Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans

Joanne Gibson, Neil Andrew Robert Gow, Simon Y C Wong

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationHost-Fungus Interactions: Methods and Protocols
EditorsAlexandra Brand, Donna MacCallum
Place of PublicationLondon UK
PublisherSpringer Berlin / Heidelberg
Pages261-275
Number of pages15
Volume845
ISBN (Electronic)978-1-61779-539-8
ISBN (Print)978-1-61779-538-1
Publication statusPublished - 2012

Fingerprint

Candida albicans
Dendritic Cells
Bone Marrow
T-Lymphocytes
Cytokines
Mixed Lymphocyte Culture Test
Coculture Techniques

Cite this

Gibson, J., Gow, N. A. R., & Wong, S. Y. C. (2012). Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. In A. Brand, & D. MacCallum (Eds.), Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols (Vol. 845, pp. 261-275). London UK: Springer Berlin / Heidelberg.

Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. / Gibson, Joanne; Gow, Neil Andrew Robert; Wong, Simon Y C.

Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols . ed. / Alexandra Brand; Donna MacCallum. Vol. 845 London UK : Springer Berlin / Heidelberg, 2012. p. 261-275.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gibson, J, Gow, NAR & Wong, SYC 2012, Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. in A Brand & D MacCallum (eds), Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols . vol. 845, Springer Berlin / Heidelberg, London UK, pp. 261-275.
Gibson J, Gow NAR, Wong SYC. Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. In Brand A, MacCallum D, editors, Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols . Vol. 845. London UK: Springer Berlin / Heidelberg. 2012. p. 261-275
Gibson, Joanne ; Gow, Neil Andrew Robert ; Wong, Simon Y C. / Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans. Methods in Molecular Biology: Host-Fungus Interactions: Methods and Protocols . editor / Alexandra Brand ; Donna MacCallum. Vol. 845 London UK : Springer Berlin / Heidelberg, 2012. pp. 261-275
@inbook{e6e969d3f07f4e29a6e25c0c641a0758,
title = "Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans",
abstract = "Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.",
author = "Joanne Gibson and Gow, {Neil Andrew Robert} and Wong, {Simon Y C}",
year = "2012",
language = "English",
isbn = "978-1-61779-538-1",
volume = "845",
pages = "261--275",
editor = "Brand, {Alexandra } and Donna MacCallum",
booktitle = "Methods in Molecular Biology",
publisher = "Springer Berlin / Heidelberg",

}

TY - CHAP

T1 - Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans

AU - Gibson, Joanne

AU - Gow, Neil Andrew Robert

AU - Wong, Simon Y C

PY - 2012

Y1 - 2012

N2 - Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.

AB - Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.

M3 - Chapter

SN - 978-1-61779-538-1

VL - 845

SP - 261

EP - 275

BT - Methods in Molecular Biology

A2 - Brand, Alexandra

A2 - MacCallum, Donna

PB - Springer Berlin / Heidelberg

CY - London UK

ER -