TY - JOUR
T1 - Nitroxide free radical clearance in the live rat monitored by radio- frequency CW-EPR and PEDRI
AU - Alecci, Marcello
AU - Seimenis, Ioannis
AU - McCallum, Stephen J.
AU - Lurie, David J.
AU - Foster, Margaret A.
N1 - M Alecci acknowledges the support of the European Union for two fellowships under the ‘Human Capital and Mobility’ (ERBCHBICT940953) and ‘Training and Mobility of Researchers’ (ERBFMBICT960973) programmes. S J McCallum was supported by a grant from the Leverhulme Trust.
PY - 1998/7/30
Y1 - 1998/7/30
N2 - The use of RF (100 to 300 MHz) PEDRI and CW-EPR techniques allows the in vivo study of large animals such as whole rats and rabbits. Recently a PEDRI instrument was modified to also allow CW-EPR spectroscopy with samples of similar size and under the same experimental conditions. In the present study, this CW-EPR and PEDRI apparatus was used to assess the feasibility of the detection of a pyrrolidine nitroxide free radical (2,2,5,5,tetramethylpyrrolidine-1-oxyl-3-carboxylic acid, PCA) in the abdomen of rats. In particular, we have shown that after the PCA administration (4 mmol kg-1 b.w.): (i) the PCA EPR linewidth does not show line broadening due to concentration effects; (ii) a similar PCA up-take phase is observed by EPR and PEDRI; and (iii) the PCA half-lives in the whole abdomen of rats measured with the CW-EPR (T(1/2) = 26 ± 4 min, mean ± sd, n = 10) and PEDRI (T(1/2) = 29 ± 4 min, mean ± sd, n = 4) techniques were not significantly different (p > 0.05). These results show, for the first time, that information about PCA pharmacokinetics obtained by CW-EPR is the same as that from PEDRI under the same experimental conditions.
AB - The use of RF (100 to 300 MHz) PEDRI and CW-EPR techniques allows the in vivo study of large animals such as whole rats and rabbits. Recently a PEDRI instrument was modified to also allow CW-EPR spectroscopy with samples of similar size and under the same experimental conditions. In the present study, this CW-EPR and PEDRI apparatus was used to assess the feasibility of the detection of a pyrrolidine nitroxide free radical (2,2,5,5,tetramethylpyrrolidine-1-oxyl-3-carboxylic acid, PCA) in the abdomen of rats. In particular, we have shown that after the PCA administration (4 mmol kg-1 b.w.): (i) the PCA EPR linewidth does not show line broadening due to concentration effects; (ii) a similar PCA up-take phase is observed by EPR and PEDRI; and (iii) the PCA half-lives in the whole abdomen of rats measured with the CW-EPR (T(1/2) = 26 ± 4 min, mean ± sd, n = 10) and PEDRI (T(1/2) = 29 ± 4 min, mean ± sd, n = 4) techniques were not significantly different (p > 0.05). These results show, for the first time, that information about PCA pharmacokinetics obtained by CW-EPR is the same as that from PEDRI under the same experimental conditions.
UR - http://www.scopus.com/inward/record.url?scp=0031866179&partnerID=8YFLogxK
U2 - 10.1088/0031-9155/43/7/011
DO - 10.1088/0031-9155/43/7/011
M3 - Article
C2 - 9703053
AN - SCOPUS:0031866179
VL - 43
SP - 1899
EP - 1905
JO - Physics in Medicine and Biology
JF - Physics in Medicine and Biology
SN - 0031-9155
IS - 7
ER -