Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane

F Vandenabeele, Cosimo De Bari, M Moreels, I Lambrichts, F Dell'Accio, P L Lippens, F P Luyten

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.
Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalArchives of Histology and Cytology
Volume66
Issue number2
DOIs
Publication statusPublished - 1 May 2003

Fingerprint

Synovial Membrane
Pulmonary Surfactant-Associated Protein A
Fibroblasts
Mesenchymal Stromal Cells
Multipotent Stem Cells
Exocytosis
Electron Microscopy
Cell Culture Techniques
Immunohistochemistry
Phenotype
Population
Synoviocytes

Keywords

  • Adipocytes
  • Cell Differentiation
  • Cell Lineage
  • Cells, Cultured
  • Chondrocytes
  • Exocytosis
  • Fibroblasts
  • Humans
  • Immunohistochemistry
  • Mesoderm
  • Oligodendroglia
  • Pseudopodia
  • Pulmonary Surfactant-Associated Protein A
  • Stem Cells
  • Synovial Membrane

Cite this

Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane. / Vandenabeele, F; De Bari, Cosimo; Moreels, M; Lambrichts, I; Dell'Accio, F; Lippens, P L; Luyten, F P.

In: Archives of Histology and Cytology, Vol. 66, No. 2, 01.05.2003, p. 145-153.

Research output: Contribution to journalArticle

Vandenabeele, F ; De Bari, Cosimo ; Moreels, M ; Lambrichts, I ; Dell'Accio, F ; Lippens, P L ; Luyten, F P. / Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane. In: Archives of Histology and Cytology. 2003 ; Vol. 66, No. 2. pp. 145-153.
@article{f8a02d2be73848f6a6546ff3a7f17466,
title = "Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane",
abstract = "The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.",
keywords = "Adipocytes, Cell Differentiation, Cell Lineage, Cells, Cultured, Chondrocytes, Exocytosis, Fibroblasts, Humans, Immunohistochemistry, Mesoderm, Oligodendroglia, Pseudopodia, Pulmonary Surfactant-Associated Protein A, Stem Cells, Synovial Membrane",
author = "F Vandenabeele and {De Bari}, Cosimo and M Moreels and I Lambrichts and F Dell'Accio and Lippens, {P L} and Luyten, {F P}",
year = "2003",
month = "5",
day = "1",
doi = "10.1679/aohc.66.145",
language = "English",
volume = "66",
pages = "145--153",
journal = "Archives of Histology and Cytology",
issn = "0914-9465",
publisher = "Japan Society of Histological Documentation",
number = "2",

}

TY - JOUR

T1 - Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane

AU - Vandenabeele, F

AU - De Bari, Cosimo

AU - Moreels, M

AU - Lambrichts, I

AU - Dell'Accio, F

AU - Lippens, P L

AU - Luyten, F P

PY - 2003/5/1

Y1 - 2003/5/1

N2 - The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.

AB - The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.

KW - Adipocytes

KW - Cell Differentiation

KW - Cell Lineage

KW - Cells, Cultured

KW - Chondrocytes

KW - Exocytosis

KW - Fibroblasts

KW - Humans

KW - Immunohistochemistry

KW - Mesoderm

KW - Oligodendroglia

KW - Pseudopodia

KW - Pulmonary Surfactant-Associated Protein A

KW - Stem Cells

KW - Synovial Membrane

U2 - 10.1679/aohc.66.145

DO - 10.1679/aohc.66.145

M3 - Article

VL - 66

SP - 145

EP - 153

JO - Archives of Histology and Cytology

JF - Archives of Histology and Cytology

SN - 0914-9465

IS - 2

ER -