Molecular cloning of interleukin 1 beta from rainbow trout Oncorhynchus mykiss reveals no evidence of an ice cut site

J Zou, P S Grabowski, C Cunningham, C J Secombes

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188 Citations (Scopus)

Abstract

The complete coding sequence of rainbow trout IL-1 beta has been obtained. The gene contains a short 5' UTR (97 bp), a 780 bp open reading frame and a 466 6p 3' UTR, which includes a polyadenylation signal, 7 ATTTA motifs and an 18 bp poly ii tail. The predicted amino acid sequence (260 amino acids) contains 3 potential glycosylation sites, with a predicted molecular weight of 29 kDa, and show's between 49 and 56% amino acid similarity to mammalian IL-1 beta s and 57% similarity to carp IL-1 beta, Greatest homology was apparent within the secondary structure of the gene, with few of the amino acids known to bind to the IL-1 receptor being conserved. No ICE cut site was apparent but multiple alignment with mammalian sequences allowed a putative mature peptide of 166 amino acids to be identified, in which Ala(95) would be the amino terminus. Northern blot analysis showed that whilst no IL-1 beta expression was detectable in head kidney leukocytes immediately after isolation, expression could be induced by stimulation with LPS for 4 h in culture. Similarly, with isolated head kidney macrophages expression was significantly increased following stimulation with LPS. (C) 1999 Academic Press.

Original languageEnglish
Pages (from-to)552-560
Number of pages9
JournalCytokine
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 1999

Keywords

  • interleukin 1 beta
  • cDNA cloning
  • rainbow trout
  • cytokines
  • evolution
  • recombinant bovine interleukin-1-beta
  • differential evolution
  • xenopus embryo
  • IL-1 receptor
  • messenger-RNA
  • expression
  • gene
  • sequence
  • proteins

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