Molecular characterization of the HLA-DR2LUM haplotype

Neil Thomas Young, C Darke

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.
Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalTissue Antigens
Volume43
Issue number1
DOIs
Publication statusPublished - 1 Jan 1994

Fingerprint

Dichlororibofuranosylbenzimidazole
Haplotypes
Genes
HLA-DR alpha-Chains
HLA-DR1 Antigen
HLA-DR2 Antigen
Cells
Cell Line
MHC Class II Genes
Pseudogenes
Homologous Recombination
Restriction Fragment Length Polymorphisms
Immune Sera
Alleles
Polymerase Chain Reaction
HLA-DR2LUM
HLA-DR15 antigen

Keywords

  • Alleles
  • Base Sequence
  • Cell Line
  • DNA
  • DNA Probes
  • HLA-DR2 Antigen
  • Haplotypes
  • Humans
  • Lymphocytes
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length

Cite this

Molecular characterization of the HLA-DR2LUM haplotype. / Young, Neil Thomas; Darke, C.

In: Tissue Antigens, Vol. 43, No. 1, 01.01.1994, p. 28-33.

Research output: Contribution to journalArticle

Young, Neil Thomas ; Darke, C. / Molecular characterization of the HLA-DR2LUM haplotype. In: Tissue Antigens. 1994 ; Vol. 43, No. 1. pp. 28-33.
@article{98a6ca244da9412fba7350e7d8de30ae,
title = "Molecular characterization of the HLA-DR2LUM haplotype",
abstract = "Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational {"}hotspot{"} in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.",
keywords = "Alleles, Base Sequence, Cell Line, DNA, DNA Probes, HLA-DR2 Antigen, Haplotypes, Humans, Lymphocytes, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length",
author = "Young, {Neil Thomas} and C Darke",
year = "1994",
month = "1",
day = "1",
doi = "10.1111/j.1399-0039.1994.tb02292.x",
language = "English",
volume = "43",
pages = "28--33",
journal = "Tissue Antigens",
issn = "0001-2815",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Molecular characterization of the HLA-DR2LUM haplotype

AU - Young, Neil Thomas

AU - Darke, C

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.

AB - Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.

KW - Alleles

KW - Base Sequence

KW - Cell Line

KW - DNA

KW - DNA Probes

KW - HLA-DR2 Antigen

KW - Haplotypes

KW - Humans

KW - Lymphocytes

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

U2 - 10.1111/j.1399-0039.1994.tb02292.x

DO - 10.1111/j.1399-0039.1994.tb02292.x

M3 - Article

VL - 43

SP - 28

EP - 33

JO - Tissue Antigens

JF - Tissue Antigens

SN - 0001-2815

IS - 1

ER -