Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
|Title of host publication||Methods in Molecular Biology|
|Subtitle of host publication||Host-Fungus Interactions: Methods and Protocols|
|Editors||Alexandra Brand, Donna MacCallum|
|Place of Publication||London UK|
|Publisher||Springer Berlin / Heidelberg|
|Number of pages||15|
|Publication status||Published - 2012|
Gibson, J., Gow, N. A. R., & Wong, S. Y. C. (2012). Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. In A. Brand, & D. MacCallum (Eds.), Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols (Vol. 845, pp. 261-275). Springer Berlin / Heidelberg.