Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds

Brian M. Waters, Heng-Hsuan Chu, Raymond J. DiDonato, Louis A. Roberts, Robynn B. Eisley, Brett Lahner, David E. Salt, Elsbeth L. Walker

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Here, we describe two members of the Arabidopsis ( Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. beta-Glucuronidase reporter expression, driven by YSL1 and YSL3 promoters, reveals expression patterns of the genes in roots, leaves, and flowers. Expression was highest in senescing rosette leaves and cauline leaves. Whereas the single mutants ysl1 and ysl3 had no visible phenotypes, the ysl1ysl3 double mutant exhibited Fe deficiency symptoms, such as interveinal chlorosis. Leaf Fe concentrations are decreased in the double mutant, whereas manganese, zinc, and especially copper concentrations are elevated. In seeds of double-mutant plants, the concentrations of Fe, zinc, and copper are low. Mobilization of metals from leaves during senescence is impaired in the double mutant. In addition, the double mutant has reduced fertility due to defective anther and embryo development. The proposed physiological roles for YSL1 and YSL3 are in delivery of metal micronutrients to and from vascular tissues.

Original languageEnglish
Pages (from-to)1446-1458
Number of pages13
JournalPlant Physiology
Volume141
Issue number4
DOIs
Publication statusPublished - Aug 2006

Keywords

  • nicotianamine synthase genes
  • lycopersicon-esculentum mill
  • tomato mutant chloronerva
  • ferric-chelate reductase
  • iron uptake
  • yellow stripe1
  • heavy-metals
  • transporter
  • plants
  • thaliana

Cite this

Waters, B. M., Chu, H-H., DiDonato, R. J., Roberts, L. A., Eisley, R. B., Lahner, B., ... Walker, E. L. (2006). Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiology, 141(4), 1446-1458. https://doi.org/10.1104/pp.106.082586

Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. / Waters, Brian M.; Chu, Heng-Hsuan; DiDonato, Raymond J.; Roberts, Louis A.; Eisley, Robynn B.; Lahner, Brett; Salt, David E.; Walker, Elsbeth L.

In: Plant Physiology, Vol. 141, No. 4, 08.2006, p. 1446-1458.

Research output: Contribution to journalArticle

Waters, Brian M. ; Chu, Heng-Hsuan ; DiDonato, Raymond J. ; Roberts, Louis A. ; Eisley, Robynn B. ; Lahner, Brett ; Salt, David E. ; Walker, Elsbeth L. / Mutations in Arabidopsis Yellow Stripe-Like1 and Yellow Stripe-Like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. In: Plant Physiology. 2006 ; Vol. 141, No. 4. pp. 1446-1458.
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abstract = "Here, we describe two members of the Arabidopsis ( Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. beta-Glucuronidase reporter expression, driven by YSL1 and YSL3 promoters, reveals expression patterns of the genes in roots, leaves, and flowers. Expression was highest in senescing rosette leaves and cauline leaves. Whereas the single mutants ysl1 and ysl3 had no visible phenotypes, the ysl1ysl3 double mutant exhibited Fe deficiency symptoms, such as interveinal chlorosis. Leaf Fe concentrations are decreased in the double mutant, whereas manganese, zinc, and especially copper concentrations are elevated. In seeds of double-mutant plants, the concentrations of Fe, zinc, and copper are low. Mobilization of metals from leaves during senescence is impaired in the double mutant. In addition, the double mutant has reduced fertility due to defective anther and embryo development. The proposed physiological roles for YSL1 and YSL3 are in delivery of metal micronutrients to and from vascular tissues.",
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AU - Waters, Brian M.

AU - Chu, Heng-Hsuan

AU - DiDonato, Raymond J.

AU - Roberts, Louis A.

AU - Eisley, Robynn B.

AU - Lahner, Brett

AU - Salt, David E.

AU - Walker, Elsbeth L.

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N2 - Here, we describe two members of the Arabidopsis ( Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. beta-Glucuronidase reporter expression, driven by YSL1 and YSL3 promoters, reveals expression patterns of the genes in roots, leaves, and flowers. Expression was highest in senescing rosette leaves and cauline leaves. Whereas the single mutants ysl1 and ysl3 had no visible phenotypes, the ysl1ysl3 double mutant exhibited Fe deficiency symptoms, such as interveinal chlorosis. Leaf Fe concentrations are decreased in the double mutant, whereas manganese, zinc, and especially copper concentrations are elevated. In seeds of double-mutant plants, the concentrations of Fe, zinc, and copper are low. Mobilization of metals from leaves during senescence is impaired in the double mutant. In addition, the double mutant has reduced fertility due to defective anther and embryo development. The proposed physiological roles for YSL1 and YSL3 are in delivery of metal micronutrients to and from vascular tissues.

AB - Here, we describe two members of the Arabidopsis ( Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. beta-Glucuronidase reporter expression, driven by YSL1 and YSL3 promoters, reveals expression patterns of the genes in roots, leaves, and flowers. Expression was highest in senescing rosette leaves and cauline leaves. Whereas the single mutants ysl1 and ysl3 had no visible phenotypes, the ysl1ysl3 double mutant exhibited Fe deficiency symptoms, such as interveinal chlorosis. Leaf Fe concentrations are decreased in the double mutant, whereas manganese, zinc, and especially copper concentrations are elevated. In seeds of double-mutant plants, the concentrations of Fe, zinc, and copper are low. Mobilization of metals from leaves during senescence is impaired in the double mutant. In addition, the double mutant has reduced fertility due to defective anther and embryo development. The proposed physiological roles for YSL1 and YSL3 are in delivery of metal micronutrients to and from vascular tissues.

KW - nicotianamine synthase genes

KW - lycopersicon-esculentum mill

KW - tomato mutant chloronerva

KW - ferric-chelate reductase

KW - iron uptake

KW - yellow stripe1

KW - heavy-metals

KW - transporter

KW - plants

KW - thaliana

U2 - 10.1104/pp.106.082586

DO - 10.1104/pp.106.082586

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VL - 141

SP - 1446

EP - 1458

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -