Neutrophils produce IL-17A in a Dectin-1 and IL-23 dependent manner during invasive fungal infection

Jessica L Werner, Melissa A Gessner, Lauren M Lilly, Michael P Nelson, Allison E Metz, Dawn Horn, Chad W Dunaway, Jessy Deshane, David D Chaplin, Casey T Weaver, Gordon D Brown, Chad Steele

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117 Citations (Scopus)

Abstract

We have previously reported that compromised IL-17A production in the lungs increased susceptibility to infection with the invasive fungal pathogen Aspergillus fumigatus. Here, we show that culturing lung cells from A. fumigatus-challenged mice ex vivo demonstrated Dectin-1 dependent IL-17A production. In this system, neutralization of IL-23, but not IL-6, IL-1ß or IL-18, resulted in attenuated IL-17A production. Il23 mRNA expression was found to be lower in lung cells from A. fumigatus-challenged Dectin-1 deficient mice whereas bone marrow-derived dendritic cells from Dectin-1 deficient mice failed to produce IL-23 in response to A. fumigatus in vitro. Addition of recombinant IL-23 augmented IL-17A production by WT and Dectin-1 deficient lung cells, although the addition of IL-6 or IL-1ß did not augment the effect of IL-23. Intracellular cytokine staining of lung cells revealed lower CD11b+ IL-17A+ and Ly-6G+ IL-17A+ cells in A. fumigatus-challenged Dectin-1 deficient mice. Ly-6G+ neutrophils purified from the lungs of A. fumigatus-challenged Dectin-1 deficient mice displayed lower Il17a mRNA expression, but surprisingly had intact Rorc and Rora mRNA expression. We further demonstrate that Ly-6G+ neutrophils required the presence of myeloid cells for IL-17A production. Finally, upon in vitro stimulation with A. fumigatus, thioglycollate-elicited peritoneal neutrophils were positive for intracellular IL-17A expression and produced IL-17A in a Dectin-1 and IL-23 dependent manner. In summary, Dectin-1 dependent IL-17A production in the lungs during invasive fungal infection is mediated in part by CD11b+ Ly-6G+ neutrophils in an IL-23 dependent manner.
Original languageEnglish
Pages (from-to)3966-3977
Number of pages12
JournalInfection and Immunity
Volume79
Issue number10
Early online date1 Aug 2011
DOIs
Publication statusPublished - Oct 2011

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Werner, J. L., Gessner, M. A., Lilly, L. M., Nelson, M. P., Metz, A. E., Horn, D., ... Steele, C. (2011). Neutrophils produce IL-17A in a Dectin-1 and IL-23 dependent manner during invasive fungal infection. Infection and Immunity, 79(10), 3966-3977. https://doi.org/10.1128/IAI.05493-11