Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus

Judith Margaret Bain; Arianna Tavanti; Amanda Denise Davidson; M. D. Jacobsen; D. Shaw; N. A. R. Gow; Frank Christopher Odds

doi:10.1128/JCM.00064-07

Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus

Judith Margaret Bain, Arianna Tavanti, Amanda Denise Davidson, M. D. Jacobsen, D. Shaw, N. A. R. Gow, Frank Christopher Odds

Research output: Contribution to journal › Article

121 Citations (Scopus)

Abstract

A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.

Original language	English
Pages (from-to)	1469-1477
Number of pages	9
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	5
Early online date	21 Mar 2007
DOIs	https://doi.org/10.1128/JCM.00064-07
Publication status	Published - May 2007

Keywords

fragment-length-polymorphism
polymerase chain-reaction
molecular epidemiology
random amplification
population-structure
invasive aspergillosis
candida-albicans
DNA
strains
infection

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10.1128/JCM.00064-07

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title = "Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus",

abstract = "A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.",

keywords = "fragment-length-polymorphism, polymerase chain-reaction, molecular epidemiology, random amplification, population-structure, invasive aspergillosis, candida-albicans, DNA, strains, infection",

author = "Bain, {Judith Margaret} and Arianna Tavanti and Davidson, {Amanda Denise} and Jacobsen, {M. D.} and D. Shaw and Gow, {N. A. R.} and Odds, {Frank Christopher}",

year = "2007",

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doi = "10.1128/JCM.00064-07",

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AU - Bain, Judith Margaret

AU - Tavanti, Arianna

AU - Davidson, Amanda Denise

AU - Jacobsen, M. D.

AU - Shaw, D.

AU - Gow, N. A. R.

AU - Odds, Frank Christopher

PY - 2007/5

Y1 - 2007/5

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AB - A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.

KW - fragment-length-polymorphism

KW - polymerase chain-reaction

KW - molecular epidemiology

KW - random amplification

KW - population-structure

KW - invasive aspergillosis

KW - candida-albicans

KW - DNA

KW - strains

KW - infection

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DO - 10.1128/JCM.00064-07

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EP - 1477

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus

Abstract

Keywords

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