Oral bacterial diversity is inversely correlated with mucosal inflammation

Karolin Hijazi*, Roderick W. Morrison, Indrani Mukhopadhya, Brennan Martin, Matthew Gemmell, Sophie Shaw, Francesco Santoro

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Objective
We investigated the relationship amongst the oral mucosal bacterial community, clinical severity and inflammatory markers in the two most common immune‐mediated oral mucosal diseases, namely recurrent aphthous stomatitis (RAS) and oral lichen planus (LP).

Methods
Patients with RAS (n=15), LP (n=18) and healthy controls (n=13) were recruited using criteria to reduce the effect of factors that influence the microbiota structure independently of oral mucosal disease. Clinical severity was quantified using validated scoring methods. DNA was extracted from oral mucosal swabs for 16S rRNA gene high‐throughput sequencing. Salivary cytokines were measured using Cytometric Bead Assays. Correlation studies were conducted amongst microbial diversity, clinical scores and cytokine concentrations.

Results
We observed a significant reduction of bacterial diversity in LP and RAS patients compared to controls (P=0.021 and 0.044, respectively). Reduced bacterial diversity in LP and RAS correlated with increased clinical scores of the two conditions (⍴= ‐0.551 to ‐0.714). A negative correlation was observed between microbial diversity and salivary interferon‐γ, interleukin‐17A, interleukin‐1β (⍴= ‐0.325 to ‐0.449).

Conclusions
This study reports reduced oral microbial diversity in the context of increased mucosal inflammation and supports the role for microbial diversity as a marker or contributor to oral mucosal inflammatory disease activity and development.
Original languageEnglish
Number of pages10
JournalOral Diseases
Early online date3 Jun 2020
DOIs
Publication statusE-pub ahead of print - 3 Jun 2020

Keywords

  • oral ulcer
  • oral mucosa
  • lichen planus
  • microbiota
  • cytokines
  • saliva
  • BIOFILMS
  • DISEASE
  • MICROBIAL COMMUNITY
  • T-CELLS
  • SALIVA

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Oral bacterial diversity is inversely correlated with mucosal inflammation'. Together they form a unique fingerprint.

  • Profiles

    Cite this