Abstract
Near-infrared fluorescence imaging in the 1500–1700 nm sub-window (NIR-IIb) has shown a deeper penetration depth, higher resolution and zero auto-fluorescence for biomedical imaging. Till now, very 2 few small molecule NIR-IIb fluorophores have been reported due to the extremely rare organic NIR-IIb
skeleton and a notorious aggregation-caused quenching (ACQ) effect in aqueous solution. In this study, highly twisted NIR-II small molecule fluorophores such as HL3 (45.5 at the S0 state) with the emission wavelength extending into the NIR-IIb region were designed and synthesized using an aggregationinduced
emission (AIE) strategy. HL3 dots showed a remarkable increase in fluorescence intensity with a QY of 11.7% in the NIR-II window (>1000 nm) and 0.05% in the NIR-IIb region (>1550 nm) in water. High-resolution in vivo imaging of the whole body, cerebral vasculature, and lymphatic drainage beyond
1550 nm was achieved using NIR-II AIE HL3 dots for the first time. These attractive results may promote the development of small-molecule NIR-IIb fluorophores with the maximum emission wavelength beyond 1500 nm with a deeper penetration depth and higher resolution.
Original language | English |
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Pages (from-to) | 2621-2626 |
Number of pages | 6 |
Journal | Chemical Science |
Volume | 11 |
Issue number | 10 |
Early online date | 6 Feb 2020 |
DOIs | |
Publication status | Published - 14 Mar 2020 |
Bibliographical note
This work was partially supported by grants from NSFC (81773674, 81573383, and 21473041), NSFHP (2017CFA024, 2017CFB711, and 2016ACA126), the Applied Basic Research Program of WMBST (2019020701011429), Tibet Autonomous Region Science and Technology Plan Project Key Project (XZ201901-GB-11), Project First-Class Disciplines Development Supported by Chengdu University of Traditional Chinese Medicine (CZYJC1903), and Health Commission of Hubei Province Scientific Research Project (WJ2019M177 and WJ2019M178).Keywords
- biomedical imaging
- AG2S QUANTUM DOTS
- DESIGN
- NANOPROBES
- DYES
- FLUORESCENCE
- BODY
- BRAIN
- WINDOW
- BRIGHT