Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Rustam I Aminov; Alan W Walker; Sylvia H Duncan; Hermie J M Harmsen; Gjalt W Welling; Harry James Flint

doi:10.1128/AEM.00701-06

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Rustam I Aminov, Alan W Walker, Sylvia H Duncan, Hermie J M Harmsen, Gjalt W Welling, Harry James Flint

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

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Abstract

Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.

Original language	English
Pages (from-to)	6371-6376
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	9
DOIs	https://doi.org/10.1128/AEM.00701-06
Publication status	Published - Sept 2006

Keywords

Bacteria, Anaerobic
Base Sequence
Colony Count, Microbial
Digestive System
Eubacterium
Feces
Genes, Bacterial
Genetic Variation
Humans
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Oligonucleotide Probes
Phylogeny
RNA, Bacterial
RNA, Ribosomal, 16S
16s ribosomal RNA
butyrate-producing bacteria
human fecal samples
human feces
oligonucleotide probes
human colon
phylogenetic relationships
GEN-NOV
communities
accessibility

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AEM.00701-06

RoseburiaFinal published version, 194 KB

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Aminov, R. I., Walker, A. W., Duncan, S. H., Harmsen, H. J. M., Welling, G. W., & Flint, H. J. (2006). Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale. Applied and Environmental Microbiology, 72(9), 6371-6376. https://doi.org/10.1128/AEM.00701-06

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale. / Aminov, Rustam I; Walker, Alan W ; Duncan, Sylvia H et al.
In: Applied and Environmental Microbiology, Vol. 72, No. 9, 09.2006, p. 6371-6376.

Research output: Contribution to journal › Article › peer-review

Aminov, RI, Walker, AW , Duncan, SH, Harmsen, HJM, Welling, GW & Flint, HJ 2006, 'Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale', Applied and Environmental Microbiology, vol. 72, no. 9, pp. 6371-6376. https://doi.org/10.1128/AEM.00701-06

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abstract = "Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.",

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KW - Bacteria, Anaerobic

KW - Base Sequence

KW - Colony Count, Microbial

KW - Digestive System

KW - Eubacterium

KW - Feces

KW - Genes, Bacterial

KW - Genetic Variation

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Molecular Sequence Data

KW - Oligonucleotide Probes

KW - Phylogeny

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KW - RNA, Ribosomal, 16S

KW - 16s ribosomal RNA

KW - butyrate-producing bacteria

KW - human fecal samples

KW - human feces

KW - oligonucleotide probes

KW - human colon

KW - phylogenetic relationships

KW - GEN-NOV

KW - communities

KW - accessibility

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Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Abstract

Keywords

UN SDGs

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Cite this