Multilocus Sequence Typing for Differentiation of Strains of Candida tropicalis

Arianna Tavanti, Amanda Denise Davidson, E. Johnson, M. C. Maiden, Duncan James Shaw, Neil Andrew Robert Gow, Frank Christopher Odds

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A system is described for typing isolates of the pathogenic fungus Candida tropicalis, based on sequence polymorphisms in fragments of six genes: ICL1, MDR1, SAPT2, SAPT4, XYR1, and ZWF1a. The system differentiated 87 diploid sequence types (DSTs) among a total of 106 isolates tested or 80 DSTs among 88 isolates from unique sources. Replicate isolates from the same source clustered together with high statistical similarity, with the exception of one isolate. However, a clade of very closely related isolates included replicate isolates from three different patients, as well as single isolates from eight other patients. This clade, provisionally designated clade 1, was one of three clusters of isolates with high statistical similarity. Five of six isolates in one cluster that may acquire clade status were resistant to flucytosine. This study adds C tropicalis to Candida albicans and Candida glabrata as Candida species for which a multilocus sequence typing (MLST) system has been set up. The C tropicalis MLST database can be accessed at http://pubmist.org/ctropicalis/.

Original languageEnglish
Pages (from-to)5593-5600
Number of pages7
JournalJournal of Clinical Microbiology
Volume43
Issue number11
DOIs
Publication statusPublished - Nov 2005

Keywords

  • ALBICANS
  • SYSTEM

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