The concentration of lysinoalanine in a sample of barley treated with 35 g NaOH kg-1 was found to be 1.16 g kg-1 compared with zero concentration in control samples. Although such a level in the diet might result in a reduced growth rate and produce kidney lesions in the rat, the nutritional risk to farm stock, including non-ruminant animals, would appear to be small if allowance is made for reduced concentrations of important amino acids when formulating diets.
DAVIDSON, J., MCINTOSH, A. D., & Milne, E. (1982). Note on the lysinoalanine content of alkali-treated barley. Animal Feed Science and Technology, 7(2), 217-220. https://doi.org/10.1016/0377-8401(82)90055-4