Mug20, a novel protein associated with linear elements in fission yeast meiosis

Anna Estreicher, Alexander Lorenz, Josef Loidl

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the fission yeast, Schizosaccharomyces pombe, homologous chromosomes efficiently pair and recombine during meiotic prophase without forming a canonical synaptonemal complex (SC). Instead, it features simpler filamentous structures, the so-called linear elements (LinEs), which bear some resemblance to the axial/lateral element subunits of the SC. LinEs are required for wild-type recombination frequency. Here, we recognized Mug20, the product of a meiotically upregulated gene, as a LinE-associated protein. GFP-tagged Mug20 and anti-Mug20 antibody co-localized completely with Rec10, one of the major constituents of LinEs. In the absence of Mug20, LinEs failed to elongate beyond their initial state of nuclear dots. Foci of recombination protein Rad51 and genetic recombination were reduced. Since meiotic DNA double-strand breaks (DSBs), which initiate recombination, are induced at sites of preformed LinEs, we suggest that reduced recombination is a consequence of incomplete LinE extension. Therefore, we propose that Mug20 is required to extend LinEs from their sites of origin and thereby to increase DSB proficient regions on chromosomes.
Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalCurrent Genetics
Volume58
Issue number2
Early online date24 Feb 2012
DOIs
Publication statusPublished - 1 Apr 2012

Fingerprint

Schizosaccharomyces
Meiosis
Genetic Recombination
Synaptonemal Complex
Proteins
Chromosomes
Prophase
Double-Stranded DNA Breaks
Anti-Idiotypic Antibodies
Genes

Keywords

  • meiosis
  • chromosome pairing
  • synaptonemal complex
  • recombination

Cite this

Mug20, a novel protein associated with linear elements in fission yeast meiosis. / Estreicher, Anna; Lorenz, Alexander; Loidl, Josef.

In: Current Genetics, Vol. 58, No. 2, 01.04.2012, p. 119-127.

Research output: Contribution to journalArticle

Estreicher, Anna ; Lorenz, Alexander ; Loidl, Josef. / Mug20, a novel protein associated with linear elements in fission yeast meiosis. In: Current Genetics. 2012 ; Vol. 58, No. 2. pp. 119-127.
@article{2120ed239bd544a9abe237e4d1909f93,
title = "Mug20, a novel protein associated with linear elements in fission yeast meiosis",
abstract = "In the fission yeast, Schizosaccharomyces pombe, homologous chromosomes efficiently pair and recombine during meiotic prophase without forming a canonical synaptonemal complex (SC). Instead, it features simpler filamentous structures, the so-called linear elements (LinEs), which bear some resemblance to the axial/lateral element subunits of the SC. LinEs are required for wild-type recombination frequency. Here, we recognized Mug20, the product of a meiotically upregulated gene, as a LinE-associated protein. GFP-tagged Mug20 and anti-Mug20 antibody co-localized completely with Rec10, one of the major constituents of LinEs. In the absence of Mug20, LinEs failed to elongate beyond their initial state of nuclear dots. Foci of recombination protein Rad51 and genetic recombination were reduced. Since meiotic DNA double-strand breaks (DSBs), which initiate recombination, are induced at sites of preformed LinEs, we suggest that reduced recombination is a consequence of incomplete LinE extension. Therefore, we propose that Mug20 is required to extend LinEs from their sites of origin and thereby to increase DSB proficient regions on chromosomes.",
keywords = "meiosis, chromosome pairing, synaptonemal complex, recombination",
author = "Anna Estreicher and Alexander Lorenz and Josef Loidl",
year = "2012",
month = "4",
day = "1",
doi = "10.1007/s00294-012-0369-3",
language = "English",
volume = "58",
pages = "119--127",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Mug20, a novel protein associated with linear elements in fission yeast meiosis

AU - Estreicher, Anna

AU - Lorenz, Alexander

AU - Loidl, Josef

PY - 2012/4/1

Y1 - 2012/4/1

N2 - In the fission yeast, Schizosaccharomyces pombe, homologous chromosomes efficiently pair and recombine during meiotic prophase without forming a canonical synaptonemal complex (SC). Instead, it features simpler filamentous structures, the so-called linear elements (LinEs), which bear some resemblance to the axial/lateral element subunits of the SC. LinEs are required for wild-type recombination frequency. Here, we recognized Mug20, the product of a meiotically upregulated gene, as a LinE-associated protein. GFP-tagged Mug20 and anti-Mug20 antibody co-localized completely with Rec10, one of the major constituents of LinEs. In the absence of Mug20, LinEs failed to elongate beyond their initial state of nuclear dots. Foci of recombination protein Rad51 and genetic recombination were reduced. Since meiotic DNA double-strand breaks (DSBs), which initiate recombination, are induced at sites of preformed LinEs, we suggest that reduced recombination is a consequence of incomplete LinE extension. Therefore, we propose that Mug20 is required to extend LinEs from their sites of origin and thereby to increase DSB proficient regions on chromosomes.

AB - In the fission yeast, Schizosaccharomyces pombe, homologous chromosomes efficiently pair and recombine during meiotic prophase without forming a canonical synaptonemal complex (SC). Instead, it features simpler filamentous structures, the so-called linear elements (LinEs), which bear some resemblance to the axial/lateral element subunits of the SC. LinEs are required for wild-type recombination frequency. Here, we recognized Mug20, the product of a meiotically upregulated gene, as a LinE-associated protein. GFP-tagged Mug20 and anti-Mug20 antibody co-localized completely with Rec10, one of the major constituents of LinEs. In the absence of Mug20, LinEs failed to elongate beyond their initial state of nuclear dots. Foci of recombination protein Rad51 and genetic recombination were reduced. Since meiotic DNA double-strand breaks (DSBs), which initiate recombination, are induced at sites of preformed LinEs, we suggest that reduced recombination is a consequence of incomplete LinE extension. Therefore, we propose that Mug20 is required to extend LinEs from their sites of origin and thereby to increase DSB proficient regions on chromosomes.

KW - meiosis

KW - chromosome pairing

KW - synaptonemal complex

KW - recombination

UR - http://www.scopus.com/inward/record.url?scp=84860311075&partnerID=8YFLogxK

U2 - 10.1007/s00294-012-0369-3

DO - 10.1007/s00294-012-0369-3

M3 - Article

AN - SCOPUS:84860311075

VL - 58

SP - 119

EP - 127

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 2

ER -