyte
    • TUMOR-NECROSIS-FACTOR
    • ALPHA MESSENGER-RNA
    • COMMON CARP
    • CYPRINUS-CARPIO
    • NITRIC-OXIDE
    • 3'-UNTRANSLATED REGION
    • COMPLEMENT C3
    • EXPRESSION
    • CLONING
    • PENTOXIFYLLINE

    Cite this

    Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance? / Saeij, J. P. J.; Stet, Rene Josephus Maria; de Vries, B.; Van Muiswinkel, W. B.; Wiegertjes, G. F.

    In: Developmental and Comparative Immunology, Vol. 27, 2003, p. 29-41.

    Research output: Contribution to journalArticle

    Saeij, J. P. J. ; Stet, Rene Josephus Maria ; de Vries, B. ; Van Muiswinkel, W. B. ; Wiegertjes, G. F. / Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?. In: Developmental and Comparative Immunology. 2003 ; Vol. 27. pp. 29-41.
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    abstract = "Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.",
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    AU - Saeij, J. P. J.

    AU - Stet, Rene Josephus Maria

    AU - de Vries, B.

    AU - Van Muiswinkel, W. B.

    AU - Wiegertjes, G. F.

    PY - 2003

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    AB - Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.

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    KW - parasite

    KW - fish

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    KW - nitric oxide

    KW - Kinetoplastida

    KW - infection

    KW - phagocyte

    KW - TUMOR-NECROSIS-FACTOR

    KW - ALPHA MESSENGER-RNA

    KW - COMMON CARP

    KW - CYPRINUS-CARPIO

    KW - NITRIC-OXIDE

    KW - 3'-UNTRANSLATED REGION

    KW - COMPLEMENT C3

    KW - EXPRESSION

    KW - CLONING

    KW - PENTOXIFYLLINE

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    JO - Developmental and Comparative Immunology

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