Mucus: aiding elasmobranch conservation through non-invasive genetic sampling

Lilian Lieber, Simon Berrow, Emmett Johnston, Graham Hall, Jackie Hall, Chrysoula Gubili, David W. Sims, Catherine S. Jones, Leslie R. Noble*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)
8 Downloads (Pure)

Abstract

Large-scale genetic sampling by non-invasive methods is of vital importance for the conservation of vulnerable or elusive species. In the marine environment, non-invasive genetic sampling can provide a powerful alternative to conventional biopsies. We designed and implemented mucus swabbing for a free-ranging elasmobranch, thereby demonstrating the utility of this method in the field. We report the first attempt at mucus collection from 30 plankton-feeding basking sharks Cetorhinus maximus from 3 spatially distinct 'hotspots' in Irish waters. C. maximus DNA was successfully extracted and verified using DNA barcoding of the mitochondrial DNA cytochrome c oxidase 1 gene (99% sequence similarity) and basking shark species-specific multiplex PCRs derived from the nuclear ribosomal internal transcribed spacer 2 locus. Mitochondrial control region sequencing (1086 bp) showed that Irish samples were dominated by 2 haplotypes previously found to be globally distributed. Additionally, 1 novel haplotype was defined from western County Kerry. On-going genetic tagging will eventually provide more accurate estimates of global basking shark population structuring, abundance and behavioural ecology.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalEndangered Species Research
Volume21
Issue number3
Early online date6 Sep 2013
DOIs
Publication statusPublished - 2013

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Keywords

  • Basking shark
  • Cetorhinus maximus
  • Elasmobranchs
  • Genetic monitoring
  • Mucus swabs
  • Non-invasive sampling

ASJC Scopus subject areas

  • Nature and Landscape Conservation
  • Ecology

Cite this

Lieber, L., Berrow, S., Johnston, E., Hall, G., Hall, J., Gubili, C., ... Noble, L. R. (2013). Mucus: aiding elasmobranch conservation through non-invasive genetic sampling. Endangered Species Research, 21(3), 215-222. https://doi.org/10.3354/esr00524