Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria

Marta A. Streminska, Heather Felgate, Gary Rowley, David J. Richardson, Elizabeth M. Baggs

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Here we provide the first demonstration of the potential for N2O production by soil-isolated nitrate-ammonifying bacteria under different C and N availabilities, building on characterizations informed from model strains. The potential for soil-isolated Bacillus sp. and Citrobacter sp. to reduce NO3-, and produce NH4+, NO2- and N2O was examined in batch and continuous (chemostat) cultures under different C-to-NO3- ratios, NO3--limiting (5 mM) and NO3--sufficient (22 mM) conditions. C-to-NO3- ratio had a major influence on the products of nitrate ammonification, with NO2-, rather than NH4+, being the major product at low C-to-NO3- ratios in batch cultures. N2O production was maximum and accompanied by high NO2- production under C-limitation/NO3-sufficiency conditions in chemostat cultures. In media with lower C-to-NO3-N ratios (5- and 10-to-1) up to 2.7% or 5.0% of NO3- was reduced to N2O by Bacillus sp. and Citrobacter sp., respectively, but these reduction efficiencies were only 0.1% or 0.7% at higher C-to-NO3- ratios (25- and 50-to-1). As the highest N2O production did not occur under the same C-to-NO3- conditions as highest NH4+ production we suggest that a re-evaluation may be necessary of the environmental conditions under which nitrate ammonification contributes to N2O emission from soil.

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalEnvironmental Microbiology Reports
Volume4
Issue number1
Early online date31 Oct 2011
DOIs
Publication statusPublished - Feb 2012

Cite this

Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria. / Streminska, Marta A.; Felgate, Heather; Rowley, Gary; Richardson, David J.; Baggs, Elizabeth M.

In: Environmental Microbiology Reports, Vol. 4, No. 1, 02.2012, p. 66-71.

Research output: Contribution to journalArticle

Streminska, MA, Felgate, H, Rowley, G, Richardson, DJ & Baggs, EM 2012, 'Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria', Environmental Microbiology Reports, vol. 4, no. 1, pp. 66-71. https://doi.org/10.1111/j.1758-2229.2011.00302.x
Streminska, Marta A. ; Felgate, Heather ; Rowley, Gary ; Richardson, David J. ; Baggs, Elizabeth M. / Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria. In: Environmental Microbiology Reports. 2012 ; Vol. 4, No. 1. pp. 66-71.
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