Multiple tissue transcriptomic responses to Piscirickettsia salmonis in Atlantic salmon (Salmo salar)

L. Tacchi, C.J. Secombes, J.E. Bron, J.B. Taggart, R. Bickerdike, M.A. Adler, H. Takle, S.A.M. Martin

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The bacterium Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia (SRS), a severe disease that causes major economic losses to the Atlantic salmon aquaculture industry every year. Little is known about the infective strategy of P. salmonis, which is able to infect, survive within, and replicate inside salmonid macrophages as an intracellular parasite. Similarly there is little knowledge concerning the fish host's response to invasion by this pathogen. We have examined the transcriptional response of postsmolt Atlantic salmon (Salmo salar) to P. salmonis at 48 h following infection in three tissues, liver, head kidney, and muscle, using an Atlantic salmon oligonucleotide microarray (Salar_2, Agilent 4x44K). The infection led to a large alteration of transcriptional activity in all the tissues studied. In infected salmon 886, 207, and 153 transcripts were differentially expressed in liver, head kidney, and muscle, respectively. Assessment of enrichment for particular biological pathways by gene ontology analysis showed an upregulation of genes involved in oxidative and inflammatory responses in infected fish, indicative of the activation of the innate immune response. The downregulation of genes involved in the adaptive immune response, G protein signaling pathway, and apoptotic process in infected fish may be reflective of mechanisms used by P. salmonis to survive, replicate, and escape host defenses. There was also evidence of differential responses between studied tissues, with protein metabolism being decreased in muscle of infected fish and with a concomitant increase being shown in liver.
Original languageEnglish
Pages (from-to)1241-1254
Number of pages14
JournalPhysiological Genomics
Volume43
Issue number21
DOIs
Publication statusPublished - 1 Nov 2011

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Piscirickettsia
Salmo salar
Fishes
Head Kidney
Muscles
Liver
Aquaculture
Gene Ontology
Salmon
Adaptive Immunity
Infection
Oligonucleotide Array Sequence Analysis
GTP-Binding Proteins
Innate Immunity
Genes
Sepsis
Industry
Parasites
Up-Regulation
Down-Regulation

Keywords

  • salmonid rickettsial septicemia
  • microarray
  • transcriptome

Cite this

Multiple tissue transcriptomic responses to Piscirickettsia salmonis in Atlantic salmon (Salmo salar). / Tacchi, L.; Secombes, C.J.; Bron, J.E.; Taggart, J.B.; Bickerdike, R.; Adler, M.A.; Takle, H.; Martin, S.A.M.

In: Physiological Genomics, Vol. 43, No. 21, 01.11.2011, p. 1241-1254.

Research output: Contribution to journalArticle

Tacchi, L. ; Secombes, C.J. ; Bron, J.E. ; Taggart, J.B. ; Bickerdike, R. ; Adler, M.A. ; Takle, H. ; Martin, S.A.M. / Multiple tissue transcriptomic responses to Piscirickettsia salmonis in Atlantic salmon (Salmo salar). In: Physiological Genomics. 2011 ; Vol. 43, No. 21. pp. 1241-1254.
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