NOS1 polymorphism is associated with atopy but not exhaled nitric oxide levels in healthy children

M. Ali, S. Khoo, Stephen William Turner, S. M. Stick, P. Le Souef, P. Franklin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Exhaled nitric oxide (FENO ) is raised in atopy. The mechanism for this is unclear. The aim of this study was to investigate whether the number of AAT repeats in intron 20 of the NOS1 gene, recently associated with variations in FENO in adults with asthma and cystic fibrosis, was associated with the raised FENO in healthy atopic children. Eighty-seven healthy children (44 girls, 42 atopic, age range 6-18 years) underwent measurements of FENO , spirometry, airway responsiveness and skin prick testing. Genotyping was carried out to determine the number of AAT repeats. There was no association between the number of AAT repeats and FENO in either the whole sample of healthy children (n = 87) or in the subsample of healthy atopics (n = 42). However, a greater number of atopic children had two high repeat alleles compared with non-atopic children (33.3% vs. 13.6%, respectively, p = 0.03). This suggests that variations in the NOS1 gene may contribute to atopy without this relationship being reflected by FENO .

Original languageEnglish
Pages (from-to)261-265
Number of pages4
JournalPediatric Allergy & Immunology
Volume14
Issue number4
DOIs
Publication statusPublished - Aug 2003

Keywords

  • exhaled nitric oxide
  • nitric oxide synthase
  • atopy
  • children
  • ASTHMA
  • SYNTHASE
  • GENE
  • RESPONSIVENESS
  • DISEASE

Cite this

NOS1 polymorphism is associated with atopy but not exhaled nitric oxide levels in healthy children. / Ali, M.; Khoo, S.; Turner, Stephen William; Stick, S. M.; Le Souef, P.; Franklin, P.

In: Pediatric Allergy & Immunology, Vol. 14, No. 4, 08.2003, p. 261-265.

Research output: Contribution to journalArticle

Ali, M. ; Khoo, S. ; Turner, Stephen William ; Stick, S. M. ; Le Souef, P. ; Franklin, P. / NOS1 polymorphism is associated with atopy but not exhaled nitric oxide levels in healthy children. In: Pediatric Allergy & Immunology. 2003 ; Vol. 14, No. 4. pp. 261-265.
@article{b58db328c41741089b8d5804058427ae,
title = "NOS1 polymorphism is associated with atopy but not exhaled nitric oxide levels in healthy children",
abstract = "Exhaled nitric oxide (FENO ) is raised in atopy. The mechanism for this is unclear. The aim of this study was to investigate whether the number of AAT repeats in intron 20 of the NOS1 gene, recently associated with variations in FENO in adults with asthma and cystic fibrosis, was associated with the raised FENO in healthy atopic children. Eighty-seven healthy children (44 girls, 42 atopic, age range 6-18 years) underwent measurements of FENO , spirometry, airway responsiveness and skin prick testing. Genotyping was carried out to determine the number of AAT repeats. There was no association between the number of AAT repeats and FENO in either the whole sample of healthy children (n = 87) or in the subsample of healthy atopics (n = 42). However, a greater number of atopic children had two high repeat alleles compared with non-atopic children (33.3{\%} vs. 13.6{\%}, respectively, p = 0.03). This suggests that variations in the NOS1 gene may contribute to atopy without this relationship being reflected by FENO .",
keywords = "exhaled nitric oxide, nitric oxide synthase, atopy, children, ASTHMA, SYNTHASE, GENE, RESPONSIVENESS, DISEASE",
author = "M. Ali and S. Khoo and Turner, {Stephen William} and Stick, {S. M.} and {Le Souef}, P. and P. Franklin",
year = "2003",
month = "8",
doi = "10.1034/j.1399-3038.2003.00065.x",
language = "English",
volume = "14",
pages = "261--265",
journal = "Pediatric Allergy & Immunology",
issn = "0905-6157",
publisher = "Blackwell Munksgaard",
number = "4",

}

TY - JOUR

T1 - NOS1 polymorphism is associated with atopy but not exhaled nitric oxide levels in healthy children

AU - Ali, M.

AU - Khoo, S.

AU - Turner, Stephen William

AU - Stick, S. M.

AU - Le Souef, P.

AU - Franklin, P.

PY - 2003/8

Y1 - 2003/8

N2 - Exhaled nitric oxide (FENO ) is raised in atopy. The mechanism for this is unclear. The aim of this study was to investigate whether the number of AAT repeats in intron 20 of the NOS1 gene, recently associated with variations in FENO in adults with asthma and cystic fibrosis, was associated with the raised FENO in healthy atopic children. Eighty-seven healthy children (44 girls, 42 atopic, age range 6-18 years) underwent measurements of FENO , spirometry, airway responsiveness and skin prick testing. Genotyping was carried out to determine the number of AAT repeats. There was no association between the number of AAT repeats and FENO in either the whole sample of healthy children (n = 87) or in the subsample of healthy atopics (n = 42). However, a greater number of atopic children had two high repeat alleles compared with non-atopic children (33.3% vs. 13.6%, respectively, p = 0.03). This suggests that variations in the NOS1 gene may contribute to atopy without this relationship being reflected by FENO .

AB - Exhaled nitric oxide (FENO ) is raised in atopy. The mechanism for this is unclear. The aim of this study was to investigate whether the number of AAT repeats in intron 20 of the NOS1 gene, recently associated with variations in FENO in adults with asthma and cystic fibrosis, was associated with the raised FENO in healthy atopic children. Eighty-seven healthy children (44 girls, 42 atopic, age range 6-18 years) underwent measurements of FENO , spirometry, airway responsiveness and skin prick testing. Genotyping was carried out to determine the number of AAT repeats. There was no association between the number of AAT repeats and FENO in either the whole sample of healthy children (n = 87) or in the subsample of healthy atopics (n = 42). However, a greater number of atopic children had two high repeat alleles compared with non-atopic children (33.3% vs. 13.6%, respectively, p = 0.03). This suggests that variations in the NOS1 gene may contribute to atopy without this relationship being reflected by FENO .

KW - exhaled nitric oxide

KW - nitric oxide synthase

KW - atopy

KW - children

KW - ASTHMA

KW - SYNTHASE

KW - GENE

KW - RESPONSIVENESS

KW - DISEASE

U2 - 10.1034/j.1399-3038.2003.00065.x

DO - 10.1034/j.1399-3038.2003.00065.x

M3 - Article

VL - 14

SP - 261

EP - 265

JO - Pediatric Allergy & Immunology

JF - Pediatric Allergy & Immunology

SN - 0905-6157

IS - 4

ER -