Niche-specific regulation of central metabolic pathways in a fungal pathogen

Caroline Barelle, Claire L Priest, Donna Margaret MacCallum, Neil Andrew Robert Gow, Frank Christopher Odds, Alistair James Petersen Brown

Research output: Contribution to journalArticle

194 Citations (Scopus)
4 Downloads (Pure)

Abstract

To establish an infection, the pathogen Candida albicans must assimilate carbon and grow in its mammalian host. This fungus assimilates six-carbon compounds via the glycolytic pathway, and two-carbon compounds via the glyoxylate cycle and gluconeogenesis. We address a paradox regarding the roles of these central metabolic pathways in C. albicans pathogenesis: the glyoxylate cycle is apparently required for virulence although glyoxylate cycle genes are repressed by glucose at concentrations present in the bloodstream. Using GFP fusions, we confirm that glyoxylate cycle and gluconeogenic genes in C. albicans are repressed by physiologically relevant concentrations of glucose, and show that these genes are inactive in the majority of fungal cells infecting the mouse kidney. However, these pathways are induced following phagocytosis by macrophages or neutrophils. In contrast, glycolytic genes are not induced following phagocytosis and are expressed in infected kidney. Mutations in all three pathways attenuate the virulence of this fungus, highlighting the importance of central carbon metabolism for the establishment of C. albicans infections. We conclude that C. albicans displays a metabolic program whereby the glyoxylate cycle and gluconeogenesis are activated early, when the pathogen is phagocytosed by host cells, while the subsequent progression of systemic disease is dependent upon glycolysis.
Original languageEnglish
Pages (from-to)961-971
Number of pages11
JournalCellular Microbiology
Volume8
Issue number6
Early online date13 Jan 2006
DOIs
Publication statusPublished - 1 Jun 2006

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Metabolic Networks and Pathways
Candida albicans
Carbon
Gluconeogenesis
Phagocytosis
Genes
Virulence
Fungi
Kidney
Glucose
Cytophagocytosis
Glycolysis
Infection
Disease Progression
Neutrophils
Macrophages
glyoxylic acid
Mutation

Keywords

  • Animals
  • Bacterial Proteins
  • Candida albicans
  • Candidiasis
  • Carbon
  • DNA, Fungal
  • Female
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Gluconeogenesis
  • Glucose
  • Glycolysis
  • Glyoxylates
  • Isocitrate Lyase
  • Kidney
  • Metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Phagocytosis
  • Phosphoenolpyruvate Carboxykinase (ATP)
  • Phosphofructokinase-2
  • Pyruvate Kinase
  • Virulence

Cite this

Niche-specific regulation of central metabolic pathways in a fungal pathogen. / Barelle, Caroline; Priest, Claire L; MacCallum, Donna Margaret; Gow, Neil Andrew Robert; Odds, Frank Christopher; Brown, Alistair James Petersen.

In: Cellular Microbiology, Vol. 8, No. 6, 01.06.2006, p. 961-971.

Research output: Contribution to journalArticle

Barelle, Caroline ; Priest, Claire L ; MacCallum, Donna Margaret ; Gow, Neil Andrew Robert ; Odds, Frank Christopher ; Brown, Alistair James Petersen. / Niche-specific regulation of central metabolic pathways in a fungal pathogen. In: Cellular Microbiology. 2006 ; Vol. 8, No. 6. pp. 961-971.
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