A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.
|Number of pages||14|
|Journal||British Journal of Nutrition|
|Publication status||Published - 1 Nov 1990|
- Carbon Isotopes
- Muscle Proteins
Lobley, G., Connell, A., Milne, E., Buchan, V., Calder, A. G., Anderson, S. E., & Vint, H. (1990). Muscle protein synthesis in response to testosterone administration in wether lambs. British Journal of Nutrition, 64(3), 691-704.