Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane

F Vandenabeele, Cosimo De Bari, M Moreels, I Lambrichts, F Dell'Accio, P L Lippens, F P Luyten

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.
Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalArchives of Histology and Cytology
Volume66
Issue number2
DOIs
Publication statusPublished - 1 May 2003

Keywords

  • Adipocytes
  • Cell Differentiation
  • Cell Lineage
  • Cells, Cultured
  • Chondrocytes
  • Exocytosis
  • Fibroblasts
  • Humans
  • Immunohistochemistry
  • Mesoderm
  • Oligodendroglia
  • Pseudopodia
  • Pulmonary Surfactant-Associated Protein A
  • Stem Cells
  • Synovial Membrane

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