Molecular Ecology Of Macrolide-Lincosamide-Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production

Satoshi Koike, Rustam Irekovitch Aminov, A. C. Yannarell, Holly D. Gans, Ivan G. Krapac, Joanne C. Chee-Sanford, Roderick I. Mackie

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

RNA methylase genes are common antibiotic resistance determinants for multiple drugs of the macrolide, lincosamide, and streptogramin B (MLSB) families. We used molecular methods to investigate the diversity, distribution, and abundance of MLSB methylases in waste lagoons and groundwater wells at two swine farms with a history of tylosin (a macrolide antibiotic structurally related to erythromycin) and tetracycline usage. Phylogenetic analysis guided primer design for quantification of MLSB resistance genes found in tylosin-producing Streptomyces (tlr(B), tlr(D)) and commensal/pathogenic bacteria (erm(A), erm(B), erm(C), erm(F), erm(G), erm(Q)). The near absence of tlr genes at these sites suggested a lack of native antibiotic-producing organisms. The gene combination erm(ABCF) was found in all lagoon samples analyzed. These four genes were also detected with high frequency in wells previously found to be contaminated by lagoon leakage. A weak correlation was found between the distribution of erm genes and previously reported patterns of tetracycline resistance determinants, suggesting that dissemination of these genes into the environment is not necessarily linked. Considerations of gene origins in history (i.e., phylogeny) and gene distributions in the landscape provide a useful "molecular ecology" framework for studying environmental spread of antibiotic resistance.

Original languageEnglish
Pages (from-to)487-498
Number of pages12
JournalMicrobial Ecology
Volume59
Issue number3
Early online date19 Nov 2009
DOIs
Publication statusPublished - Apr 2010

Keywords

  • tetracycline resistance genes
  • time PCR assays
  • antibiotic-resistance
  • production facilities
  • conferring resistance
  • tylosin
  • sequence
  • quantification
  • campylobacter
  • determinants

Cite this

Koike, S., Aminov, R. I., Yannarell, A. C., Gans, H. D., Krapac, I. G., Chee-Sanford, J. C., & Mackie, R. I. (2010). Molecular Ecology Of Macrolide-Lincosamide-Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production. Microbial Ecology, 59(3), 487-498. https://doi.org/10.1007/s00248-009-9610-0

Molecular Ecology Of Macrolide-Lincosamide-Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production. / Koike, Satoshi; Aminov, Rustam Irekovitch; Yannarell, A. C.; Gans, Holly D.; Krapac, Ivan G.; Chee-Sanford, Joanne C.; Mackie, Roderick I.

In: Microbial Ecology, Vol. 59, No. 3, 04.2010, p. 487-498.

Research output: Contribution to journalArticle

Koike, Satoshi ; Aminov, Rustam Irekovitch ; Yannarell, A. C. ; Gans, Holly D. ; Krapac, Ivan G. ; Chee-Sanford, Joanne C. ; Mackie, Roderick I. / Molecular Ecology Of Macrolide-Lincosamide-Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production. In: Microbial Ecology. 2010 ; Vol. 59, No. 3. pp. 487-498.
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