morganmehargImmunoperocidase detection of metallothioneins and ligand arsenic complexation in the earthworm Lumbricus rubellus inhabiting arsenic-rich soil.

C. Langdon, C. winters, J. M. charnock, T. G. piearce, P. R. Lee, K. T. semple, Andrew Alexander Meharg, S. R. sturzenbaum, A. J. morgan

Research output: Contribution to journalArticle

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Abstract

Although earthworms have been found to inhabit arsenic-rich soils in the U.K., the mode of arsenic detoxification is currently unknown. Biochemical analyses and subcellular localization studies have indicated that As3+-thiol complexes may be involved; however, it is not known whether arsenic is capable of inducing the expression of metallothionein (MT) in earthworms. The specific aims of this paper were (a) to detect and gain an atomic characterization of ligand complexing by X-ray absorption spectrometry (XAS), and (b) to employ a polyclonal antibody raised against an earthworm MT isoform (w-MT2) to detect and localize the metalloprotein by immunoperoxidase histochemistry in the tissues of earthworms sampled from arsenic-rich soil. Data suggested that the proportion of arsenate to sulfur-bound species varies within specific earthworm tissues. Although some arsenic appeared to be in the form of arsenobetaine, the arsenic within the chlorogogenous tissue was predominantly coordinated with S in the form of -SH groups. This suggests the presence of an As::MT complex. Indeed, MT was detectable with a distinctly localized tissue and cellular distribution. While MT was not detectable in the surface epithelium or in the body wall musculature, immunoperoxidase histochemistry identified the presence of MT in chloragocytes around blood vessels, within the typhlosolar fold, and in the peri-intestinal region. Focal immunostaining was also detectable in a cohort of cells in the intestinal wall. The results of this study support the hypothesis that arsenic induces MT expression and is sequestered by the metalloprotein in certain target cells and tissues.

Original languageEnglish
Pages (from-to)2042-2048
Number of pages6
JournalEnvironmental Science & Technology
Volume39
DOIs
Publication statusPublished - 2005

Keywords

  • CURVED-WAVE THEORY
  • POULTRY LITTER
  • CADMIUM
  • RESISTANCE
  • TOXICITY
  • COPPER
  • IDENTIFICATION
  • DETOXIFICATION
  • TISSUES
  • MICE

Cite this

morganmehargImmunoperocidase detection of metallothioneins and ligand arsenic complexation in the earthworm Lumbricus rubellus inhabiting arsenic-rich soil. / Langdon, C.; winters, C.; charnock, J. M.; piearce, T. G.; Lee, P. R.; semple, K. T.; Meharg, Andrew Alexander; sturzenbaum, S. R.; morgan, A. J.

In: Environmental Science & Technology, Vol. 39, 2005, p. 2042-2048.

Research output: Contribution to journalArticle

Langdon, C, winters, C, charnock, JM, piearce, TG, Lee, PR, semple, KT, Meharg, AA, sturzenbaum, SR & morgan, AJ 2005, 'morganmehargImmunoperocidase detection of metallothioneins and ligand arsenic complexation in the earthworm Lumbricus rubellus inhabiting arsenic-rich soil.', Environmental Science & Technology, vol. 39, pp. 2042-2048. https://doi.org/10.1021/es0490471
Langdon, C. ; winters, C. ; charnock, J. M. ; piearce, T. G. ; Lee, P. R. ; semple, K. T. ; Meharg, Andrew Alexander ; sturzenbaum, S. R. ; morgan, A. J. / morganmehargImmunoperocidase detection of metallothioneins and ligand arsenic complexation in the earthworm Lumbricus rubellus inhabiting arsenic-rich soil. In: Environmental Science & Technology. 2005 ; Vol. 39. pp. 2042-2048.
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abstract = "Although earthworms have been found to inhabit arsenic-rich soils in the U.K., the mode of arsenic detoxification is currently unknown. Biochemical analyses and subcellular localization studies have indicated that As3+-thiol complexes may be involved; however, it is not known whether arsenic is capable of inducing the expression of metallothionein (MT) in earthworms. The specific aims of this paper were (a) to detect and gain an atomic characterization of ligand complexing by X-ray absorption spectrometry (XAS), and (b) to employ a polyclonal antibody raised against an earthworm MT isoform (w-MT2) to detect and localize the metalloprotein by immunoperoxidase histochemistry in the tissues of earthworms sampled from arsenic-rich soil. Data suggested that the proportion of arsenate to sulfur-bound species varies within specific earthworm tissues. Although some arsenic appeared to be in the form of arsenobetaine, the arsenic within the chlorogogenous tissue was predominantly coordinated with S in the form of -SH groups. This suggests the presence of an As::MT complex. Indeed, MT was detectable with a distinctly localized tissue and cellular distribution. While MT was not detectable in the surface epithelium or in the body wall musculature, immunoperoxidase histochemistry identified the presence of MT in chloragocytes around blood vessels, within the typhlosolar fold, and in the peri-intestinal region. Focal immunostaining was also detectable in a cohort of cells in the intestinal wall. The results of this study support the hypothesis that arsenic induces MT expression and is sequestered by the metalloprotein in certain target cells and tissues.",
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AU - Langdon, C.

AU - winters, C.

AU - charnock, J. M.

AU - piearce, T. G.

AU - Lee, P. R.

AU - semple, K. T.

AU - Meharg, Andrew Alexander

AU - sturzenbaum, S. R.

AU - morgan, A. J.

PY - 2005

Y1 - 2005

N2 - Although earthworms have been found to inhabit arsenic-rich soils in the U.K., the mode of arsenic detoxification is currently unknown. Biochemical analyses and subcellular localization studies have indicated that As3+-thiol complexes may be involved; however, it is not known whether arsenic is capable of inducing the expression of metallothionein (MT) in earthworms. The specific aims of this paper were (a) to detect and gain an atomic characterization of ligand complexing by X-ray absorption spectrometry (XAS), and (b) to employ a polyclonal antibody raised against an earthworm MT isoform (w-MT2) to detect and localize the metalloprotein by immunoperoxidase histochemistry in the tissues of earthworms sampled from arsenic-rich soil. Data suggested that the proportion of arsenate to sulfur-bound species varies within specific earthworm tissues. Although some arsenic appeared to be in the form of arsenobetaine, the arsenic within the chlorogogenous tissue was predominantly coordinated with S in the form of -SH groups. This suggests the presence of an As::MT complex. Indeed, MT was detectable with a distinctly localized tissue and cellular distribution. While MT was not detectable in the surface epithelium or in the body wall musculature, immunoperoxidase histochemistry identified the presence of MT in chloragocytes around blood vessels, within the typhlosolar fold, and in the peri-intestinal region. Focal immunostaining was also detectable in a cohort of cells in the intestinal wall. The results of this study support the hypothesis that arsenic induces MT expression and is sequestered by the metalloprotein in certain target cells and tissues.

AB - Although earthworms have been found to inhabit arsenic-rich soils in the U.K., the mode of arsenic detoxification is currently unknown. Biochemical analyses and subcellular localization studies have indicated that As3+-thiol complexes may be involved; however, it is not known whether arsenic is capable of inducing the expression of metallothionein (MT) in earthworms. The specific aims of this paper were (a) to detect and gain an atomic characterization of ligand complexing by X-ray absorption spectrometry (XAS), and (b) to employ a polyclonal antibody raised against an earthworm MT isoform (w-MT2) to detect and localize the metalloprotein by immunoperoxidase histochemistry in the tissues of earthworms sampled from arsenic-rich soil. Data suggested that the proportion of arsenate to sulfur-bound species varies within specific earthworm tissues. Although some arsenic appeared to be in the form of arsenobetaine, the arsenic within the chlorogogenous tissue was predominantly coordinated with S in the form of -SH groups. This suggests the presence of an As::MT complex. Indeed, MT was detectable with a distinctly localized tissue and cellular distribution. While MT was not detectable in the surface epithelium or in the body wall musculature, immunoperoxidase histochemistry identified the presence of MT in chloragocytes around blood vessels, within the typhlosolar fold, and in the peri-intestinal region. Focal immunostaining was also detectable in a cohort of cells in the intestinal wall. The results of this study support the hypothesis that arsenic induces MT expression and is sequestered by the metalloprotein in certain target cells and tissues.

KW - CURVED-WAVE THEORY

KW - POULTRY LITTER

KW - CADMIUM

KW - RESISTANCE

KW - TOXICITY

KW - COPPER

KW - IDENTIFICATION

KW - DETOXIFICATION

KW - TISSUES

KW - MICE

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DO - 10.1021/es0490471

M3 - Article

VL - 39

SP - 2042

EP - 2048

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

ER -