Multi-mycotoxin exposure assessment in UK children using urinary biomarkers - a pilot survey

Silvia W Gratz; Valerie Currie; Gary Duncan; Diane Jackson

doi:10.1021/acs.jafc.9b03964

Multi-mycotoxin exposure assessment in UK children using urinary biomarkers - a pilot survey

Silvia W Gratz, Valerie Currie, Gary Duncan, Diane Jackson

The Rowett Institute of Nutrition and Health

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Abstract

Cereal foods are commonly contaminated with multiple mycotoxins resulting in frequent human mycotoxin exposure. Children are at risk of high-level exposure because of their high cereal intake relative to body weight. Hence, this study aims to assess multimycotoxin exposure in UK children using urinary biomarkers. Spot urines (n = 21) were analyzed for multimycotoxins (deoxynivalenol, DON; nivalenol, NW; ochratoxin A, OTA; zearalenone, ZEN; alpha-zearalenol, alpha-ZEL; beta-zearalenol, beta-ZEL; T-2 toxin, T-2; HT-2 toxin, HT-2; and aflatoxin B-1, and M-1, AFB(1), AFM(1)) using liquid chromatography-coupled tandem mass spectrometry. Urine samples frequently contained DON (13.10 +/- 12.69 ng/mL), NW (0.36 +/- 0.16 ng/mL), OTA (0.05 +/- 0.02 ng/mL), and ZEN (0.09 +/- 0.07 ng/mL). Some samples (1-3) contained T-2, HT-2, alpha-ZEL, and beta-ZEL but not aflatoxins. Dietary mycotoxin estimation showed that children were frequently exposed to levels exceeding the tolerable daily intake (52 and 95% of cases for DON and OTA). This demonstrates that UK children are exposed to multiple mycotoxins through their habitual diet.

Original language	English
Pages (from-to)	351-357
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	68
Issue number	1
Early online date	12 Dec 2019
DOIs	https://doi.org/10.1021/acs.jafc.9b03964
Publication status	Published - 2020

Keywords

trichothecenes
deoxynivalenol
ochratoxin
zearalenone
tolerable daily intake
co-exposure
diet
POPULATION
RISK
MYCOTOXIN EXPOSURE
FUSARIUM
OCHRATOXIN-A
INFANTS
COMPLEMENTARY FOODS
DEOXYNIVALENOL EXPOSURE
YOUNG-CHILDREN
CONSUMPTION

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Gratz_etal_acs_jafc_Multi_mycotoxin_exposure_AAM
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.9b03964
Accepted author manuscript, 305 KBLicence: Unspecified

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title = "Multi-mycotoxin exposure assessment in UK children using urinary biomarkers - a pilot survey",

abstract = "Cereal foods are commonly contaminated with multiple mycotoxins resulting in frequent human mycotoxin exposure. Children are at risk of high-level exposure because of their high cereal intake relative to body weight. Hence, this study aims to assess multimycotoxin exposure in UK children using urinary biomarkers. Spot urines (n = 21) were analyzed for multimycotoxins (deoxynivalenol, DON; nivalenol, NW; ochratoxin A, OTA; zearalenone, ZEN; alpha-zearalenol, alpha-ZEL; beta-zearalenol, beta-ZEL; T-2 toxin, T-2; HT-2 toxin, HT-2; and aflatoxin B-1, and M-1, AFB(1), AFM(1)) using liquid chromatography-coupled tandem mass spectrometry. Urine samples frequently contained DON (13.10 +/- 12.69 ng/mL), NW (0.36 +/- 0.16 ng/mL), OTA (0.05 +/- 0.02 ng/mL), and ZEN (0.09 +/- 0.07 ng/mL). Some samples (1-3) contained T-2, HT-2, alpha-ZEL, and beta-ZEL but not aflatoxins. Dietary mycotoxin estimation showed that children were frequently exposed to levels exceeding the tolerable daily intake (52 and 95% of cases for DON and OTA). This demonstrates that UK children are exposed to multiple mycotoxins through their habitual diet.",

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author = "Gratz, {Silvia W} and Valerie Currie and Gary Duncan and Diane Jackson",

year = "2020",

doi = "10.1021/acs.jafc.9b03964",

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AB - Cereal foods are commonly contaminated with multiple mycotoxins resulting in frequent human mycotoxin exposure. Children are at risk of high-level exposure because of their high cereal intake relative to body weight. Hence, this study aims to assess multimycotoxin exposure in UK children using urinary biomarkers. Spot urines (n = 21) were analyzed for multimycotoxins (deoxynivalenol, DON; nivalenol, NW; ochratoxin A, OTA; zearalenone, ZEN; alpha-zearalenol, alpha-ZEL; beta-zearalenol, beta-ZEL; T-2 toxin, T-2; HT-2 toxin, HT-2; and aflatoxin B-1, and M-1, AFB(1), AFM(1)) using liquid chromatography-coupled tandem mass spectrometry. Urine samples frequently contained DON (13.10 +/- 12.69 ng/mL), NW (0.36 +/- 0.16 ng/mL), OTA (0.05 +/- 0.02 ng/mL), and ZEN (0.09 +/- 0.07 ng/mL). Some samples (1-3) contained T-2, HT-2, alpha-ZEL, and beta-ZEL but not aflatoxins. Dietary mycotoxin estimation showed that children were frequently exposed to levels exceeding the tolerable daily intake (52 and 95% of cases for DON and OTA). This demonstrates that UK children are exposed to multiple mycotoxins through their habitual diet.

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KW - diet

KW - POPULATION

KW - RISK

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KW - INFANTS

KW - COMPLEMENTARY FOODS

KW - DEOXYNIVALENOL EXPOSURE

KW - YOUNG-CHILDREN

KW - CONSUMPTION

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SN - 0021-8561

VL - 68

SP - 351

EP - 357

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 1

ER -

Multi-mycotoxin exposure assessment in UK children using urinary biomarkers - a pilot survey

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Keywords

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