Molecular approaches in the study of Biomphalaria glabrata - Schistosoma mansoni interactions: linkage analysis and gene expression profiling

C S Jones, A E Lockyer, D Rollinson, L R Noble

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Gene mapping and the generation of linkage groups are fundamental to an understanding of the organization and relationships of genes and marker sequences, providing a framework with which to investigate their association with traits of interest. The abundance of techniques available for generating polymorphic molecular markers, and recent advances in high throughput screening, have allowed the extension of map analysis to the tropical freshwater snail Biomphalaria glabrata, an important intermediate host for Schistosoma mansoni. Direct comparison of gene expression by differential display screening, without prior identification of candidate genes, can be combined with mapping to quantify the involvement of specific sequences in the schistosome resistance response, and other important host-parasite interactions. Here we discuss the application of current and emergent technologies to gene characterization and linkage analysis in snail-schistosome interactions. Preliminary results from the analysis of comparative gene expression in resistant and susceptible snails are also presented.

Original languageEnglish
Pages (from-to)181-196
Number of pages16
JournalParasitology
Volume123
Issue number07
DOIs
Publication statusPublished - Nov 2001

Fingerprint

Biomphalaria
Biomphalaria glabrata
Schistosoma mansoni
Snails
Gene Expression Profiling
linkage (genetics)
snails
Schistosoma
gene expression
screening
Gene Expression
Host-Parasite Interactions
genes
Chromosome Mapping
host-parasite relationships
Genetic Association Studies
intermediate hosts
Fresh Water
linkage groups
Genes

Keywords

  • Biomphalaria glabrata
  • Schistosoma mansoni
  • linkage analyses
  • comparative gene expression
  • representational difference analysis
  • single-nucleotide polymorphisms
  • intermediate snail host
  • arbitrarily primed PCR
  • fresh-water
  • sequence tags
  • human genome
  • microsatellite variation
  • mass-spectrometry
  • rare transcripts

Cite this

Molecular approaches in the study of Biomphalaria glabrata - Schistosoma mansoni interactions : linkage analysis and gene expression profiling. / Jones, C S ; Lockyer, A E ; Rollinson, D ; Noble, L R .

In: Parasitology, Vol. 123, No. 07, 11.2001, p. 181-196.

Research output: Contribution to journalArticle

@article{a87467d4f34b4ce38ef4f055b7be175e,
title = "Molecular approaches in the study of Biomphalaria glabrata - Schistosoma mansoni interactions: linkage analysis and gene expression profiling",
abstract = "Gene mapping and the generation of linkage groups are fundamental to an understanding of the organization and relationships of genes and marker sequences, providing a framework with which to investigate their association with traits of interest. The abundance of techniques available for generating polymorphic molecular markers, and recent advances in high throughput screening, have allowed the extension of map analysis to the tropical freshwater snail Biomphalaria glabrata, an important intermediate host for Schistosoma mansoni. Direct comparison of gene expression by differential display screening, without prior identification of candidate genes, can be combined with mapping to quantify the involvement of specific sequences in the schistosome resistance response, and other important host-parasite interactions. Here we discuss the application of current and emergent technologies to gene characterization and linkage analysis in snail-schistosome interactions. Preliminary results from the analysis of comparative gene expression in resistant and susceptible snails are also presented.",
keywords = "Biomphalaria glabrata, Schistosoma mansoni, linkage analyses, comparative gene expression, representational difference analysis, single-nucleotide polymorphisms, intermediate snail host, arbitrarily primed PCR, fresh-water, sequence tags, human genome, microsatellite variation, mass-spectrometry, rare transcripts",
author = "Jones, {C S} and Lockyer, {A E} and D Rollinson and Noble, {L R}",
year = "2001",
month = "11",
doi = "10.1017/S0031182001008174",
language = "English",
volume = "123",
pages = "181--196",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "07",

}

TY - JOUR

T1 - Molecular approaches in the study of Biomphalaria glabrata - Schistosoma mansoni interactions

T2 - linkage analysis and gene expression profiling

AU - Jones, C S

AU - Lockyer, A E

AU - Rollinson, D

AU - Noble, L R

PY - 2001/11

Y1 - 2001/11

N2 - Gene mapping and the generation of linkage groups are fundamental to an understanding of the organization and relationships of genes and marker sequences, providing a framework with which to investigate their association with traits of interest. The abundance of techniques available for generating polymorphic molecular markers, and recent advances in high throughput screening, have allowed the extension of map analysis to the tropical freshwater snail Biomphalaria glabrata, an important intermediate host for Schistosoma mansoni. Direct comparison of gene expression by differential display screening, without prior identification of candidate genes, can be combined with mapping to quantify the involvement of specific sequences in the schistosome resistance response, and other important host-parasite interactions. Here we discuss the application of current and emergent technologies to gene characterization and linkage analysis in snail-schistosome interactions. Preliminary results from the analysis of comparative gene expression in resistant and susceptible snails are also presented.

AB - Gene mapping and the generation of linkage groups are fundamental to an understanding of the organization and relationships of genes and marker sequences, providing a framework with which to investigate their association with traits of interest. The abundance of techniques available for generating polymorphic molecular markers, and recent advances in high throughput screening, have allowed the extension of map analysis to the tropical freshwater snail Biomphalaria glabrata, an important intermediate host for Schistosoma mansoni. Direct comparison of gene expression by differential display screening, without prior identification of candidate genes, can be combined with mapping to quantify the involvement of specific sequences in the schistosome resistance response, and other important host-parasite interactions. Here we discuss the application of current and emergent technologies to gene characterization and linkage analysis in snail-schistosome interactions. Preliminary results from the analysis of comparative gene expression in resistant and susceptible snails are also presented.

KW - Biomphalaria glabrata

KW - Schistosoma mansoni

KW - linkage analyses

KW - comparative gene expression

KW - representational difference analysis

KW - single-nucleotide polymorphisms

KW - intermediate snail host

KW - arbitrarily primed PCR

KW - fresh-water

KW - sequence tags

KW - human genome

KW - microsatellite variation

KW - mass-spectrometry

KW - rare transcripts

U2 - 10.1017/S0031182001008174

DO - 10.1017/S0031182001008174

M3 - Article

VL - 123

SP - 181

EP - 196

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 07

ER -