MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants

Jeffery L. Gustin, Marcello E. Loureiro, Donggiun Kim, Gunnam Na, Marina Tikhonova, David E. Salt

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

The integral membrane protein Thlaspi goesingense metal tolerance protein 1 (TgMTP1) has been suggested to play an important role in Zn hyperaccumulation in T. goesingense. Here, we show that the TgMTP1 protein is accumulated to high levels at the vacuolar membrane in shoot tissue of T. goesingense. TgMTP1 is likely to act in the transport of Zn into the vacuole, enhancing both Zn accumulation and tolerance. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots, we show that TgMTP1, localized at the vacuolar membrane, can drive the enhanced shoot accumulation of Zn by initiating a systemic Zn deficiency response. The systematic response includes increased expression of Zn transporters (ZIP3, ZIP4, ZIP5 and ZIP9) in both shoot and root tissue. Furthermore, shoot-specific accumulation of TgMTP1 at the vacuolar membrane also leads to increased resistance to Zn in A. thaliana, probably through enhanced Zn compartmentalization in the vacuole. Such evidence leads to the conclusion that the high levels of TgMTP1 at the vacuolar membrane in shoot tissue of the Zn hyperaccumulator T. goesingense play a role in both Zn tolerance and enhanced Zn uptake and accumulation, via the activation of a systemic Zn deficiency response.

Original languageEnglish
Pages (from-to)1116-1127
Number of pages12
JournalThe Plant Journal
Volume57
Issue number6
DOIs
Publication statusPublished - Mar 2009

Keywords

  • MTP
  • Thlaspi
  • hyperaccumulation
  • Zn deficiency
  • grafting
  • micronutrient
  • quantitative trait loci
  • zinc-transporter genes
  • thlaspi-caerulescens
  • arabidopsis-thaliana
  • metal homeostasis
  • plasma-membrane
  • cellular compartmentation
  • Halleri
  • cadmium
  • expression

Cite this

MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants. / Gustin, Jeffery L.; Loureiro, Marcello E.; Kim, Donggiun; Na, Gunnam; Tikhonova, Marina; Salt, David E.

In: The Plant Journal, Vol. 57, No. 6, 03.2009, p. 1116-1127.

Research output: Contribution to journalArticle

Gustin, Jeffery L. ; Loureiro, Marcello E. ; Kim, Donggiun ; Na, Gunnam ; Tikhonova, Marina ; Salt, David E. / MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants. In: The Plant Journal. 2009 ; Vol. 57, No. 6. pp. 1116-1127.
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