Nasal and bronchial airway epithelial cell mediator release in children

Pringle Emily, Helen Richardson, David Miller, Daniella Maria Spiteri Cornish, Graham Stuart Devereux, Garry Michael Walsh, Stephen William Turner

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective
The present study was designed to test the hypothesis that airway epithelial cell (AEC) mediator release is similar in upper and lower airway AEC in children.
Methods
Nasal and bronchial AEC were collected by brushings from children scheduled for general anesthetic. AEC release of the following mediators was measured: interleukin (IL)-6, IL-8, Granulocyte Colony Stimulating Factor (G-CSF), regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), matrix metallo proteinase (MMP)-9, and tissue inhibitor of metalloproteinases (TIMP)-1.
Results
AEC were cultured in 34 children, mean age 7.3 years. Release of IL-6, IL-8, and G-CSF was significantly higher in nasal compared with bronchial AEC but nasal and bronchial AEC release of other mediators was not significantly different. Treatment of AEC with IL-1 ß and tumor necrosis factor-a increased secretion of all mediators. Release of IL-6 and GSCF remained higher in nasal AEC compared with bronchial AEC following stimulation.
Conclusions
In epidemiological studies, nasal AEC may be a useful surrogate for bronchial AEC for the study of RANTES, MCP-1, TIMP-1, and MMP-9 release in children but bronchial AEC will remain the gold standard. Pediatr Pulmonol. © 2012 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)1215-1225
Number of pages11
JournalPediatric Pulmonology
Volume47
Issue number12
Early online date28 Sep 2012
DOIs
Publication statusPublished - Dec 2012

Keywords

  • nasal epithelium
  • bronchial epithelium
  • paired comparison

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