Mouse granulated metrial gland cells require contact with stromal cells to maintain viability

I J Stewart

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Granulated metrial gland (GMG) cells differentiate in the uterine wall in pregnancy in mice but the mechanisms which control their differentiation and maintenance are unknown. In vivo, GMG cells share an intimate association with fibroblast-like stromal cells. The importance of this association has been assessed by examining the effects of withdrawal of stromal cell contact on GMG cell maintenance in vitro. When single cell suspensions of cells were prepared from mouse metrial glands there was a steady decline in numbers with days of culture but usually some remained at 7 d of culture. The ability of metrial gland cells to kill Wehi 164 target cells in Cr-51-release cytotoxicity assays was retained by cells cultured for at least 3 d. When explants of metrial gland were maintained in culture to allow GMG cell migration onto the culture flask, the attached GMG cells were lost by 1 d later. Overall, these results suggest that a juxtacrine regulatory mechanism maintains GMG cells. The rapid loss of unsupported GMG cells in culture has major implications in the design of assays to examine GMG cell function.

    Original languageEnglish
    Pages (from-to)495-502
    Number of pages8
    JournalJournal of Anatomy
    Volume197
    Publication statusPublished - 2000

    Keywords

    • granulated metrial gland cells
    • uterus
    • cell viability
    • NATURAL-KILLER-CELLS
    • UTERUS
    • PREGNANCY
    • PROGESTERONE
    • DIFFERENTIATION
    • RECEPTORS
    • EXPLANTS
    • ESTROGEN
    • MICE
    • RAT

    Cite this

    Mouse granulated metrial gland cells require contact with stromal cells to maintain viability. / Stewart, I J .

    In: Journal of Anatomy, Vol. 197, 2000, p. 495-502.

    Research output: Contribution to journalArticle

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    abstract = "Granulated metrial gland (GMG) cells differentiate in the uterine wall in pregnancy in mice but the mechanisms which control their differentiation and maintenance are unknown. In vivo, GMG cells share an intimate association with fibroblast-like stromal cells. The importance of this association has been assessed by examining the effects of withdrawal of stromal cell contact on GMG cell maintenance in vitro. When single cell suspensions of cells were prepared from mouse metrial glands there was a steady decline in numbers with days of culture but usually some remained at 7 d of culture. The ability of metrial gland cells to kill Wehi 164 target cells in Cr-51-release cytotoxicity assays was retained by cells cultured for at least 3 d. When explants of metrial gland were maintained in culture to allow GMG cell migration onto the culture flask, the attached GMG cells were lost by 1 d later. Overall, these results suggest that a juxtacrine regulatory mechanism maintains GMG cells. The rapid loss of unsupported GMG cells in culture has major implications in the design of assays to examine GMG cell function.",
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    KW - ESTROGEN

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