Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria

Ovidiu Rotariu, Iain D Ogden, Marion MacRae, L. A. Udrea, Norval James Colin Strachan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show > 97% recovery of polydisperse magnetic particles (diameter range 1 to 8 mu m) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.

Original languageEnglish
Pages (from-to)2967-2977
Number of pages10
JournalPhysics in Medicine and Biology
Volume50
Issue number12
DOIs
Publication statusPublished - Jun 2005

Keywords

  • ESCHERICHIA-COLI O157

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