Molecular characterization of the HLA-DR2LUM haplotype

Neil Thomas Young; C Darke

doi:10.1111/j.1399-0039.1994.tb02292.x

Molecular characterization of the HLA-DR2LUM haplotype

Neil Thomas Young, C Darke

Applied Medicine

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.

Original language	English
Pages (from-to)	28-33
Number of pages	6
Journal	Tissue Antigens
Volume	43
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-0039.1994.tb02292.x
Publication status	Published - 1 Jan 1994

Keywords

Alleles
Base Sequence
Cell Line
DNA
DNA Probes
HLA-DR2 Antigen
Haplotypes
Humans
Lymphocytes
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

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10.1111/j.1399-0039.1994.tb02292.x

Cite this

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title = "Molecular characterization of the HLA-DR2LUM haplotype",

abstract = "Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational {"}hotspot{"} in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.",

keywords = "Alleles, Base Sequence, Cell Line, DNA, DNA Probes, HLA-DR2 Antigen, Haplotypes, Humans, Lymphocytes, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length",

author = "Young, {Neil Thomas} and C Darke",

year = "1994",

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doi = "10.1111/j.1399-0039.1994.tb02292.x",

language = "English",

volume = "43",

pages = "28--33",

journal = "Tissue Antigens",

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publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Molecular characterization of the HLA-DR2LUM haplotype

AU - Young, Neil Thomas

AU - Darke, C

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.

AB - Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.

KW - Alleles

KW - Base Sequence

KW - Cell Line

KW - DNA

KW - DNA Probes

KW - HLA-DR2 Antigen

KW - Haplotypes

KW - Humans

KW - Lymphocytes

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

U2 - 10.1111/j.1399-0039.1994.tb02292.x

DO - 10.1111/j.1399-0039.1994.tb02292.x

M3 - Article

C2 - 7912857

SN - 0001-2815

VL - 43

SP - 28

EP - 33

JO - Tissue Antigens

JF - Tissue Antigens

IS - 1

ER -

Molecular characterization of the HLA-DR2LUM haplotype

Abstract

Keywords

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