Novel electrochemical nanoswitch biosensor based on self-assembled pH-sensitive continuous circular DNA

Xian Chen* (Corresponding Author), Le Yao, Yu Chao Wang, Qin Chen, Hai Deng, Zhen Yu Lin, Huang Hao Yang (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nucleic acid nanoswitches have a status that cannot be ignored in the field of biosensing due to the excellent biocompatibility and flexibility of design. In our current research, we have constructed a new electrochemical platform based on self-assembled pH-sensitive continuous circular DNA nanoswitch for miRNA-21 detection. We elaborately designed an inside ring probe (IRP) which could form a circle when complemented with an outside ring probe (ORP). Under the weakly acidic condition, IRPs and ORPs are self-assembled into continuous annular DNA, meanwhile, the nanoswitch is activated. However, if it is not a weakly acidic environment with a pH equal to 6, these circles are separated and the nanoswitch cannot be triggered. Therefore, the biosensor doesn't work. Only when the pH is 6, can the nanoswitch be activated. Consequently, a large number of RuHex will accumulate on the continuous annular DNA, which leads to highly sensitive detection of miRNA-21, with concentration ranged from 10 –15 to 10 -8 M and limit of detection down to 0.84 fM. More importantly, this nanoswitch-based biosensor can directly detect the target microRNA in human serum without pretreatment. Therefore, the proposed novel electrochemical DNA nanoswitch will have broad application prospects in biomarker detection and clinical diagnosis.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalBiosensors and Bioelectronics
Volume131
Early online date20 Feb 2019
DOIs
Publication statusPublished - 15 Apr 2019

Fingerprint

Circular DNA
Biosensing Techniques
MicroRNAs
Biosensors
DNA
Biomarkers
Biocompatibility
Nucleic Acids
Limit of Detection
Nucleic acids
Serum
Research

Keywords

  • Biosensor
  • Continuous circular DNA
  • Electrochemical biosensor
  • MiRNA detection
  • Nanoswitch biosensor
  • PH-sensitive

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Novel electrochemical nanoswitch biosensor based on self-assembled pH-sensitive continuous circular DNA. / Chen, Xian (Corresponding Author); Yao, Le; Wang, Yu Chao; Chen, Qin; Deng, Hai; Lin, Zhen Yu; Yang, Huang Hao (Corresponding Author).

In: Biosensors and Bioelectronics, Vol. 131, 15.04.2019, p. 274-279.

Research output: Contribution to journalArticle

Chen, Xian ; Yao, Le ; Wang, Yu Chao ; Chen, Qin ; Deng, Hai ; Lin, Zhen Yu ; Yang, Huang Hao. / Novel electrochemical nanoswitch biosensor based on self-assembled pH-sensitive continuous circular DNA. In: Biosensors and Bioelectronics. 2019 ; Vol. 131. pp. 274-279.
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abstract = "Nucleic acid nanoswitches have a status that cannot be ignored in the field of biosensing due to the excellent biocompatibility and flexibility of design. In our current research, we have constructed a new electrochemical platform based on self-assembled pH-sensitive continuous circular DNA nanoswitch for miRNA-21 detection. We elaborately designed an inside ring probe (IRP) which could form a circle when complemented with an outside ring probe (ORP). Under the weakly acidic condition, IRPs and ORPs are self-assembled into continuous annular DNA, meanwhile, the nanoswitch is activated. However, if it is not a weakly acidic environment with a pH equal to 6, these circles are separated and the nanoswitch cannot be triggered. Therefore, the biosensor doesn't work. Only when the pH is 6, can the nanoswitch be activated. Consequently, a large number of RuHex will accumulate on the continuous annular DNA, which leads to highly sensitive detection of miRNA-21, with concentration ranged from 10 –15 to 10 -8 M and limit of detection down to 0.84 fM. More importantly, this nanoswitch-based biosensor can directly detect the target microRNA in human serum without pretreatment. Therefore, the proposed novel electrochemical DNA nanoswitch will have broad application prospects in biomarker detection and clinical diagnosis.",
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