Oomycete plant pathogens use electric fields to target roots

Pieter Van West, B. M. Morris, Brian Reid, Alex Asante Appiah, M. C. Osborne, T. A. Campbell, S. J. Shepherd, Neil Andrew Robert Gow

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Plant roots generate electrical currents and associated electrical fields as a consequence of electrogenic ion transport at the root surface. Here we demonstrate that the attraction of swimming zoospores of oomycete plant pathogens to plant roots is mediated in part by electrotaxis in natural root-generated electric fields. The zones of accumulation of anode- or cathode-seeking zoospores adjacent to intact and wounded root surfaces correlated with their in vitro electrotactic behavior. Manipulation of the root electrical field was reflected in changes in the pattern of zoospore accumulation and imposed focal electrical fields were capable of overriding endogenous signals at the root surface. The overall pattern of zoospore accumulation around roots was not affected by the presence of amino acids at concentrations expected within the rhizosphere, although higher concentrations induced encystment and reduced root targeting. The data suggest that electrical signals can augment or override chemical ones in mediating short-range tactic responses of oomycete zoospores at root surfaces.

Original languageEnglish
Pages (from-to)790-798
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume15
Issue number8
DOIs
Publication statusPublished - Aug 2002

Keywords

  • chemotaxis
  • Phytophthora
  • Pythium
  • PHYTOPHTHORA-PALMIVORA
  • IONIC CURRENTS
  • PYTHIUM-APHANIDERMATUM
  • PHYTOPATHOGENIC FUNGI
  • VIBRATING PROBE
  • ZOOSPORES
  • INFECTION
  • RESPONSES
  • AGROBACTERIUM
  • ELECTROTAXIS

Cite this

Oomycete plant pathogens use electric fields to target roots. / Van West, Pieter; Morris, B. M.; Reid, Brian; Appiah, Alex Asante; Osborne, M. C.; Campbell, T. A.; Shepherd, S. J.; Gow, Neil Andrew Robert.

In: Molecular Plant-Microbe Interactions, Vol. 15, No. 8, 08.2002, p. 790-798.

Research output: Contribution to journalArticle

Van West, P, Morris, BM, Reid, B, Appiah, AA, Osborne, MC, Campbell, TA, Shepherd, SJ & Gow, NAR 2002, 'Oomycete plant pathogens use electric fields to target roots', Molecular Plant-Microbe Interactions, vol. 15, no. 8, pp. 790-798. https://doi.org/10.1094/MPMI.2002.15.8.790
Van West, Pieter ; Morris, B. M. ; Reid, Brian ; Appiah, Alex Asante ; Osborne, M. C. ; Campbell, T. A. ; Shepherd, S. J. ; Gow, Neil Andrew Robert. / Oomycete plant pathogens use electric fields to target roots. In: Molecular Plant-Microbe Interactions. 2002 ; Vol. 15, No. 8. pp. 790-798.
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AB - Plant roots generate electrical currents and associated electrical fields as a consequence of electrogenic ion transport at the root surface. Here we demonstrate that the attraction of swimming zoospores of oomycete plant pathogens to plant roots is mediated in part by electrotaxis in natural root-generated electric fields. The zones of accumulation of anode- or cathode-seeking zoospores adjacent to intact and wounded root surfaces correlated with their in vitro electrotactic behavior. Manipulation of the root electrical field was reflected in changes in the pattern of zoospore accumulation and imposed focal electrical fields were capable of overriding endogenous signals at the root surface. The overall pattern of zoospore accumulation around roots was not affected by the presence of amino acids at concentrations expected within the rhizosphere, although higher concentrations induced encystment and reduced root targeting. The data suggest that electrical signals can augment or override chemical ones in mediating short-range tactic responses of oomycete zoospores at root surfaces.

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