Mucosa-associated Faecalibacterium prausnitzii and Escherichia coli co-abundance can distinguish Irritable Bowel Syndrome and Inflammatory Bowel Disease phenotypes

Mireia Lopez-Siles, Margarita Martinez-Medina, David Busquets, Miriam Sabat-Mir, Sylvia H Duncan, Harry J Flint, Xavier Aldeguer, L Jesús Garcia-Gil

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

BACKGROUND: Crohn's disease (CD) and ulcerative colitis (UC) diagnosis requires comprehensive examination of the patient. Faecalibacterium prausnitzii and Escherichia coli have been reported as representatives of Inflammatory Bowel Disease (IBD) dysbiosis. The aim was to determine whether or not quantification of these species can be used as a complementary tool either for diagnostic or prognostic purposes.

METHODS: Mucosa-associated F. prausnitzii and E. coli abundance was determined in 28 controls (H), 45 CD, 28 UC patients and 10 irritable bowel syndrome (IBS) subjects by quantitative polymerase chain reaction (qPCR) and the F. prausnitzii-E. coli index (F-E index) was calculated. Species abundances were normalized to total bacteria and human cells. Data was analyzed taking into account patients' phenotype and most relevant clinical characteristics.

RESULTS: IBD patients had lower F. prausnitzii abundance than H and IBS (P<0.001). CD patients showed higher E. coli counts than H and UC patients (P<0.001). The F-E index discriminated between H, CD and UC patients, and even between disease phenotypes that are usually difficult to distinguish as ileal-CD (I-CD) from ileocolonic-CD and colonic-CD from extensive colitis. E. coli increased in active CD patients, and remission in I-CD patients was compromised by high abundance of this species. Treatment with anti-tumor necrosis factor (TNF) α diminished E. coli abundance in I-CD whereas none of the treatments counterbalanced F. prausnitzii depletion.

CONCLUSION: F. prausnitzii and E. coli are useful indicators to assist in IBD phenotype classification. The abundance of these species could also be used as a supporting prognostic tool in I-CD patients. Our data indicates that current medication does not restore the levels of these two species to those found in a healthy gut.

Original languageEnglish
Pages (from-to)464-475
Number of pages12
JournalInternational Journal of Medical Microbiology: IJMM
Volume304
Issue number3-4
Early online date20 Feb 2014
DOIs
Publication statusPublished - May 2014

Fingerprint

Irritable Bowel Syndrome
Inflammatory Bowel Diseases
Mucous Membrane
Crohn Disease
Escherichia coli
Phenotype
Ulcerative Colitis
Ileal Diseases
Dysbiosis
Faecalibacterium prausnitzii
Colonic Diseases
Colitis
Tumor Necrosis Factor-alpha
Bacteria
Polymerase Chain Reaction

Keywords

  • faecalibacterium prausnitzii
  • Escherichia coli
  • inflammatory bowel disease
  • irritable bowel syndrome
  • diagnostics
  • prognostics

Cite this

Mucosa-associated Faecalibacterium prausnitzii and Escherichia coli co-abundance can distinguish Irritable Bowel Syndrome and Inflammatory Bowel Disease phenotypes. / Lopez-Siles, Mireia; Martinez-Medina, Margarita; Busquets, David; Sabat-Mir, Miriam; Duncan, Sylvia H; Flint, Harry J; Aldeguer, Xavier; Garcia-Gil, L Jesús.

In: International Journal of Medical Microbiology: IJMM, Vol. 304, No. 3-4, 05.2014, p. 464-475.

Research output: Contribution to journalArticle

Lopez-Siles, Mireia ; Martinez-Medina, Margarita ; Busquets, David ; Sabat-Mir, Miriam ; Duncan, Sylvia H ; Flint, Harry J ; Aldeguer, Xavier ; Garcia-Gil, L Jesús. / Mucosa-associated Faecalibacterium prausnitzii and Escherichia coli co-abundance can distinguish Irritable Bowel Syndrome and Inflammatory Bowel Disease phenotypes. In: International Journal of Medical Microbiology: IJMM. 2014 ; Vol. 304, No. 3-4. pp. 464-475.
@article{acc0dc3a73314cdb96180c550c311f2d,
title = "Mucosa-associated Faecalibacterium prausnitzii and Escherichia coli co-abundance can distinguish Irritable Bowel Syndrome and Inflammatory Bowel Disease phenotypes",
abstract = "BACKGROUND: Crohn's disease (CD) and ulcerative colitis (UC) diagnosis requires comprehensive examination of the patient. Faecalibacterium prausnitzii and Escherichia coli have been reported as representatives of Inflammatory Bowel Disease (IBD) dysbiosis. The aim was to determine whether or not quantification of these species can be used as a complementary tool either for diagnostic or prognostic purposes.METHODS: Mucosa-associated F. prausnitzii and E. coli abundance was determined in 28 controls (H), 45 CD, 28 UC patients and 10 irritable bowel syndrome (IBS) subjects by quantitative polymerase chain reaction (qPCR) and the F. prausnitzii-E. coli index (F-E index) was calculated. Species abundances were normalized to total bacteria and human cells. Data was analyzed taking into account patients' phenotype and most relevant clinical characteristics.RESULTS: IBD patients had lower F. prausnitzii abundance than H and IBS (P<0.001). CD patients showed higher E. coli counts than H and UC patients (P<0.001). The F-E index discriminated between H, CD and UC patients, and even between disease phenotypes that are usually difficult to distinguish as ileal-CD (I-CD) from ileocolonic-CD and colonic-CD from extensive colitis. E. coli increased in active CD patients, and remission in I-CD patients was compromised by high abundance of this species. Treatment with anti-tumor necrosis factor (TNF) α diminished E. coli abundance in I-CD whereas none of the treatments counterbalanced F. prausnitzii depletion.CONCLUSION: F. prausnitzii and E. coli are useful indicators to assist in IBD phenotype classification. The abundance of these species could also be used as a supporting prognostic tool in I-CD patients. Our data indicates that current medication does not restore the levels of these two species to those found in a healthy gut.",
keywords = "faecalibacterium prausnitzii, Escherichia coli, inflammatory bowel disease, irritable bowel syndrome, diagnostics, prognostics",
author = "Mireia Lopez-Siles and Margarita Martinez-Medina and David Busquets and Miriam Sabat-Mir and Duncan, {Sylvia H} and Flint, {Harry J} and Xavier Aldeguer and Garcia-Gil, {L Jes{\'u}s}",
note = "Copyright {\circledC} 2014 Elsevier GmbH. All rights reserved. This work was partially funded by the Spanish Ministry of Education and Science through project SAF2010-15896. Mireia Lopez-Siles was recipient of an FI grant from the Generalitat de Catalunya (2010FI_B2 00135), which receives support from the European Union Commissionate. Prof. Harry J. Flint and Dr. Sylvia H. Duncan acknowledge support from the Scottish Government Food, Land and People programme. We thank Ms. Nat{\`a}lia Adell from the Serveis T{\`e}cnics de Recerca for statistical assistance. We are grateful to Dr. Laia Calv{\'o} (Research Unit, Institut d’Assist{\`e}ncia Sanit{\`a}ria, Salt, Spain) for her assistance in qPCR design and to Dr. Rosalia Trias (Universitat de Girona, Spain), who critically revised the manuscript. We appreciate the generosity of the patients who freely gave their time and samples to make this study possible, and the theatre staff of all centers for their dedication and careful sample collection.",
year = "2014",
month = "5",
doi = "10.1016/j.ijmm.2014.02.009",
language = "English",
volume = "304",
pages = "464--475",
journal = "International Journal of Medical Microbiology: IJMM",
issn = "1438-4221",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "3-4",

}

TY - JOUR

T1 - Mucosa-associated Faecalibacterium prausnitzii and Escherichia coli co-abundance can distinguish Irritable Bowel Syndrome and Inflammatory Bowel Disease phenotypes

AU - Lopez-Siles, Mireia

AU - Martinez-Medina, Margarita

AU - Busquets, David

AU - Sabat-Mir, Miriam

AU - Duncan, Sylvia H

AU - Flint, Harry J

AU - Aldeguer, Xavier

AU - Garcia-Gil, L Jesús

N1 - Copyright © 2014 Elsevier GmbH. All rights reserved. This work was partially funded by the Spanish Ministry of Education and Science through project SAF2010-15896. Mireia Lopez-Siles was recipient of an FI grant from the Generalitat de Catalunya (2010FI_B2 00135), which receives support from the European Union Commissionate. Prof. Harry J. Flint and Dr. Sylvia H. Duncan acknowledge support from the Scottish Government Food, Land and People programme. We thank Ms. Natàlia Adell from the Serveis Tècnics de Recerca for statistical assistance. We are grateful to Dr. Laia Calvó (Research Unit, Institut d’Assistència Sanitària, Salt, Spain) for her assistance in qPCR design and to Dr. Rosalia Trias (Universitat de Girona, Spain), who critically revised the manuscript. We appreciate the generosity of the patients who freely gave their time and samples to make this study possible, and the theatre staff of all centers for their dedication and careful sample collection.

PY - 2014/5

Y1 - 2014/5

N2 - BACKGROUND: Crohn's disease (CD) and ulcerative colitis (UC) diagnosis requires comprehensive examination of the patient. Faecalibacterium prausnitzii and Escherichia coli have been reported as representatives of Inflammatory Bowel Disease (IBD) dysbiosis. The aim was to determine whether or not quantification of these species can be used as a complementary tool either for diagnostic or prognostic purposes.METHODS: Mucosa-associated F. prausnitzii and E. coli abundance was determined in 28 controls (H), 45 CD, 28 UC patients and 10 irritable bowel syndrome (IBS) subjects by quantitative polymerase chain reaction (qPCR) and the F. prausnitzii-E. coli index (F-E index) was calculated. Species abundances were normalized to total bacteria and human cells. Data was analyzed taking into account patients' phenotype and most relevant clinical characteristics.RESULTS: IBD patients had lower F. prausnitzii abundance than H and IBS (P<0.001). CD patients showed higher E. coli counts than H and UC patients (P<0.001). The F-E index discriminated between H, CD and UC patients, and even between disease phenotypes that are usually difficult to distinguish as ileal-CD (I-CD) from ileocolonic-CD and colonic-CD from extensive colitis. E. coli increased in active CD patients, and remission in I-CD patients was compromised by high abundance of this species. Treatment with anti-tumor necrosis factor (TNF) α diminished E. coli abundance in I-CD whereas none of the treatments counterbalanced F. prausnitzii depletion.CONCLUSION: F. prausnitzii and E. coli are useful indicators to assist in IBD phenotype classification. The abundance of these species could also be used as a supporting prognostic tool in I-CD patients. Our data indicates that current medication does not restore the levels of these two species to those found in a healthy gut.

AB - BACKGROUND: Crohn's disease (CD) and ulcerative colitis (UC) diagnosis requires comprehensive examination of the patient. Faecalibacterium prausnitzii and Escherichia coli have been reported as representatives of Inflammatory Bowel Disease (IBD) dysbiosis. The aim was to determine whether or not quantification of these species can be used as a complementary tool either for diagnostic or prognostic purposes.METHODS: Mucosa-associated F. prausnitzii and E. coli abundance was determined in 28 controls (H), 45 CD, 28 UC patients and 10 irritable bowel syndrome (IBS) subjects by quantitative polymerase chain reaction (qPCR) and the F. prausnitzii-E. coli index (F-E index) was calculated. Species abundances were normalized to total bacteria and human cells. Data was analyzed taking into account patients' phenotype and most relevant clinical characteristics.RESULTS: IBD patients had lower F. prausnitzii abundance than H and IBS (P<0.001). CD patients showed higher E. coli counts than H and UC patients (P<0.001). The F-E index discriminated between H, CD and UC patients, and even between disease phenotypes that are usually difficult to distinguish as ileal-CD (I-CD) from ileocolonic-CD and colonic-CD from extensive colitis. E. coli increased in active CD patients, and remission in I-CD patients was compromised by high abundance of this species. Treatment with anti-tumor necrosis factor (TNF) α diminished E. coli abundance in I-CD whereas none of the treatments counterbalanced F. prausnitzii depletion.CONCLUSION: F. prausnitzii and E. coli are useful indicators to assist in IBD phenotype classification. The abundance of these species could also be used as a supporting prognostic tool in I-CD patients. Our data indicates that current medication does not restore the levels of these two species to those found in a healthy gut.

KW - faecalibacterium prausnitzii

KW - Escherichia coli

KW - inflammatory bowel disease

KW - irritable bowel syndrome

KW - diagnostics

KW - prognostics

U2 - 10.1016/j.ijmm.2014.02.009

DO - 10.1016/j.ijmm.2014.02.009

M3 - Article

VL - 304

SP - 464

EP - 475

JO - International Journal of Medical Microbiology: IJMM

JF - International Journal of Medical Microbiology: IJMM

SN - 1438-4221

IS - 3-4

ER -