Novel tetracycline resistance gene, tet(32), in the Clostridium-related human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens

C M Melville, K P Scott, D K Mercer, H J Flint

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221(R). The predicted gene product of tet(32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.

Original languageEnglish
Pages (from-to)3246-3249
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume45
Issue number11
Publication statusPublished - Nov 2001

Keywords

  • HUMAN GUT
  • BACTERIA
  • DETERMINANTS
  • EXPRESSION
  • TET(W)
  • DNA

Cite this

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title = "Novel tetracycline resistance gene, tet(32), in the Clostridium-related human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens",
abstract = "A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221(R). The predicted gene product of tet(32) has 76{\%} amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.",
keywords = "HUMAN GUT, BACTERIA, DETERMINANTS, EXPRESSION, TET(W), DNA",
author = "Melville, {C M} and Scott, {K P} and Mercer, {D K} and Flint, {H J}",
year = "2001",
month = "11",
language = "English",
volume = "45",
pages = "3246--3249",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "AMER SOC MICROBIOLOGY",
number = "11",

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TY - JOUR

T1 - Novel tetracycline resistance gene, tet(32), in the Clostridium-related human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens

AU - Melville, C M

AU - Scott, K P

AU - Mercer, D K

AU - Flint, H J

PY - 2001/11

Y1 - 2001/11

N2 - A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221(R). The predicted gene product of tet(32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.

AB - A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221(R). The predicted gene product of tet(32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.

KW - HUMAN GUT

KW - BACTERIA

KW - DETERMINANTS

KW - EXPRESSION

KW - TET(W)

KW - DNA

M3 - Article

VL - 45

SP - 3246

EP - 3249

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 11

ER -