Movement of the parasitic nematode Phasmarhabditis hermaphrodita in the presence of mucus from the host slug Deroceras reticulatum

Simona Hapca, John Crawford, Robert Rae, Michael John Wilson, Iain Young

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phasmarhabditis hermaphrodita is a parasitic nematode capable of killing several species of slugs including Deroceras reticulatum, the most widespread slug pest in the world. This nematode can control slug infestations in a wide range of crops such as wheat, lettuce and strawberries. Optimization of this biocontrol agent depends on a proper understanding of the interaction between the host and parasite. In this paper, we investigate the response of P. hermaphrodita to the presence of slug mucus on plates of agar. We define an attraction index and find that the nematodes are significantly attracted by filter paper impregnated with slug mucus compared to paper impregnated with water. Second, nematode trails were recorded on a homogeneous layer of technical agar, with or without the presence of the slug mucus. Mucus was applied in two treatments comprising localization on a piece of filter paper and a uniform distribution across the plate. The different mucus treatments induced significantly different effects on the speed of nematode movement and the distribution of the turning angles, as well as the fractal dimension of nematode foraging trail. These results are consistent with the hypothesis that the nematodes exhibit both a chemotactic and chemokinetic response to a signal emanating from slug mucus.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalBiological Control
Volume41
Issue number2
Early online date20 Jan 2007
DOIs
Publication statusPublished - May 2007

Keywords

  • Phasmarhabditis hermaphrodita
  • Deroceras reticulatum
  • chemotaxis
  • chemokinesis
  • attraction index
  • nematode movement
  • turning angle distribution
  • fractal dimension
  • caenorhabditis-elegans
  • behavior
  • cues

Cite this

Movement of the parasitic nematode Phasmarhabditis hermaphrodita in the presence of mucus from the host slug Deroceras reticulatum. / Hapca, Simona; Crawford, John; Rae, Robert; Wilson, Michael John; Young, Iain.

In: Biological Control, Vol. 41, No. 2, 05.2007, p. 223-229.

Research output: Contribution to journalArticle

Hapca, Simona ; Crawford, John ; Rae, Robert ; Wilson, Michael John ; Young, Iain. / Movement of the parasitic nematode Phasmarhabditis hermaphrodita in the presence of mucus from the host slug Deroceras reticulatum. In: Biological Control. 2007 ; Vol. 41, No. 2. pp. 223-229.
@article{ccaddeae4e07423a8691190139f34436,
title = "Movement of the parasitic nematode Phasmarhabditis hermaphrodita in the presence of mucus from the host slug Deroceras reticulatum",
abstract = "Phasmarhabditis hermaphrodita is a parasitic nematode capable of killing several species of slugs including Deroceras reticulatum, the most widespread slug pest in the world. This nematode can control slug infestations in a wide range of crops such as wheat, lettuce and strawberries. Optimization of this biocontrol agent depends on a proper understanding of the interaction between the host and parasite. In this paper, we investigate the response of P. hermaphrodita to the presence of slug mucus on plates of agar. We define an attraction index and find that the nematodes are significantly attracted by filter paper impregnated with slug mucus compared to paper impregnated with water. Second, nematode trails were recorded on a homogeneous layer of technical agar, with or without the presence of the slug mucus. Mucus was applied in two treatments comprising localization on a piece of filter paper and a uniform distribution across the plate. The different mucus treatments induced significantly different effects on the speed of nematode movement and the distribution of the turning angles, as well as the fractal dimension of nematode foraging trail. These results are consistent with the hypothesis that the nematodes exhibit both a chemotactic and chemokinetic response to a signal emanating from slug mucus.",
keywords = "Phasmarhabditis hermaphrodita, Deroceras reticulatum, chemotaxis, chemokinesis, attraction index, nematode movement, turning angle distribution, fractal dimension, caenorhabditis-elegans, behavior, cues",
author = "Simona Hapca and John Crawford and Robert Rae and Wilson, {Michael John} and Iain Young",
year = "2007",
month = "5",
doi = "10.1016/j.biocontrol.2007.01.005",
language = "English",
volume = "41",
pages = "223--229",
journal = "Biological Control",
issn = "1049-9644",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Movement of the parasitic nematode Phasmarhabditis hermaphrodita in the presence of mucus from the host slug Deroceras reticulatum

AU - Hapca, Simona

AU - Crawford, John

AU - Rae, Robert

AU - Wilson, Michael John

AU - Young, Iain

PY - 2007/5

Y1 - 2007/5

N2 - Phasmarhabditis hermaphrodita is a parasitic nematode capable of killing several species of slugs including Deroceras reticulatum, the most widespread slug pest in the world. This nematode can control slug infestations in a wide range of crops such as wheat, lettuce and strawberries. Optimization of this biocontrol agent depends on a proper understanding of the interaction between the host and parasite. In this paper, we investigate the response of P. hermaphrodita to the presence of slug mucus on plates of agar. We define an attraction index and find that the nematodes are significantly attracted by filter paper impregnated with slug mucus compared to paper impregnated with water. Second, nematode trails were recorded on a homogeneous layer of technical agar, with or without the presence of the slug mucus. Mucus was applied in two treatments comprising localization on a piece of filter paper and a uniform distribution across the plate. The different mucus treatments induced significantly different effects on the speed of nematode movement and the distribution of the turning angles, as well as the fractal dimension of nematode foraging trail. These results are consistent with the hypothesis that the nematodes exhibit both a chemotactic and chemokinetic response to a signal emanating from slug mucus.

AB - Phasmarhabditis hermaphrodita is a parasitic nematode capable of killing several species of slugs including Deroceras reticulatum, the most widespread slug pest in the world. This nematode can control slug infestations in a wide range of crops such as wheat, lettuce and strawberries. Optimization of this biocontrol agent depends on a proper understanding of the interaction between the host and parasite. In this paper, we investigate the response of P. hermaphrodita to the presence of slug mucus on plates of agar. We define an attraction index and find that the nematodes are significantly attracted by filter paper impregnated with slug mucus compared to paper impregnated with water. Second, nematode trails were recorded on a homogeneous layer of technical agar, with or without the presence of the slug mucus. Mucus was applied in two treatments comprising localization on a piece of filter paper and a uniform distribution across the plate. The different mucus treatments induced significantly different effects on the speed of nematode movement and the distribution of the turning angles, as well as the fractal dimension of nematode foraging trail. These results are consistent with the hypothesis that the nematodes exhibit both a chemotactic and chemokinetic response to a signal emanating from slug mucus.

KW - Phasmarhabditis hermaphrodita

KW - Deroceras reticulatum

KW - chemotaxis

KW - chemokinesis

KW - attraction index

KW - nematode movement

KW - turning angle distribution

KW - fractal dimension

KW - caenorhabditis-elegans

KW - behavior

KW - cues

U2 - 10.1016/j.biocontrol.2007.01.005

DO - 10.1016/j.biocontrol.2007.01.005

M3 - Article

VL - 41

SP - 223

EP - 229

JO - Biological Control

JF - Biological Control

SN - 1049-9644

IS - 2

ER -