Natural genetic transformation in the rumen bacterium Streptococcus bovis JB1

D K Mercer, C M Melville, K P Scott, H J Flint

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Natural transformation of Streptococcus bovis JB1 was demonstrated after development of competence in normal culture medium. Transformation efficiencies were not significantly increased when heat-inactivated horse serum was added to the medium before growth. This is the first time that a resident rumen bacterial species has been shown to be naturally transformable. Transformation allowed the acquisition of plasmids or integration of sequences into the chromosome. No transformation was observed in the presence of undiluted autoclaved or filter-sterilised ovine rumen fluid or filter-sterilised ovine saliva, suggesting that transformation in the ruminant digestive tract is a rare event, although transformation was observed in the presence of 1% and 0.5% filter-sterilised rumen fluid. The use of natural transformation of S. bovis should facilitate Further molecular biological studies on this species. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalFEMS Microbiology Letters
Volume179
Issue number2
Publication statusPublished - 15 Oct 1999

Keywords

  • transformation
  • rumen
  • gene transfer
  • Streptococcus
  • BUTYRIVIBRIO-FIBRISOLVENS
  • TETRACYCLINE RESISTANCE
  • PLASMID DNA
  • ELECTROPORATION
  • CLONING
  • VECTORS

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