Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks

David Johnson, J. R. Leake, D. J. Read

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested.

The cores have windows covered with nylon mesh of 35 mum pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a 'bait' seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of P-33 from soil to plants outside the cores (experiment 2) were examined.

Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot P-33 concentrations of plants with mycelial access to (PO4)-P-33-labelled cores were 10-fold greater than those which had no mycelial access.

It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature. (C) New Phytologist (2001).

Original languageEnglish
Pages (from-to)555-562
Number of pages7
JournalNew Phytologist
Volume152
Issue number3
DOIs
Publication statusPublished - Dec 2001

Keywords

  • AM mycorrhizal colonization
  • Trifolium repens (white clover)
  • mycelial networks
  • phosphorus uptake
  • P-33
  • hyphal exclusion
  • VESICULAR-ARBUSCULAR MYCORRHIZA
  • TRIFOLIUM-SUBTERRANEUM L
  • CHOUARD EX ROTHM
  • HYPHAL TRANSPORT
  • EXTERNAL HYPHAE
  • PHOSPHORUS
  • FUNGI
  • ROOT
  • INFECTION
  • PLANT

Cite this

Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. / Johnson, David; Leake, J. R.; Read, D. J.

In: New Phytologist, Vol. 152, No. 3, 12.2001, p. 555-562.

Research output: Contribution to journalArticle

Johnson, David ; Leake, J. R. ; Read, D. J. / Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. In: New Phytologist. 2001 ; Vol. 152, No. 3. pp. 555-562.
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