Muscle Protein-Synthesis After Operation

Effects of Intravenous Nutrition

P ESSEN, M A MCNURLAN, T SONNENFELD, Eric Milne, E VINNARS, J WERNERMAN, P J GARLICK

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Objective: To assess the effect of an elective abdominal surgical operation (open cholecystectomy) on the rate of protein synthesis in skeletal muscle in humans.

    Design: Prospective random control trial.

    Setting: University hospital.

    Subjects: 17 Metabolically healthy patients who were to undergo elective open cholecystectomy.

    Interventions: Patients randomised to receive either saline alone (n = 8) or total parenteral nutrition (n = 9) for three days after operation. The rate of protein synthesis in muscle was calculated from the increase in enrichment of (1-C-13) leucine in protein after a flooding dose of (1-C-13) leucine.

    Results: Median (quartiles) rate of protein synthesis had decreased on the third postoperative day in the saline group by 49% (from 2.42 [2.03, 2.54] to 1.24 [0.99, 1.63]) and in the group that had received total parenteral nutrition by 54% (from 1.96 [1.90, 2.07] to 0.9110.79, 1.06]) (p < 0.01).

    Conclusions: The trauma associated with open cholecystectomy reduced the rate of protein synthesis in skeletal muscle by half in three days, and conventional total parenteral nutrition had no effect on these changes.

    Original languageEnglish
    Pages (from-to)195-200
    Number of pages6
    JournalEuropean Journal of Surgery
    Volume159
    Issue number4
    Publication statusPublished - Apr 1993

    Keywords

    • muscle
    • protein synthesis
    • stable isotope
    • surgery
    • parenteral nutrition
    • total parenteral-nutrition
    • skeletal-muscle
    • abdominal-surgery
    • nitrogen-balance
    • synthesis invivo
    • free glutamine
    • turnover
    • metabolism
    • <1-C-13>leucine
    • injection

    Cite this

    ESSEN, P., MCNURLAN, M. A., SONNENFELD, T., Milne, E., VINNARS, E., WERNERMAN, J., & GARLICK, P. J. (1993). Muscle Protein-Synthesis After Operation: Effects of Intravenous Nutrition. European Journal of Surgery, 159(4), 195-200.

    Muscle Protein-Synthesis After Operation : Effects of Intravenous Nutrition. / ESSEN, P ; MCNURLAN, M A ; SONNENFELD, T ; Milne, Eric; VINNARS, E ; WERNERMAN, J ; GARLICK, P J .

    In: European Journal of Surgery, Vol. 159, No. 4, 04.1993, p. 195-200.

    Research output: Contribution to journalArticle

    ESSEN, P, MCNURLAN, MA, SONNENFELD, T, Milne, E, VINNARS, E, WERNERMAN, J & GARLICK, PJ 1993, 'Muscle Protein-Synthesis After Operation: Effects of Intravenous Nutrition', European Journal of Surgery, vol. 159, no. 4, pp. 195-200.
    ESSEN P, MCNURLAN MA, SONNENFELD T, Milne E, VINNARS E, WERNERMAN J et al. Muscle Protein-Synthesis After Operation: Effects of Intravenous Nutrition. European Journal of Surgery. 1993 Apr;159(4):195-200.
    ESSEN, P ; MCNURLAN, M A ; SONNENFELD, T ; Milne, Eric ; VINNARS, E ; WERNERMAN, J ; GARLICK, P J . / Muscle Protein-Synthesis After Operation : Effects of Intravenous Nutrition. In: European Journal of Surgery. 1993 ; Vol. 159, No. 4. pp. 195-200.
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    title = "Muscle Protein-Synthesis After Operation: Effects of Intravenous Nutrition",
    abstract = "Objective: To assess the effect of an elective abdominal surgical operation (open cholecystectomy) on the rate of protein synthesis in skeletal muscle in humans.Design: Prospective random control trial.Setting: University hospital.Subjects: 17 Metabolically healthy patients who were to undergo elective open cholecystectomy.Interventions: Patients randomised to receive either saline alone (n = 8) or total parenteral nutrition (n = 9) for three days after operation. The rate of protein synthesis in muscle was calculated from the increase in enrichment of (1-C-13) leucine in protein after a flooding dose of (1-C-13) leucine.Results: Median (quartiles) rate of protein synthesis had decreased on the third postoperative day in the saline group by 49{\%} (from 2.42 [2.03, 2.54] to 1.24 [0.99, 1.63]) and in the group that had received total parenteral nutrition by 54{\%} (from 1.96 [1.90, 2.07] to 0.9110.79, 1.06]) (p < 0.01).Conclusions: The trauma associated with open cholecystectomy reduced the rate of protein synthesis in skeletal muscle by half in three days, and conventional total parenteral nutrition had no effect on these changes.",
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    T1 - Muscle Protein-Synthesis After Operation

    T2 - Effects of Intravenous Nutrition

    AU - ESSEN, P

    AU - MCNURLAN, M A

    AU - SONNENFELD, T

    AU - Milne, Eric

    AU - VINNARS, E

    AU - WERNERMAN, J

    AU - GARLICK, P J

    PY - 1993/4

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    N2 - Objective: To assess the effect of an elective abdominal surgical operation (open cholecystectomy) on the rate of protein synthesis in skeletal muscle in humans.Design: Prospective random control trial.Setting: University hospital.Subjects: 17 Metabolically healthy patients who were to undergo elective open cholecystectomy.Interventions: Patients randomised to receive either saline alone (n = 8) or total parenteral nutrition (n = 9) for three days after operation. The rate of protein synthesis in muscle was calculated from the increase in enrichment of (1-C-13) leucine in protein after a flooding dose of (1-C-13) leucine.Results: Median (quartiles) rate of protein synthesis had decreased on the third postoperative day in the saline group by 49% (from 2.42 [2.03, 2.54] to 1.24 [0.99, 1.63]) and in the group that had received total parenteral nutrition by 54% (from 1.96 [1.90, 2.07] to 0.9110.79, 1.06]) (p < 0.01).Conclusions: The trauma associated with open cholecystectomy reduced the rate of protein synthesis in skeletal muscle by half in three days, and conventional total parenteral nutrition had no effect on these changes.

    AB - Objective: To assess the effect of an elective abdominal surgical operation (open cholecystectomy) on the rate of protein synthesis in skeletal muscle in humans.Design: Prospective random control trial.Setting: University hospital.Subjects: 17 Metabolically healthy patients who were to undergo elective open cholecystectomy.Interventions: Patients randomised to receive either saline alone (n = 8) or total parenteral nutrition (n = 9) for three days after operation. The rate of protein synthesis in muscle was calculated from the increase in enrichment of (1-C-13) leucine in protein after a flooding dose of (1-C-13) leucine.Results: Median (quartiles) rate of protein synthesis had decreased on the third postoperative day in the saline group by 49% (from 2.42 [2.03, 2.54] to 1.24 [0.99, 1.63]) and in the group that had received total parenteral nutrition by 54% (from 1.96 [1.90, 2.07] to 0.9110.79, 1.06]) (p < 0.01).Conclusions: The trauma associated with open cholecystectomy reduced the rate of protein synthesis in skeletal muscle by half in three days, and conventional total parenteral nutrition had no effect on these changes.

    KW - muscle

    KW - protein synthesis

    KW - stable isotope

    KW - surgery

    KW - parenteral nutrition

    KW - total parenteral-nutrition

    KW - skeletal-muscle

    KW - abdominal-surgery

    KW - nitrogen-balance

    KW - synthesis invivo

    KW - free glutamine

    KW - turnover

    KW - metabolism

    KW - <1-C-13>leucine

    KW - injection

    M3 - Article

    VL - 159

    SP - 195

    EP - 200

    JO - European Journal of Surgery

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    SN - 1102-4151

    IS - 4

    ER -