Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans

Joanne Gibson, Neil Andrew Robert Gow, Simon Y C Wong

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationHost-Fungus Interactions: Methods and Protocols
EditorsAlexandra Brand, Donna MacCallum
Place of PublicationLondon UK
PublisherSpringer Berlin / Heidelberg
Pages261-275
Number of pages15
Volume845
ISBN (Electronic)978-1-61779-539-8
ISBN (Print)978-1-61779-538-1
Publication statusPublished - 2012

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Gibson, J., Gow, N. A. R., & Wong, S. Y. C. (2012). Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. In A. Brand, & D. MacCallum (Eds.), Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols (Vol. 845, pp. 261-275). Springer Berlin / Heidelberg.