Abstract
Nucleic acid nanoswitches have a status that cannot be ignored in the field of biosensing due to the excellent biocompatibility and flexibility of design. In our current research, we have constructed a new electrochemical platform based on self-assembled pH-sensitive continuous circular DNA nanoswitch for miRNA-21 detection. We elaborately designed an inside ring probe (IRP) which could form a circle when complemented with an outside ring probe (ORP). Under the weakly acidic condition, IRPs and ORPs are self-assembled into continuous annular DNA, meanwhile, the nanoswitch is activated. However, if it is not a weakly acidic environment with a pH equal to 6, these circles are separated and the nanoswitch cannot be triggered. Therefore, the biosensor doesn't work. Only when the pH is 6, can the nanoswitch be activated. Consequently, a large number of RuHex will accumulate on the continuous annular DNA, which leads to highly sensitive detection of miRNA-21, with concentration ranged from 10 –15 to 10 -8 M and limit of detection down to 0.84 fM. More importantly, this nanoswitch-based biosensor can directly detect the target microRNA in human serum without pretreatment. Therefore, the proposed novel electrochemical DNA nanoswitch will have broad application prospects in biomarker detection and clinical diagnosis.
| Original language | English |
|---|---|
| Pages (from-to) | 274-279 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 131 |
| Early online date | 20 Feb 2019 |
| DOIs | |
| Publication status | Published - 15 Apr 2019 |
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Keywords
- Biosensor
- Continuous circular DNA
- Electrochemical biosensor
- MiRNA detection
- Nanoswitch biosensor
- PH-sensitive
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
Novel electrochemical nanoswitch biosensor based on self-assembled pH-sensitive continuous circular DNA. / Chen, Xian (Corresponding Author); Yao, Le; Wang, Yu Chao; Chen, Qin; Deng, Hai; Lin, Zhen Yu; Yang, Huang Hao (Corresponding Author).
In: Biosensors and Bioelectronics, Vol. 131, 15.04.2019, p. 274-279.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel electrochemical nanoswitch biosensor based on self-assembled pH-sensitive continuous circular DNA
AU - Chen, Xian
AU - Yao, Le
AU - Wang, Yu Chao
AU - Chen, Qin
AU - Deng, Hai
AU - Lin, Zhen Yu
AU - Yang, Huang Hao
N1 - This work was financially supported by China Scholarship Council, China (grant no. 201706655025), Department of Education, Fujian Province, China (grant no. JK2017006) and National Natural Science Foundation of China, China (grant no. 21575027).
PY - 2019/4/15
Y1 - 2019/4/15
N2 - Nucleic acid nanoswitches have a status that cannot be ignored in the field of biosensing due to the excellent biocompatibility and flexibility of design. In our current research, we have constructed a new electrochemical platform based on self-assembled pH-sensitive continuous circular DNA nanoswitch for miRNA-21 detection. We elaborately designed an inside ring probe (IRP) which could form a circle when complemented with an outside ring probe (ORP). Under the weakly acidic condition, IRPs and ORPs are self-assembled into continuous annular DNA, meanwhile, the nanoswitch is activated. However, if it is not a weakly acidic environment with a pH equal to 6, these circles are separated and the nanoswitch cannot be triggered. Therefore, the biosensor doesn't work. Only when the pH is 6, can the nanoswitch be activated. Consequently, a large number of RuHex will accumulate on the continuous annular DNA, which leads to highly sensitive detection of miRNA-21, with concentration ranged from 10 –15 to 10 -8 M and limit of detection down to 0.84 fM. More importantly, this nanoswitch-based biosensor can directly detect the target microRNA in human serum without pretreatment. Therefore, the proposed novel electrochemical DNA nanoswitch will have broad application prospects in biomarker detection and clinical diagnosis.
AB - Nucleic acid nanoswitches have a status that cannot be ignored in the field of biosensing due to the excellent biocompatibility and flexibility of design. In our current research, we have constructed a new electrochemical platform based on self-assembled pH-sensitive continuous circular DNA nanoswitch for miRNA-21 detection. We elaborately designed an inside ring probe (IRP) which could form a circle when complemented with an outside ring probe (ORP). Under the weakly acidic condition, IRPs and ORPs are self-assembled into continuous annular DNA, meanwhile, the nanoswitch is activated. However, if it is not a weakly acidic environment with a pH equal to 6, these circles are separated and the nanoswitch cannot be triggered. Therefore, the biosensor doesn't work. Only when the pH is 6, can the nanoswitch be activated. Consequently, a large number of RuHex will accumulate on the continuous annular DNA, which leads to highly sensitive detection of miRNA-21, with concentration ranged from 10 –15 to 10 -8 M and limit of detection down to 0.84 fM. More importantly, this nanoswitch-based biosensor can directly detect the target microRNA in human serum without pretreatment. Therefore, the proposed novel electrochemical DNA nanoswitch will have broad application prospects in biomarker detection and clinical diagnosis.
KW - Biosensor
KW - Continuous circular DNA
KW - Electrochemical biosensor
KW - MiRNA detection
KW - Nanoswitch biosensor
KW - PH-sensitive
UR - http://www.scopus.com/inward/record.url?scp=85062276425&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2019.02.004
DO - 10.1016/j.bios.2019.02.004
M3 - Article
C2 - 30849727
AN - SCOPUS:85062276425
VL - 131
SP - 274
EP - 279
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
ER -