Molecular identification of schistosome intermediate hosts: case studies of Bulinus forskalii group species (Gastropoda : Planorbidae) from Central and East Africa

C S Jones, L R Noble, J Ouma, H C Kariuki, R Mimpfoundi, D S Brown, D Rollinson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

African freshwater snails of the genus Bullinus acr as intermediate hosts for schistosomes, trematode parasites responsible for medical and veterinary forms of schitosomiasis. The relationship between these snails and their parasites is an intricate one, with particular species of snail susceptible to infection only by certain species of schistosome. In common with other self-fertile hermaphrodite gastropods, Bulinus consists of a number of closely related species complexes with restricted gene flow between populations of each taxon. Consequently, despite their medical and veterinary importance as intermediate hosts, unambiguous identification and differentiation of planorbid snails such as these remains problematic, often confounding attempts to define the distribution and evolutionary relationships of conchologically similar taxa. Here we consider how morphological methods of discrimination can be used in conjunction with molecular based approaches to improve snail identification. thereby achieving a better understanding of the epidemiology of schistosomiasis. Data are presented from Central and East. African taxa which illustrate how PCR-bared methods have begun to be used in combination with traditional analyses in an integrated approach to characterize the genus Bulinus, specifically the B. forskalii species group. particular emphasis is given to the analyses of Random Amplified Polymorphic DNA (RAPDs) and die mitochondrial gene cytochrome oxidase I (COI). (C) 1999 The Linnean Society of London.

Original languageEnglish
Pages (from-to)215-240
Number of pages26
JournalBiological Journal of the Linnean Society
Volume68
Publication statusPublished - 1999

Keywords

  • mitochondrial DNA
  • cytochrome oxidase subunit I
  • COI
  • Randomly Amplified Polymorphic DNA
  • RAPDs
  • arbitrary primers
  • phylogenetics
  • morphology
  • morphometrics
  • POLYMORPHIC DNA RAPD
  • POLYMERASE-CHAIN-REACTION
  • INTERNAL TRANSCRIBED SPACER
  • CYTOCHROME-OXIDASE-I
  • SNAIL HOSTS
  • BIOMPHALARIA-GLABRATA
  • GENETIC-VARIATION
  • ARBITRARY PRIMERS
  • RIBOSOMAL DNA
  • GENUS BULINUS

Cite this

Molecular identification of schistosome intermediate hosts: case studies of Bulinus forskalii group species (Gastropoda : Planorbidae) from Central and East Africa. / Jones, C S ; Noble, L R ; Ouma, J ; Kariuki, H C ; Mimpfoundi, R ; Brown, D S ; Rollinson, D .

In: Biological Journal of the Linnean Society, Vol. 68, 1999, p. 215-240.

Research output: Contribution to journalArticle

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abstract = "African freshwater snails of the genus Bullinus acr as intermediate hosts for schistosomes, trematode parasites responsible for medical and veterinary forms of schitosomiasis. The relationship between these snails and their parasites is an intricate one, with particular species of snail susceptible to infection only by certain species of schistosome. In common with other self-fertile hermaphrodite gastropods, Bulinus consists of a number of closely related species complexes with restricted gene flow between populations of each taxon. Consequently, despite their medical and veterinary importance as intermediate hosts, unambiguous identification and differentiation of planorbid snails such as these remains problematic, often confounding attempts to define the distribution and evolutionary relationships of conchologically similar taxa. Here we consider how morphological methods of discrimination can be used in conjunction with molecular based approaches to improve snail identification. thereby achieving a better understanding of the epidemiology of schistosomiasis. Data are presented from Central and East. African taxa which illustrate how PCR-bared methods have begun to be used in combination with traditional analyses in an integrated approach to characterize the genus Bulinus, specifically the B. forskalii species group. particular emphasis is given to the analyses of Random Amplified Polymorphic DNA (RAPDs) and die mitochondrial gene cytochrome oxidase I (COI). (C) 1999 The Linnean Society of London.",
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T1 - Molecular identification of schistosome intermediate hosts: case studies of Bulinus forskalii group species (Gastropoda : Planorbidae) from Central and East Africa

AU - Jones, C S

AU - Noble, L R

AU - Ouma, J

AU - Kariuki, H C

AU - Mimpfoundi, R

AU - Brown, D S

AU - Rollinson, D

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N2 - African freshwater snails of the genus Bullinus acr as intermediate hosts for schistosomes, trematode parasites responsible for medical and veterinary forms of schitosomiasis. The relationship between these snails and their parasites is an intricate one, with particular species of snail susceptible to infection only by certain species of schistosome. In common with other self-fertile hermaphrodite gastropods, Bulinus consists of a number of closely related species complexes with restricted gene flow between populations of each taxon. Consequently, despite their medical and veterinary importance as intermediate hosts, unambiguous identification and differentiation of planorbid snails such as these remains problematic, often confounding attempts to define the distribution and evolutionary relationships of conchologically similar taxa. Here we consider how morphological methods of discrimination can be used in conjunction with molecular based approaches to improve snail identification. thereby achieving a better understanding of the epidemiology of schistosomiasis. Data are presented from Central and East. African taxa which illustrate how PCR-bared methods have begun to be used in combination with traditional analyses in an integrated approach to characterize the genus Bulinus, specifically the B. forskalii species group. particular emphasis is given to the analyses of Random Amplified Polymorphic DNA (RAPDs) and die mitochondrial gene cytochrome oxidase I (COI). (C) 1999 The Linnean Society of London.

AB - African freshwater snails of the genus Bullinus acr as intermediate hosts for schistosomes, trematode parasites responsible for medical and veterinary forms of schitosomiasis. The relationship between these snails and their parasites is an intricate one, with particular species of snail susceptible to infection only by certain species of schistosome. In common with other self-fertile hermaphrodite gastropods, Bulinus consists of a number of closely related species complexes with restricted gene flow between populations of each taxon. Consequently, despite their medical and veterinary importance as intermediate hosts, unambiguous identification and differentiation of planorbid snails such as these remains problematic, often confounding attempts to define the distribution and evolutionary relationships of conchologically similar taxa. Here we consider how morphological methods of discrimination can be used in conjunction with molecular based approaches to improve snail identification. thereby achieving a better understanding of the epidemiology of schistosomiasis. Data are presented from Central and East. African taxa which illustrate how PCR-bared methods have begun to be used in combination with traditional analyses in an integrated approach to characterize the genus Bulinus, specifically the B. forskalii species group. particular emphasis is given to the analyses of Random Amplified Polymorphic DNA (RAPDs) and die mitochondrial gene cytochrome oxidase I (COI). (C) 1999 The Linnean Society of London.

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KW - cytochrome oxidase subunit I

KW - COI

KW - Randomly Amplified Polymorphic DNA

KW - RAPDs

KW - arbitrary primers

KW - phylogenetics

KW - morphology

KW - morphometrics

KW - POLYMORPHIC DNA RAPD

KW - POLYMERASE-CHAIN-REACTION

KW - INTERNAL TRANSCRIBED SPACER

KW - CYTOCHROME-OXIDASE-I

KW - SNAIL HOSTS

KW - BIOMPHALARIA-GLABRATA

KW - GENETIC-VARIATION

KW - ARBITRARY PRIMERS

KW - RIBOSOMAL DNA

KW - GENUS BULINUS

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VL - 68

SP - 215

EP - 240

JO - Biological Journal of the Linnean Society

JF - Biological Journal of the Linnean Society

SN - 0024-4066

ER -