Muscle protein synthesis in response to testosterone administration in wether lambs

Gerald Lobley, A Connell, Eric Milne, Vivien Buchan, Alexander Graham Calder, Susan Elizabeth Anderson, H Vint

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40-44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein:DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.
Original languageEnglish
Pages (from-to)691-704
Number of pages14
JournalBritish Journal of Nutrition
Issue number3
Publication statusPublished - 1 Nov 1990


  • Animals
  • Carbon Isotopes
  • DNA
  • Insulin
  • Leucine
  • Male
  • Methods
  • Muscle Proteins
  • Muscles
  • Nitrogen
  • RNA
  • Sheep
  • Testosterone


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