Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis

Sandra Stoppelkamp, Delyth M Reid, Joyce Yeoh, Julie Taylor, Emma J McKenzie, Gordon D Brown, Siamon Gordon, John V Forrester, Simon Y C Wong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.

Original languageEnglish
Pages (from-to)398-406
Number of pages9
JournalMolecular Immunology
Volume67
Issue number2 B
Early online date26 Jul 2015
DOIs
Publication statusPublished - Oct 2015

Fingerprint

Pattern Recognition Receptors
Freund's Adjuvant
Mycobacterium
Knockout Mice
C-Type Lectins
Autoimmune Experimental Encephalomyelitis
Adoptive Transfer
Antibodies
Myeloid Cells
Autoimmune Diseases
Immunization
Animal Models
Macrophages
dectin 1

Keywords

  • macrophage mannose receptor
  • dectin-1
  • mycobacteria
  • experimental autoimmune uveitis
  • pattern recognition receptor
  • adjuvant

Cite this

Stoppelkamp, S., Reid, D. M., Yeoh, J., Taylor, J., McKenzie, E. J., Brown, G. D., ... Wong, S. Y. C. (2015). Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis. Molecular Immunology, 67(2 B), 398-406. https://doi.org/10.1016/j.molimm.2015.07.002

Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis. / Stoppelkamp, Sandra; Reid, Delyth M; Yeoh, Joyce; Taylor, Julie; McKenzie, Emma J; Brown, Gordon D; Gordon, Siamon; Forrester, John V; Wong, Simon Y C.

In: Molecular Immunology, Vol. 67, No. 2 B, 10.2015, p. 398-406.

Research output: Contribution to journalArticle

Stoppelkamp, S, Reid, DM, Yeoh, J, Taylor, J, McKenzie, EJ, Brown, GD, Gordon, S, Forrester, JV & Wong, SYC 2015, 'Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis', Molecular Immunology, vol. 67, no. 2 B, pp. 398-406. https://doi.org/10.1016/j.molimm.2015.07.002
Stoppelkamp, Sandra ; Reid, Delyth M ; Yeoh, Joyce ; Taylor, Julie ; McKenzie, Emma J ; Brown, Gordon D ; Gordon, Siamon ; Forrester, John V ; Wong, Simon Y C. / Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis. In: Molecular Immunology. 2015 ; Vol. 67, No. 2 B. pp. 398-406.
@article{0c36d03523a54e19b97e9b2f75b458c9,
title = "Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis",
abstract = "Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.",
keywords = "macrophage mannose receptor, dectin-1, mycobacteria , experimental autoimmune uveitis, pattern recognition receptor, adjuvant",
author = "Sandra Stoppelkamp and Reid, {Delyth M} and Joyce Yeoh and Julie Taylor and McKenzie, {Emma J} and Brown, {Gordon D} and Siamon Gordon and Forrester, {John V} and Wong, {Simon Y C}",
note = "Copyright {\circledC} 2015. Published by Elsevier Ltd. This work was generously supported by the Wellcome Trust (GB), Cunningham Trust (JVF, DMR and SYCW), NHS Grampian Trust (SW), Tenovus Scotland (SW) and University of Aberdeen Development Trust (JVF). The funding bodies had no involvement in study design; in the collection, analysis and interpretation of data; in the writing of the report and in the decision to submit the article for publication. We also thank the technical staff at the Medical Research Facility (University of Aberdeen) for their help in maintaining the mice used in this study.",
year = "2015",
month = "10",
doi = "10.1016/j.molimm.2015.07.002",
language = "English",
volume = "67",
pages = "398--406",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "2 B",

}

TY - JOUR

T1 - Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis

AU - Stoppelkamp, Sandra

AU - Reid, Delyth M

AU - Yeoh, Joyce

AU - Taylor, Julie

AU - McKenzie, Emma J

AU - Brown, Gordon D

AU - Gordon, Siamon

AU - Forrester, John V

AU - Wong, Simon Y C

N1 - Copyright © 2015. Published by Elsevier Ltd. This work was generously supported by the Wellcome Trust (GB), Cunningham Trust (JVF, DMR and SYCW), NHS Grampian Trust (SW), Tenovus Scotland (SW) and University of Aberdeen Development Trust (JVF). The funding bodies had no involvement in study design; in the collection, analysis and interpretation of data; in the writing of the report and in the decision to submit the article for publication. We also thank the technical staff at the Medical Research Facility (University of Aberdeen) for their help in maintaining the mice used in this study.

PY - 2015/10

Y1 - 2015/10

N2 - Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.

AB - Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.

KW - macrophage mannose receptor

KW - dectin-1

KW - mycobacteria

KW - experimental autoimmune uveitis

KW - pattern recognition receptor

KW - adjuvant

U2 - 10.1016/j.molimm.2015.07.002

DO - 10.1016/j.molimm.2015.07.002

M3 - Article

VL - 67

SP - 398

EP - 406

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 2 B

ER -