Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran

Nader Mosavari, Mohammad Mehdi Feizabadi, Mahmoud Jamshidian, Masoud Reza Seifiabadi Shahpouri, Ken J. Forbes, Reza Aref Pajoohi, Rouholah Keshavarz, Mohammad Mohammad Taheri, Keyvan Tadayon

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Abstract

Restriction fragment length polymorphism (RFLP) genotyping was employed to analyze the population genetics of Mycobacterium bovis in Iran. One hundred and twenty-three isolates collected from slaughtered tuberculosis-suspect cattle and one clinically asymptomatic buffalo were subjected to RFLP analysis with probes of the polymorphic GC-rich sequence (PGRS) and the direct repeat sequence (DR) using DNA digested with PvuII and AluI. All these methods detected a large homogeneous population in which only a few isolates had variant genotypes. Only AluI-based RFLPs of both the PGRS and DR sequences were able to clearly differentiate between BCG and field strains of M. bovis. As in previous reports, these findings seem to reflect a recent dispersal of one or a few strains in Iran following the substantial expansion of Holstein-Friesian cattle over the last few decades. (C) 2011 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalVeterinary Microbiology
Volume151
Issue number1-2
Early online date24 Feb 2011
DOIs
Publication statusPublished - 5 Jul 2011

Keywords

  • Mycobacterium bovis
  • RFLP
  • Buffalo
  • Cattle
  • Typing
  • Fragment-length-polymorphism
  • Tuberculosis complex
  • Infection

Cite this

Mosavari, N., Feizabadi, M. M., Jamshidian, M., Shahpouri, M. R. S., Forbes, K. J., Pajoohi, R. A., Keshavarz, R., Taheri, M. M., & Tadayon, K. (2011). Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran. Veterinary Microbiology, 151(1-2), 148-152. https://doi.org/10.1016/j.vetmic.2011.02.038