Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis

Sandra Stoppelkamp, Delyth M Reid, Joyce Yeoh, Julie Taylor, Emma J McKenzie, Gordon D Brown, Siamon Gordon, John V Forrester, Simon Y C Wong

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Abstract

Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.

Original languageEnglish
Pages (from-to)398-406
Number of pages9
JournalMolecular Immunology
Volume67
Issue number2 B
Early online date26 Jul 2015
DOIs
Publication statusPublished - Oct 2015

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Keywords

  • macrophage mannose receptor
  • dectin-1
  • mycobacteria
  • experimental autoimmune uveitis
  • pattern recognition receptor
  • adjuvant

Cite this

Stoppelkamp, S., Reid, D. M., Yeoh, J., Taylor, J., McKenzie, E. J., Brown, G. D., Gordon, S., Forrester, J. V., & Wong, S. Y. C. (2015). Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis. Molecular Immunology, 67(2 B), 398-406. https://doi.org/10.1016/j.molimm.2015.07.002