No evidence of apomixis in matroclinal progeny from experimental crosses in the genus Fragaria (strawberry) based on RAPDs

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Abstract

Crossing experiments in Fragaria have frequently resulted in some matroclinal progeny. These have been attributed to either accidental selfing and/or pollen contamination, or apomixis. In this study 202 carefully controlled intra and interspecific crosses in Fragaria were made. No progeny were produced from 164 interploid crosses made between diploids, hexaploids and octoploids but 38 intraploid crosses resulted in 904 F-1 plants, of which 42 (4.6%) were found to be morphologically matroclinal. All matroclinal progeny subsequently studied by RAPDs were found to be hybrids and not apomicts. The absence of apomixis among matroclinal progeny in this study casts doubt on previous reports of apomixis in Fragaria. Our results showed that the complete morphological similarity of progeny to the seed parent, even in recessive characteristics, cannot therefore be taken as evidence of apomixis, and may rather indicate the heterozygosity of the pollen parent, emphasising the need to use DNA markers for confirmation of apomixis.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalEuphytica
Volume171
Issue number2
Early online date4 Aug 2009
DOIs
Publication statusPublished - Jan 2010

Keywords

  • apomictic
  • hybrid origin
  • matroclinal progeny
  • RAPDs
  • strawberry
  • POA-pratensis
  • molecular markers
  • Kentucky Bluegrass
  • flow-cytometry
  • hybrids
  • rosa
  • reproduction
  • genetics
  • caninae
  • parthenogenesis
  • Apomictic
  • Hybrid origin
  • Matroclinal progeny
  • Strawberry

Cite this

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title = "No evidence of apomixis in matroclinal progeny from experimental crosses in the genus Fragaria (strawberry) based on RAPDs",
abstract = "Crossing experiments in Fragaria have frequently resulted in some matroclinal progeny. These have been attributed to either accidental selfing and/or pollen contamination, or apomixis. In this study 202 carefully controlled intra and interspecific crosses in Fragaria were made. No progeny were produced from 164 interploid crosses made between diploids, hexaploids and octoploids but 38 intraploid crosses resulted in 904 F-1 plants, of which 42 (4.6{\%}) were found to be morphologically matroclinal. All matroclinal progeny subsequently studied by RAPDs were found to be hybrids and not apomicts. The absence of apomixis among matroclinal progeny in this study casts doubt on previous reports of apomixis in Fragaria. Our results showed that the complete morphological similarity of progeny to the seed parent, even in recessive characteristics, cannot therefore be taken as evidence of apomixis, and may rather indicate the heterozygosity of the pollen parent, emphasising the need to use DNA markers for confirmation of apomixis.",
keywords = "apomictic, hybrid origin, matroclinal progeny, RAPDs, strawberry, POA-pratensis, molecular markers, Kentucky Bluegrass, flow-cytometry, hybrids, rosa, reproduction, genetics, caninae, parthenogenesis, Apomictic, Hybrid origin, Matroclinal progeny, Strawberry",
author = "Houshang Nosrati and Price, {Adam Huw} and Wilcock, {Christopher Charles}",
year = "2010",
month = "1",
doi = "10.1007/s10681-009-0008-3",
language = "English",
volume = "171",
pages = "193--202",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Netherlands",
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TY - JOUR

T1 - No evidence of apomixis in matroclinal progeny from experimental crosses in the genus Fragaria (strawberry) based on RAPDs

AU - Nosrati, Houshang

AU - Price, Adam Huw

AU - Wilcock, Christopher Charles

PY - 2010/1

Y1 - 2010/1

N2 - Crossing experiments in Fragaria have frequently resulted in some matroclinal progeny. These have been attributed to either accidental selfing and/or pollen contamination, or apomixis. In this study 202 carefully controlled intra and interspecific crosses in Fragaria were made. No progeny were produced from 164 interploid crosses made between diploids, hexaploids and octoploids but 38 intraploid crosses resulted in 904 F-1 plants, of which 42 (4.6%) were found to be morphologically matroclinal. All matroclinal progeny subsequently studied by RAPDs were found to be hybrids and not apomicts. The absence of apomixis among matroclinal progeny in this study casts doubt on previous reports of apomixis in Fragaria. Our results showed that the complete morphological similarity of progeny to the seed parent, even in recessive characteristics, cannot therefore be taken as evidence of apomixis, and may rather indicate the heterozygosity of the pollen parent, emphasising the need to use DNA markers for confirmation of apomixis.

AB - Crossing experiments in Fragaria have frequently resulted in some matroclinal progeny. These have been attributed to either accidental selfing and/or pollen contamination, or apomixis. In this study 202 carefully controlled intra and interspecific crosses in Fragaria were made. No progeny were produced from 164 interploid crosses made between diploids, hexaploids and octoploids but 38 intraploid crosses resulted in 904 F-1 plants, of which 42 (4.6%) were found to be morphologically matroclinal. All matroclinal progeny subsequently studied by RAPDs were found to be hybrids and not apomicts. The absence of apomixis among matroclinal progeny in this study casts doubt on previous reports of apomixis in Fragaria. Our results showed that the complete morphological similarity of progeny to the seed parent, even in recessive characteristics, cannot therefore be taken as evidence of apomixis, and may rather indicate the heterozygosity of the pollen parent, emphasising the need to use DNA markers for confirmation of apomixis.

KW - apomictic

KW - hybrid origin

KW - matroclinal progeny

KW - RAPDs

KW - strawberry

KW - POA-pratensis

KW - molecular markers

KW - Kentucky Bluegrass

KW - flow-cytometry

KW - hybrids

KW - rosa

KW - reproduction

KW - genetics

KW - caninae

KW - parthenogenesis

KW - Apomictic

KW - Hybrid origin

KW - Matroclinal progeny

KW - Strawberry

U2 - 10.1007/s10681-009-0008-3

DO - 10.1007/s10681-009-0008-3

M3 - Article

VL - 171

SP - 193

EP - 202

JO - Euphytica

JF - Euphytica

SN - 0014-2336

IS - 2

ER -