Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus

Judith Margaret Bain, Arianna Tavanti, Amanda Denise Davidson, M. D. Jacobsen, D. Shaw, N. A. R. Gow, Frank Christopher Odds

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.

Original languageEnglish
Pages (from-to)1469-1477
Number of pages9
JournalJournal of Clinical Microbiology
Volume45
Issue number5
Early online date21 Mar 2007
DOIs
Publication statusPublished - May 2007

Keywords

  • fragment-length-polymorphism
  • polymerase chain-reaction
  • molecular epidemiology
  • random amplification
  • population-structure
  • invasive aspergillosis
  • candida-albicans
  • DNA
  • strains
  • infection

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