Novel cyanobacterial biosensor for detection of herbicides

Cuiying Shao, C. J. Howe, Andrew Justin Radcliffe Porter, Lesley Anne Glover

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The aim of this work was to generate a cyanobacterial biosensor that could be used to detect herbicides and other environmental pollutants. A representative freshwater cyanobacterium, Synechocystis sp. strain PCC6803, was chromosomally marked with the luciferase gene luc (from the firefly Photinus pyralis) to create a novel bioluminescent cyanobacterial strain. Successful expression of the luc gene during growth of Synechocystis sp. strain PCC6803 cultures was characterized by measuring optical density and bioluminescence. Bioluminescence was optimized with regard to uptake of the luciferase substrate, luciferin, and the physiology of the cyanobacterium. Bioassays demonstrated that a novel luminescent cyanobacterial biosensor has been developed which responded to a range of compounds including different herbicide types and other toxins. This biosensor is expected to provide new opportunities for the rapid screening of environmental samples or for the investigation of potential environmental damage.

Original languageEnglish
Pages (from-to)5026-5033
Number of pages7
JournalApplied and Environmental Microbiology
Volume68
Issue number10
DOIs
Publication statusPublished - 2002

Keywords

  • PSEUDOMONAS-FLUORESCENS
  • BACTERIAL BIOSENSORS
  • IMMOBILIZED ALGAE
  • CHLOROPHYLL
  • TOXICITY
  • ASSAY
  • BIOLUMINESCENCE
  • CONSTRUCTION
  • MEMBRANES
  • YIELD

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