Muscle protein synthesis in response to testosterone administration in wether lambs

Gerald Lobley, A Connell, Eric Milne, Vivien Buchan, Alexander Graham Calder, Susan Elizabeth Anderson, H Vint

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.
Original languageEnglish
Pages (from-to)691-704
Number of pages14
JournalBritish Journal of Nutrition
Volume64
Issue number3
Publication statusPublished - 1 Nov 1990

Fingerprint

Muscle Proteins
Testosterone
Proteins
RNA
DNA
Quadriceps Muscle
Isotopes
Nitrogen
Hormones
Biopsy

Keywords

  • Animals
  • Carbon Isotopes
  • DNA
  • Insulin
  • Leucine
  • Male
  • Methods
  • Muscle Proteins
  • Muscles
  • Nitrogen
  • RNA
  • Sheep
  • Testosterone

Cite this

Lobley, G., Connell, A., Milne, E., Buchan, V., Calder, A. G., Anderson, S. E., & Vint, H. (1990). Muscle protein synthesis in response to testosterone administration in wether lambs. British Journal of Nutrition, 64(3), 691-704.

Muscle protein synthesis in response to testosterone administration in wether lambs. / Lobley, Gerald; Connell, A; Milne, Eric; Buchan, Vivien; Calder, Alexander Graham; Anderson, Susan Elizabeth; Vint, H.

In: British Journal of Nutrition, Vol. 64, No. 3, 01.11.1990, p. 691-704.

Research output: Contribution to journalArticle

Lobley, G, Connell, A, Milne, E, Buchan, V, Calder, AG, Anderson, SE & Vint, H 1990, 'Muscle protein synthesis in response to testosterone administration in wether lambs' British Journal of Nutrition, vol. 64, no. 3, pp. 691-704.
Lobley G, Connell A, Milne E, Buchan V, Calder AG, Anderson SE et al. Muscle protein synthesis in response to testosterone administration in wether lambs. British Journal of Nutrition. 1990 Nov 1;64(3):691-704.
Lobley, Gerald ; Connell, A ; Milne, Eric ; Buchan, Vivien ; Calder, Alexander Graham ; Anderson, Susan Elizabeth ; Vint, H. / Muscle protein synthesis in response to testosterone administration in wether lambs. In: British Journal of Nutrition. 1990 ; Vol. 64, No. 3. pp. 691-704.
@article{f6b14a099d0f46e49b9ae56a3e90d237,
title = "Muscle protein synthesis in response to testosterone administration in wether lambs",
abstract = "A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96{\%} improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.",
keywords = "Animals, Carbon Isotopes, DNA, Insulin, Leucine, Male, Methods, Muscle Proteins, Muscles, Nitrogen, RNA, Sheep, Testosterone",
author = "Gerald Lobley and A Connell and Eric Milne and Vivien Buchan and Calder, {Alexander Graham} and Anderson, {Susan Elizabeth} and H Vint",
year = "1990",
month = "11",
day = "1",
language = "English",
volume = "64",
pages = "691--704",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge Univ. Press.",
number = "3",

}

TY - JOUR

T1 - Muscle protein synthesis in response to testosterone administration in wether lambs

AU - Lobley, Gerald

AU - Connell, A

AU - Milne, Eric

AU - Buchan, Vivien

AU - Calder, Alexander Graham

AU - Anderson, Susan Elizabeth

AU - Vint, H

PY - 1990/11/1

Y1 - 1990/11/1

N2 - A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.

AB - A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.

KW - Animals

KW - Carbon Isotopes

KW - DNA

KW - Insulin

KW - Leucine

KW - Male

KW - Methods

KW - Muscle Proteins

KW - Muscles

KW - Nitrogen

KW - RNA

KW - Sheep

KW - Testosterone

M3 - Article

VL - 64

SP - 691

EP - 704

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 3

ER -