Abstract
Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Subtitle of host publication | Host-Fungus Interactions: Methods and Protocols |
| Editors | Alexandra Brand, Donna MacCallum |
| Place of Publication | London UK |
| Publisher | Springer Berlin / Heidelberg |
| Pages | 261-275 |
| Number of pages | 15 |
| Volume | 845 |
| ISBN (Electronic) | 978-1-61779-539-8 |
| ISBN (Print) | 978-1-61779-538-1 |
| Publication status | Published - 2012 |