Molecular profiling of signet ring cell colorectal cancer provides a strong rationale for genomic targeted and immune checkpoint inhibitor therapies

Muhammad A. Alvi, Maurice B. Loughrey, Philip Dunne, Stephen McQuaid, Richard Turkington, Marc-Aurel Fuchs, Claire McGready, Victoria Bingham, Brendan Pang, Wendy Moore, Perry Maxwell, Mark Lawler, Jacqueline A. James, Graeme I. Murray, Richard H. Wilson, Manuel Salto-Tellez

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Abstract

BACKGROUND: Signet ring cell colorectal cancer (SRCCa) has a bleak prognosis. Employing molecular pathology techniques we investigated the potential of precision medicine in this disease.

METHODS: Using test (n=26) and validation (n=18) cohorts, analysis of mutations, DNA methylation and transcriptome was carried out. Microsatellite instability (MSI) status was established and immunohistochemistry (IHC) was used to test for adaptive immunity (CD3) and the immune checkpoint PDL1.

RESULTS: DNA methylation data split the cohorts into hypermethylated (n=18, 41%) and hypomethylated groups (n=26, 59%). The hypermethylated group predominant in the proximal colon was enriched for CpG island methylator phenotype (CIMP), BRAF V600E mutation and MSI (P<0.001). These cases also had a high CD3(+) immune infiltrate (P<0.001) and expressed PDL1 (P=0.03 in intra-tumoural lymphoid cells). The hypomethylated group predominant in the distal colon did not show any characteristic molecular features. We also detected a common targetable KIT mutation (c.1621A>C) across both groups. No statistically significant difference in outcome was observed between the two groups.

CONCLUSIONS: Our data show that SRCCa phenotype comprises two distinct genotypes. The MSI(+)/CIMP(+)/BRAF V600E(+)/CD3(+)/PDL1(+) hypermethylated genotype is an ideal candidate for immune checkpoint inhibitor therapy. In addition, one fourth of SRCCa cases can potentially be targeted by KIT inhibitors.British Journal of Cancer advance online publication, 8 June 2017; doi:10.1038/bjc.2017.168 www.bjcancer.com.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalBritish Journal of Cancer
Volume117
Issue number2
Early online date8 Jun 2017
DOIs
Publication statusPublished - 11 Jul 2017

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Microsatellite Instability
Colorectal Neoplasms
CpG Islands
DNA Methylation
Phenotype
Genotype
Precision Medicine
Mutation
Molecular Pathology
Adaptive Immunity
Transcriptome
Publications
Colon
Cohort Studies
Therapeutics
Immunohistochemistry
Neoplasms

Keywords

  • KIT
  • genotype
  • PDL1

Cite this

Molecular profiling of signet ring cell colorectal cancer provides a strong rationale for genomic targeted and immune checkpoint inhibitor therapies. / Alvi, Muhammad A.; Loughrey, Maurice B.; Dunne, Philip; McQuaid, Stephen; Turkington, Richard; Fuchs, Marc-Aurel; McGready, Claire; Bingham, Victoria; Pang, Brendan; Moore, Wendy; Maxwell, Perry; Lawler, Mark; James, Jacqueline A.; Murray, Graeme I.; Wilson, Richard H.; Salto-Tellez, Manuel.

In: British Journal of Cancer, Vol. 117, No. 2, 11.07.2017, p. 203-209.

Research output: Contribution to journalArticle

Alvi, MA, Loughrey, MB, Dunne, P, McQuaid, S, Turkington, R, Fuchs, M-A, McGready, C, Bingham, V, Pang, B, Moore, W, Maxwell, P, Lawler, M, James, JA, Murray, GI, Wilson, RH & Salto-Tellez, M 2017, 'Molecular profiling of signet ring cell colorectal cancer provides a strong rationale for genomic targeted and immune checkpoint inhibitor therapies', British Journal of Cancer, vol. 117, no. 2, pp. 203-209. https://doi.org/10.1038/bjc.2017.168
Alvi, Muhammad A. ; Loughrey, Maurice B. ; Dunne, Philip ; McQuaid, Stephen ; Turkington, Richard ; Fuchs, Marc-Aurel ; McGready, Claire ; Bingham, Victoria ; Pang, Brendan ; Moore, Wendy ; Maxwell, Perry ; Lawler, Mark ; James, Jacqueline A. ; Murray, Graeme I. ; Wilson, Richard H. ; Salto-Tellez, Manuel. / Molecular profiling of signet ring cell colorectal cancer provides a strong rationale for genomic targeted and immune checkpoint inhibitor therapies. In: British Journal of Cancer. 2017 ; Vol. 117, No. 2. pp. 203-209.
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abstract = "BACKGROUND: Signet ring cell colorectal cancer (SRCCa) has a bleak prognosis. Employing molecular pathology techniques we investigated the potential of precision medicine in this disease.METHODS: Using test (n=26) and validation (n=18) cohorts, analysis of mutations, DNA methylation and transcriptome was carried out. Microsatellite instability (MSI) status was established and immunohistochemistry (IHC) was used to test for adaptive immunity (CD3) and the immune checkpoint PDL1.RESULTS: DNA methylation data split the cohorts into hypermethylated (n=18, 41{\%}) and hypomethylated groups (n=26, 59{\%}). The hypermethylated group predominant in the proximal colon was enriched for CpG island methylator phenotype (CIMP), BRAF V600E mutation and MSI (P<0.001). These cases also had a high CD3(+) immune infiltrate (P<0.001) and expressed PDL1 (P=0.03 in intra-tumoural lymphoid cells). The hypomethylated group predominant in the distal colon did not show any characteristic molecular features. We also detected a common targetable KIT mutation (c.1621A>C) across both groups. No statistically significant difference in outcome was observed between the two groups.CONCLUSIONS: Our data show that SRCCa phenotype comprises two distinct genotypes. The MSI(+)/CIMP(+)/BRAF V600E(+)/CD3(+)/PDL1(+) hypermethylated genotype is an ideal candidate for immune checkpoint inhibitor therapy. In addition, one fourth of SRCCa cases can potentially be targeted by KIT inhibitors.British Journal of Cancer advance online publication, 8 June 2017; doi:10.1038/bjc.2017.168 www.bjcancer.com.",
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author = "Alvi, {Muhammad A.} and Loughrey, {Maurice B.} and Philip Dunne and Stephen McQuaid and Richard Turkington and Marc-Aurel Fuchs and Claire McGready and Victoria Bingham and Brendan Pang and Wendy Moore and Perry Maxwell and Mark Lawler and James, {Jacqueline A.} and Murray, {Graeme I.} and Wilson, {Richard H.} and Manuel Salto-Tellez",
note = "We would like to thank all patients whose samples were used in this study. We are also thankful to the Northern Ireland Biobank and Grampian Biorepository for providing us with tissue blocks and patient data; and Dr HG Coleman (Queen’s University Belfast) for her advice on statistical analyses. This work has been carried out with financial support from Cancer Research UK (grant: C11512/A18067), Experimental Cancer Medicine Centre Network (grant: C36697/A15590 from Cancer Research UK and the NI Health and Social Care Research and Development Division), the Sean Crummey Memorial Fund and the Tom Simms Memorial Fund. The Northern Ireland Biobank is funded by HSC Research and Development Division of the Public Health Agency in Northern Ireland and Cancer Research UK through the Belfast CRUK Centre and the Northern Ireland Experimental Cancer Medicine Centre; additional support was received from Friends of the Cancer Centre. The Northern Ireland Molecular Pathology Laboratory which is responsible for creating resources for the Northern Ireland Biobank has received funding from Cancer Research UK, Friends of the Cancer Centre and Sean Crummey Foundation.",
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T1 - Molecular profiling of signet ring cell colorectal cancer provides a strong rationale for genomic targeted and immune checkpoint inhibitor therapies

AU - Alvi, Muhammad A.

AU - Loughrey, Maurice B.

AU - Dunne, Philip

AU - McQuaid, Stephen

AU - Turkington, Richard

AU - Fuchs, Marc-Aurel

AU - McGready, Claire

AU - Bingham, Victoria

AU - Pang, Brendan

AU - Moore, Wendy

AU - Maxwell, Perry

AU - Lawler, Mark

AU - James, Jacqueline A.

AU - Murray, Graeme I.

AU - Wilson, Richard H.

AU - Salto-Tellez, Manuel

N1 - We would like to thank all patients whose samples were used in this study. We are also thankful to the Northern Ireland Biobank and Grampian Biorepository for providing us with tissue blocks and patient data; and Dr HG Coleman (Queen’s University Belfast) for her advice on statistical analyses. This work has been carried out with financial support from Cancer Research UK (grant: C11512/A18067), Experimental Cancer Medicine Centre Network (grant: C36697/A15590 from Cancer Research UK and the NI Health and Social Care Research and Development Division), the Sean Crummey Memorial Fund and the Tom Simms Memorial Fund. The Northern Ireland Biobank is funded by HSC Research and Development Division of the Public Health Agency in Northern Ireland and Cancer Research UK through the Belfast CRUK Centre and the Northern Ireland Experimental Cancer Medicine Centre; additional support was received from Friends of the Cancer Centre. The Northern Ireland Molecular Pathology Laboratory which is responsible for creating resources for the Northern Ireland Biobank has received funding from Cancer Research UK, Friends of the Cancer Centre and Sean Crummey Foundation.

PY - 2017/7/11

Y1 - 2017/7/11

N2 - BACKGROUND: Signet ring cell colorectal cancer (SRCCa) has a bleak prognosis. Employing molecular pathology techniques we investigated the potential of precision medicine in this disease.METHODS: Using test (n=26) and validation (n=18) cohorts, analysis of mutations, DNA methylation and transcriptome was carried out. Microsatellite instability (MSI) status was established and immunohistochemistry (IHC) was used to test for adaptive immunity (CD3) and the immune checkpoint PDL1.RESULTS: DNA methylation data split the cohorts into hypermethylated (n=18, 41%) and hypomethylated groups (n=26, 59%). The hypermethylated group predominant in the proximal colon was enriched for CpG island methylator phenotype (CIMP), BRAF V600E mutation and MSI (P<0.001). These cases also had a high CD3(+) immune infiltrate (P<0.001) and expressed PDL1 (P=0.03 in intra-tumoural lymphoid cells). The hypomethylated group predominant in the distal colon did not show any characteristic molecular features. We also detected a common targetable KIT mutation (c.1621A>C) across both groups. No statistically significant difference in outcome was observed between the two groups.CONCLUSIONS: Our data show that SRCCa phenotype comprises two distinct genotypes. The MSI(+)/CIMP(+)/BRAF V600E(+)/CD3(+)/PDL1(+) hypermethylated genotype is an ideal candidate for immune checkpoint inhibitor therapy. In addition, one fourth of SRCCa cases can potentially be targeted by KIT inhibitors.British Journal of Cancer advance online publication, 8 June 2017; doi:10.1038/bjc.2017.168 www.bjcancer.com.

AB - BACKGROUND: Signet ring cell colorectal cancer (SRCCa) has a bleak prognosis. Employing molecular pathology techniques we investigated the potential of precision medicine in this disease.METHODS: Using test (n=26) and validation (n=18) cohorts, analysis of mutations, DNA methylation and transcriptome was carried out. Microsatellite instability (MSI) status was established and immunohistochemistry (IHC) was used to test for adaptive immunity (CD3) and the immune checkpoint PDL1.RESULTS: DNA methylation data split the cohorts into hypermethylated (n=18, 41%) and hypomethylated groups (n=26, 59%). The hypermethylated group predominant in the proximal colon was enriched for CpG island methylator phenotype (CIMP), BRAF V600E mutation and MSI (P<0.001). These cases also had a high CD3(+) immune infiltrate (P<0.001) and expressed PDL1 (P=0.03 in intra-tumoural lymphoid cells). The hypomethylated group predominant in the distal colon did not show any characteristic molecular features. We also detected a common targetable KIT mutation (c.1621A>C) across both groups. No statistically significant difference in outcome was observed between the two groups.CONCLUSIONS: Our data show that SRCCa phenotype comprises two distinct genotypes. The MSI(+)/CIMP(+)/BRAF V600E(+)/CD3(+)/PDL1(+) hypermethylated genotype is an ideal candidate for immune checkpoint inhibitor therapy. In addition, one fourth of SRCCa cases can potentially be targeted by KIT inhibitors.British Journal of Cancer advance online publication, 8 June 2017; doi:10.1038/bjc.2017.168 www.bjcancer.com.

KW - KIT

KW - genotype

KW - PDL1

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DO - 10.1038/bjc.2017.168

M3 - Article

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EP - 209

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

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