No influence of CO2 on stable isotope analyses of soil waters with OA-ICOS

Matthias Sprenger, Doerthe Tetzlaff, Chris Soulsby

Research output: Contribution to journalArticle

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Abstract

Rationale
It was recently shown that the presence of CO2 affects the stable isotope (δ2H and δ18O values) analysis of water vapor via Wavelength-Scanned Cavity Ring Down Spectroscopy. Here we test how much CO2 is emitted from soil samples and if the CO2 in the headspace influences the isotope analysis with the direct equilibration method by Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS).

Methods
The headspace above different amounts of sparkling water was sampled, and its stable isotopic composition (δ2H and δ18O values) measured by direct equilibration and its CO2 concentration by gas chromatography. In addition, the headspace above soil samples was analyzed in the same way. Furthermore, the gravimetric water content and the loss on ignition were measured for the soil samples.

Results
The experiment with the sparkling water showed that CO2 does not influence the stable isotope analysis by OA-ICOS. CO2 was emitted from the soil samples and correlated with the isotopic fractionation signal, but no causal relationship between the two was determined. Instead, the fractionation signal in pore water isotopes can be explained by soil evaporation and the CO2 can be related to soil moisture and organic matter which both enhance microbial activity.

Conclusions
We found, despite the high CO2 emissions from soil samples, no need for a post-correction of the pore water stable isotope analysis results, since there is no relation between CO2 concentrations and the stable isotope results of vapor samples obtained with OA-ICOS
Original languageEnglish
Pages (from-to)430-436
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume31
Issue number5
Early online date26 Dec 2016
DOIs
Publication statusPublished - 15 Mar 2017

Fingerprint

Isotopes
Spectroscopy
Soils
Water
Fractionation
Value engineering
Soil moisture
Steam
Gas chromatography
Biological materials
Water content
Ignition
Evaporation
Vapors
Wavelength
Chemical analysis
Experiments

Keywords

  • laser spectrometry
  • isotope hydrology
  • C02
  • soil hydrology

Cite this

No influence of CO2 on stable isotope analyses of soil waters with OA-ICOS. / Sprenger, Matthias; Tetzlaff, Doerthe; Soulsby, Chris.

In: Rapid Communications in Mass Spectrometry, Vol. 31, No. 5, 15.03.2017, p. 430-436.

Research output: Contribution to journalArticle

@article{d0d7ffdd983549f88ac7ceafbbf97b1a,
title = "No influence of CO2 on stable isotope analyses of soil waters with OA-ICOS",
abstract = "RationaleIt was recently shown that the presence of CO2 affects the stable isotope (δ2H and δ18O values) analysis of water vapor via Wavelength-Scanned Cavity Ring Down Spectroscopy. Here we test how much CO2 is emitted from soil samples and if the CO2 in the headspace influences the isotope analysis with the direct equilibration method by Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS).MethodsThe headspace above different amounts of sparkling water was sampled, and its stable isotopic composition (δ2H and δ18O values) measured by direct equilibration and its CO2 concentration by gas chromatography. In addition, the headspace above soil samples was analyzed in the same way. Furthermore, the gravimetric water content and the loss on ignition were measured for the soil samples.ResultsThe experiment with the sparkling water showed that CO2 does not influence the stable isotope analysis by OA-ICOS. CO2 was emitted from the soil samples and correlated with the isotopic fractionation signal, but no causal relationship between the two was determined. Instead, the fractionation signal in pore water isotopes can be explained by soil evaporation and the CO2 can be related to soil moisture and organic matter which both enhance microbial activity.ConclusionsWe found, despite the high CO2 emissions from soil samples, no need for a post-correction of the pore water stable isotope analysis results, since there is no relation between CO2 concentrations and the stable isotope results of vapor samples obtained with OA-ICOS",
keywords = "laser spectrometry, isotope hydrology, C02, soil hydrology",
author = "Matthias Sprenger and Doerthe Tetzlaff and Chris Soulsby",
note = "Acknowledgements We are thankful for the support by Audrey Innes with the stable isotope, LOI, and GWC analysis. We thank Jonathan Dick for suggesting that we use sparkling water to generate different CO2 concentrations in the headspace and Claire Tunaley for proof reading. We further highly appreciate the help of David Galloway and Michael Mcgibbon from the School of Biological Sciences, University of Aberdeen, with the CO2 analysis. We are also thankful for the support by Robert Provencal and Doug S. Baer regarding the technical aspects of the isotope analyzer. We would also like to thank the European Research Council (ERC, Project No. GA 335910 VeWa) and the Natural Environment Research Council (NERC, Project No. NE/K000268/1) for funding. We thank three anonymous reviewers for their valuable feedback that helped to improve the manuscript.",
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T1 - No influence of CO2 on stable isotope analyses of soil waters with OA-ICOS

AU - Sprenger, Matthias

AU - Tetzlaff, Doerthe

AU - Soulsby, Chris

N1 - Acknowledgements We are thankful for the support by Audrey Innes with the stable isotope, LOI, and GWC analysis. We thank Jonathan Dick for suggesting that we use sparkling water to generate different CO2 concentrations in the headspace and Claire Tunaley for proof reading. We further highly appreciate the help of David Galloway and Michael Mcgibbon from the School of Biological Sciences, University of Aberdeen, with the CO2 analysis. We are also thankful for the support by Robert Provencal and Doug S. Baer regarding the technical aspects of the isotope analyzer. We would also like to thank the European Research Council (ERC, Project No. GA 335910 VeWa) and the Natural Environment Research Council (NERC, Project No. NE/K000268/1) for funding. We thank three anonymous reviewers for their valuable feedback that helped to improve the manuscript.

PY - 2017/3/15

Y1 - 2017/3/15

N2 - RationaleIt was recently shown that the presence of CO2 affects the stable isotope (δ2H and δ18O values) analysis of water vapor via Wavelength-Scanned Cavity Ring Down Spectroscopy. Here we test how much CO2 is emitted from soil samples and if the CO2 in the headspace influences the isotope analysis with the direct equilibration method by Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS).MethodsThe headspace above different amounts of sparkling water was sampled, and its stable isotopic composition (δ2H and δ18O values) measured by direct equilibration and its CO2 concentration by gas chromatography. In addition, the headspace above soil samples was analyzed in the same way. Furthermore, the gravimetric water content and the loss on ignition were measured for the soil samples.ResultsThe experiment with the sparkling water showed that CO2 does not influence the stable isotope analysis by OA-ICOS. CO2 was emitted from the soil samples and correlated with the isotopic fractionation signal, but no causal relationship between the two was determined. Instead, the fractionation signal in pore water isotopes can be explained by soil evaporation and the CO2 can be related to soil moisture and organic matter which both enhance microbial activity.ConclusionsWe found, despite the high CO2 emissions from soil samples, no need for a post-correction of the pore water stable isotope analysis results, since there is no relation between CO2 concentrations and the stable isotope results of vapor samples obtained with OA-ICOS

AB - RationaleIt was recently shown that the presence of CO2 affects the stable isotope (δ2H and δ18O values) analysis of water vapor via Wavelength-Scanned Cavity Ring Down Spectroscopy. Here we test how much CO2 is emitted from soil samples and if the CO2 in the headspace influences the isotope analysis with the direct equilibration method by Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS).MethodsThe headspace above different amounts of sparkling water was sampled, and its stable isotopic composition (δ2H and δ18O values) measured by direct equilibration and its CO2 concentration by gas chromatography. In addition, the headspace above soil samples was analyzed in the same way. Furthermore, the gravimetric water content and the loss on ignition were measured for the soil samples.ResultsThe experiment with the sparkling water showed that CO2 does not influence the stable isotope analysis by OA-ICOS. CO2 was emitted from the soil samples and correlated with the isotopic fractionation signal, but no causal relationship between the two was determined. Instead, the fractionation signal in pore water isotopes can be explained by soil evaporation and the CO2 can be related to soil moisture and organic matter which both enhance microbial activity.ConclusionsWe found, despite the high CO2 emissions from soil samples, no need for a post-correction of the pore water stable isotope analysis results, since there is no relation between CO2 concentrations and the stable isotope results of vapor samples obtained with OA-ICOS

KW - laser spectrometry

KW - isotope hydrology

KW - C02

KW - soil hydrology

U2 - 10.1002/rcm.7815

DO - 10.1002/rcm.7815

M3 - Article

VL - 31

SP - 430

EP - 436

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 5

ER -