Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

Bruno Silvester Lopes, Norval James Colin Strachan, Meenakshi Ramjee, Anne Patricia Thomson, Marion MacRae, Sophie Shaw, Ken J. Forbes

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Abstract

We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.
Original languageEnglish
Pages (from-to)1320-1329
Number of pages10
JournalEmerging Infectious Diseases
Volume25
Issue number7
Early online date29 May 2019
DOIs
Publication statusPublished - Jul 2019

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Campylobacter jejuni
Fluoroquinolones
Aminoglycosides
Poultry
Campylobacter coli
Genes
Ampicillin Resistance
Tetracycline Resistance
Lactams
Scotland
Tetracycline
Genetic Promoter Regions
Birds
Industry
Genome
Pharmaceutical Preparations
United Kingdom

Keywords

  • ANTIMICROBIAL SUSCEPTIBILITY
  • MOLECULAR EPIDEMIOLOGY
  • ANTIBIOTIC-RESISTANCE
  • COMMUNITY
  • GENES
  • multidrug resistance
  • poultry
  • clones
  • bacteria
  • endemic
  • Scotland
  • United Kingdom
  • Campylobacter jejuni
  • antimicrobial drugs
  • antimicrobial resistance
  • host reservoirs
  • broiler industry
  • ST5136
  • antibiotics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom. / Lopes, Bruno Silvester; Strachan, Norval James Colin; Ramjee, Meenakshi; Thomson, Anne Patricia; MacRae, Marion; Shaw, Sophie; Forbes, Ken J.

In: Emerging Infectious Diseases, Vol. 25, No. 7, 07.2019, p. 1320-1329.

Research output: Contribution to journalArticle

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abstract = "We examined whole-genome–sequenced Campylobacter jejuni/coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G → T transversion in the −10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.",
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