Molecular and functional characterization of carp TNF:a link between TNF polymorphism and trypanotolerance?

J. P. J. Saeij, Rene Josephus Maria Stet, B. de Vries, W. B. Van Muiswinkel, G. F. Wiegertjes

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    Abstract

    Two carp tumor necrosis factor alpha (TNFalpha) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3' UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFalpha in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFalpha-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFalpha in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found. (C) 2002 Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)29-41
    Number of pages12
    JournalDevelopmental and Comparative Immunology
    Volume27
    DOIs
    Publication statusPublished - 2003

    Keywords

    • TNF
    • parasite
    • fish
    • cytokine
    • nitric oxide
    • Kinetoplastida
    • infection
    • phagocyte
    • TUMOR-NECROSIS-FACTOR
    • ALPHA MESSENGER-RNA
    • COMMON CARP
    • CYPRINUS-CARPIO
    • NITRIC-OXIDE
    • 3'-UNTRANSLATED REGION
    • COMPLEMENT C3
    • EXPRESSION
    • CLONING
    • PENTOXIFYLLINE

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