Optoelectronic tweezers for the measurement of the relative stiffness of erythrocytes

Steven L. Neale, Nimesh Mody, Colin Selman, Jonathan M. Cooper

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

In this paper we describe the first use of Optoelectronic Tweezers (OET), an optically controlled micromanipulation method, to measure the relative stiffness of erythrocytes in mice. Cell stiffness is an important measure of cell health and in the case of erythrocytes, the most elastic cells in the body, an increase in cell stiffness can indicate pathologies such as type II diabetes mellitus or hypertension (high blood pressure). OET uses a photoconductive device to convert an optical pattern into and electrical pattern. The electrical fields will create a dipole within any polarisable particles in the device, such as cells, and non-uniformities of the field can be used to place unequal forces onto each side of the dipole thus moving the particle. In areas of the device where there are no field gradients, areas of constant illumination, the force on each side of the dipole will be equal, keeping the cell stationary, but as there are opposing forces on each side of the cell it will be stretched. The force each cell will experience will differ slightly so the stretching will depend on the cells polarisability as well as its stiffness. Because of this a relative stiffness rather than absolute stiffness is measured. We show that with standard conditions (20Vpp, 1.5MHz, 10mSm-1 medium conductivity) the cell’s diameter changes by around 10% for healthy mouse erythrocytes and we show that due to the low light intensities required for OET, relative to conventional optical tweezers, multiple cells can be measured simultaneously.
Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation IX
EditorsKishan Dholakia, Gabriel C Spalding
PublisherSPIE
Volume8458
ISBN (Print)9780819491756
DOIs
Publication statusPublished - 9 Oct 2012
EventSPIE NanoScience + Engineering - San Diego, United States
Duration: 12 Aug 201216 Aug 2012

Conference

ConferenceSPIE NanoScience + Engineering
CountryUnited States
CitySan Diego
Period12/08/1216/08/12

Fingerprint Dive into the research topics of 'Optoelectronic tweezers for the measurement of the relative stiffness of erythrocytes'. Together they form a unique fingerprint.

  • Cite this

    Neale, S. L., Mody, N., Selman, C., & Cooper, J. M. (2012). Optoelectronic tweezers for the measurement of the relative stiffness of erythrocytes. In K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation IX (Vol. 8458). SPIE. https://doi.org/10.1117/12.928839