TY - JOUR
T1 - mRNA abundance and half-life measurements.
AU - Brown, A. J.
AU - Sagliocco, F. A.
N1 - The authors are grateful to Andy Bettany and Zaf Zaman for kindly providing data for this chapter, and to Russell Sykes (LabLogic, Sheffield, UK) for providing the photograph for Fig. 3.
PY - 1996/10/25
Y1 - 1996/10/25
N2 - The expression of many genes is regulated at multiple levels. For most genes, the predominant control is at transcription, and this is usually inferred from changes in mRNA levels that respond to specific stimuli. However, changes in mRNA stability may contribute to observed variation m mRNA abundance, and many such changes in mRNA stability probably remain undetected. The relative contributions of post-transcriptional regulatory circuits can only be detected by careful RNA analysis.
AB - The expression of many genes is regulated at multiple levels. For most genes, the predominant control is at transcription, and this is usually inferred from changes in mRNA levels that respond to specific stimuli. However, changes in mRNA stability may contribute to observed variation m mRNA abundance, and many such changes in mRNA stability probably remain undetected. The relative contributions of post-transcriptional regulatory circuits can only be detected by careful RNA analysis.
UR - http://www.scopus.com/inward/record.url?scp=0029690211&partnerID=8YFLogxK
UR - https://link.springer.com/content/pdf/10.1385/0-89603-319-8:277.pdf
M3 - Review article
C2 - 8924989
AN - SCOPUS:0029690211
VL - 53
SP - 277
EP - 295
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -