Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

Bruno Silvester Lopes; Norval James Colin Strachan; Meenakshi Ramjee; Anne Patricia Thomson; Marion MacRae; Sophie Shaw; Ken J. Forbes

doi:10.3201/eid2507.181572

Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

Bruno Silvester Lopes, Norval James Colin Strachan, Meenakshi Ramjee, Anne Patricia Thomson, Marion MacRae, Sophie Shaw, Ken J. Forbes

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

2 Downloads (Pure)

Abstract

We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

Original language	English
Pages (from-to)	1320-1329
Number of pages	10
Journal	Emerging Infectious Diseases
Volume	25
Issue number	7
Early online date	29 May 2019
DOIs	https://doi.org/10.3201/eid2507.181572
Publication status	Published - Jul 2019

Keywords

ANTIMICROBIAL SUSCEPTIBILITY
MOLECULAR EPIDEMIOLOGY
ANTIBIOTIC-RESISTANCE
COMMUNITY
GENES
multidrug resistance
poultry
clones
bacteria
endemic
Scotland
United Kingdom
Campylobacter jejuni
antimicrobial drugs
antimicrobial resistance
host reservoirs
broiler industry
ST5136
antibiotics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3201/eid2507.181572Licence: CC BY

Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom
Emerging Infectious Diseases is an open access journal in the public domain. All content is freely available without charge to the user or his/her institution. In accordance with the Budapest Open Access Initiative definition of Open Access, users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. Because the journal is in the public domain, its usage policy also conforms to conditions set for by Creative Commons. https://creativecommons.org/licenses/by/4.0/
Final published version, 1.92 MBLicence: CC BY

Cite this

@article{a4503afbcb4846df91c46cce5289149c,

title = "Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom",

abstract = "We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.",

keywords = "ANTIMICROBIAL SUSCEPTIBILITY, MOLECULAR EPIDEMIOLOGY, ANTIBIOTIC-RESISTANCE, COMMUNITY, GENES, multidrug resistance, poultry, clones, bacteria, endemic, Scotland, United Kingdom, Campylobacter jejuni, antimicrobial drugs, antimicrobial resistance, host reservoirs, broiler industry, ST5136, antibiotics",

author = "Lopes, {Bruno Silvester} and Strachan, {Norval James Colin} and Meenakshi Ramjee and Thomson, {Anne Patricia} and Marion MacRae and Sophie Shaw and Forbes, {Ken J.}",

note = "We thank Food Standards Scotland (Contracts S14054, FSS00017) and the Scottish Government{\textquoteright}s Rural and Environment Science and Analytical Services (RG13588-10) for funding this work. Part of this work was presented at the Campylobacter, Helicobacter and Related Organisms (CHRO) 2017 Congress, Nantes, France. ",

year = "2019",

month = jul,

doi = "10.3201/eid2507.181572",

language = "English",

volume = "25",

pages = "1320--1329",

journal = "Emerging Infectious Diseases",

issn = "1080-6040",

publisher = "CENTERS DISEASE CONTROL",

number = "7",

}

TY - JOUR

T1 - Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

AU - Lopes, Bruno Silvester

AU - Strachan, Norval James Colin

AU - Ramjee, Meenakshi

AU - Thomson, Anne Patricia

AU - MacRae, Marion

AU - Shaw, Sophie

AU - Forbes, Ken J.

N1 - We thank Food Standards Scotland (Contracts S14054, FSS00017) and the Scottish Government’s Rural and Environment Science and Analytical Services (RG13588-10) for funding this work. Part of this work was presented at the Campylobacter, Helicobacter and Related Organisms (CHRO) 2017 Congress, Nantes, France.

PY - 2019/7

Y1 - 2019/7

N2 - We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

AB - We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

KW - ANTIMICROBIAL SUSCEPTIBILITY

KW - MOLECULAR EPIDEMIOLOGY

KW - ANTIBIOTIC-RESISTANCE

KW - COMMUNITY

KW - GENES

KW - multidrug resistance

KW - poultry

KW - clones

KW - bacteria

KW - endemic

KW - Scotland

KW - United Kingdom

KW - Campylobacter jejuni

KW - antimicrobial drugs

KW - antimicrobial resistance

KW - host reservoirs

KW - broiler industry

KW - ST5136

KW - antibiotics

UR - http://www.mendeley.com/research/nationwide-stepwise-emergence-evolution-multidrugresistant-campylobacter-jejuni-sequence-type-5136-u

UR - http://www.scopus.com/inward/record.url?scp=85069321686&partnerID=8YFLogxK

U2 - 10.3201/eid2507.181572

DO - 10.3201/eid2507.181572

M3 - Article

C2 - 31211671

VL - 25

SP - 1320

EP - 1329

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

SN - 1080-6040

IS - 7

ER -

Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Norval Strachan

Cite this

Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Profiles

Norval Strachan

Cite this