Molecular phylogenetics of porcini mushrooms (Boletus section Boletus)

Bryn T. M. Dentinger*, Joseph F. Ammirati, Ernst E. Both, Dennis E. Desjardin, Roy E. Halling, Terry W. Henkel, Pierre-Arthur Moreau, Eiji Nagasawa, Kasem Soytong, Andy F. Taylor, Roy Watling, Jean-Marc Moncalvo, David J. McLaughlin

*Corresponding author for this work

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Porcini (Boletus section Boletus: Boletaceae: Boletineae: Boletales) are a conspicuous group of wild, edible mushrooms characterized by fleshy fruiting bodies with a poroid hymenophore that is "stuffed" with white hyphae when young. Their reported distribution is with ectomycorrhizal plants throughout the Northern Hemisphere. Little progress has been made on the systematics of this group using modern molecular phylogenetic tools because sampling has been limited primarily to European species and the genes employed were insufficient to resolve the phylogeny. We examined the evolutionary history of porcini by using a global geographic sampling of most known species, new discoveries from little explored areas, and multiple genes. We used 78 sequences from the fast-evolving nuclear internal transcribed spacers and are able to recognize 18 reciprocally monophyletic species. To address whether or not porcini form a monophyletic group, we compiled a broadly sampled dataset of 41 taxa, including other members of the Boletineae, and used separate and combined phylogenetic analysis of sequences from the nuclear large subunit ribosomal DNA, the largest subunit of RNA polymerase II, and the mitochondrial ATPase subunit six gene. Contrary to previous studies, our separate and combined phylogenetic analyses support the monophyly of porcini. We also report the discovery of two taxa that expand the known distribution of porcini to Australia and Thailand and have ancient phylogenetic connections to the rest of the group. A relaxed molecular clock analysis with these new taxa dates the origin of porcini to between 42 and 54 million years ago, coinciding with the initial diversification of angiosperms, during the Eocene epoch when the climate was warm and humid. These results reveal an unexpected diversity, distribution, and ancient origin of a group of commercially valuable mushrooms that may provide an economic incentive for conservation and support the hypothesis of a tropical origin of the ectomycorrhizal symbiosis. (C) 2010 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1276-1292
Number of pages17
JournalMolecular Phylogenetics and Evolution
Volume57
Issue number3
Early online date21 Oct 2010
DOIs
Publication statusPublished - Dec 2010

Keywords

  • ectomycorrhizal fungi
  • partial veil
  • stuffed pores
  • synapomorphy
  • biogeography
  • inference
  • molecular systematics
  • ITS
  • species complex
  • boletales
  • RPB1
  • mixed models
  • molecular clock
  • long-distance dispersal
  • sustainable non-timber forest product
  • systematics
  • identification
  • conservation
  • basidiomycota
  • edulis
  • high-throughput
  • evolution
  • ATP6

Cite this

Dentinger, B. T. M., Ammirati, J. F., Both, E. E., Desjardin, D. E., Halling, R. E., Henkel, T. W., ... McLaughlin, D. J. (2010). Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Molecular Phylogenetics and Evolution, 57(3), 1276-1292. https://doi.org/10.1016/j.ympev.2010.10.004

Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). / Dentinger, Bryn T. M.; Ammirati, Joseph F.; Both, Ernst E.; Desjardin, Dennis E.; Halling, Roy E.; Henkel, Terry W.; Moreau, Pierre-Arthur; Nagasawa, Eiji; Soytong, Kasem; Taylor, Andy F.; Watling, Roy; Moncalvo, Jean-Marc; McLaughlin, David J.

In: Molecular Phylogenetics and Evolution, Vol. 57, No. 3, 12.2010, p. 1276-1292.

Research output: Contribution to journalArticle

Dentinger, BTM, Ammirati, JF, Both, EE, Desjardin, DE, Halling, RE, Henkel, TW, Moreau, P-A, Nagasawa, E, Soytong, K, Taylor, AF, Watling, R, Moncalvo, J-M & McLaughlin, DJ 2010, 'Molecular phylogenetics of porcini mushrooms (Boletus section Boletus)', Molecular Phylogenetics and Evolution, vol. 57, no. 3, pp. 1276-1292. https://doi.org/10.1016/j.ympev.2010.10.004
Dentinger BTM, Ammirati JF, Both EE, Desjardin DE, Halling RE, Henkel TW et al. Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Molecular Phylogenetics and Evolution. 2010 Dec;57(3):1276-1292. https://doi.org/10.1016/j.ympev.2010.10.004
Dentinger, Bryn T. M. ; Ammirati, Joseph F. ; Both, Ernst E. ; Desjardin, Dennis E. ; Halling, Roy E. ; Henkel, Terry W. ; Moreau, Pierre-Arthur ; Nagasawa, Eiji ; Soytong, Kasem ; Taylor, Andy F. ; Watling, Roy ; Moncalvo, Jean-Marc ; McLaughlin, David J. / Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). In: Molecular Phylogenetics and Evolution. 2010 ; Vol. 57, No. 3. pp. 1276-1292.
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AU - Halling, Roy E.

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KW - partial veil

KW - stuffed pores

KW - synapomorphy

KW - biogeography

KW - inference

KW - molecular systematics

KW - ITS

KW - species complex

KW - boletales

KW - RPB1

KW - mixed models

KW - molecular clock

KW - long-distance dispersal

KW - sustainable non-timber forest product

KW - systematics

KW - identification

KW - conservation

KW - basidiomycota

KW - edulis

KW - high-throughput

KW - evolution

KW - ATP6

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DO - 10.1016/j.ympev.2010.10.004

M3 - Article

VL - 57

SP - 1276

EP - 1292

JO - Molecular Phylogenetics and Evolution

JF - Molecular Phylogenetics and Evolution

SN - 1055-7903

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