Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria

Ovidiu Rotariu, Iain D Ogden, Marion MacRae, L. A. Udrea, Norval James Colin Strachan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show > 97% recovery of polydisperse magnetic particles (diameter range 1 to 8 mu m) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.

Original languageEnglish
Pages (from-to)2967-2977
Number of pages10
JournalPhysics in Medicine and Biology
Volume50
Issue number12
DOIs
Publication statusPublished - Jun 2005

Keywords

  • ESCHERICHIA-COLI O157

Cite this

Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria. / Rotariu, Ovidiu; Ogden, Iain D; MacRae, Marion; Udrea, L. A.; Strachan, Norval James Colin.

In: Physics in Medicine and Biology, Vol. 50, No. 12, 06.2005, p. 2967-2977.

Research output: Contribution to journalArticle

@article{0dddbee0ee4544bebec2cbcb94d8dd3d,
title = "Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria",
abstract = "The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show > 97{\%} recovery of polydisperse magnetic particles (diameter range 1 to 8 mu m) containing 29-33{\%} w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.",
keywords = "ESCHERICHIA-COLI O157",
author = "Ovidiu Rotariu and Ogden, {Iain D} and Marion MacRae and Udrea, {L. A.} and Strachan, {Norval James Colin}",
year = "2005",
month = "6",
doi = "10.1088/0031-9155/50/12/016",
language = "English",
volume = "50",
pages = "2967--2977",
journal = "Physics in Medicine and Biology",
issn = "0031-9155",
publisher = "IOP Publishing Ltd.",
number = "12",

}

TY - JOUR

T1 - Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria

AU - Rotariu, Ovidiu

AU - Ogden, Iain D

AU - MacRae, Marion

AU - Udrea, L. A.

AU - Strachan, Norval James Colin

PY - 2005/6

Y1 - 2005/6

N2 - The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show > 97% recovery of polydisperse magnetic particles (diameter range 1 to 8 mu m) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.

AB - The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show > 97% recovery of polydisperse magnetic particles (diameter range 1 to 8 mu m) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.

KW - ESCHERICHIA-COLI O157

U2 - 10.1088/0031-9155/50/12/016

DO - 10.1088/0031-9155/50/12/016

M3 - Article

VL - 50

SP - 2967

EP - 2977

JO - Physics in Medicine and Biology

JF - Physics in Medicine and Biology

SN - 0031-9155

IS - 12

ER -