Abstract
OBJECTIVES: This study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration.
DESIGN: This was an observational study to determine oral HR-HPV prevalence in the study population. A case-control study was performed to explore the association between infection and folate status.
SETTING: This study was conducted in Sheffield, UK, between April 2013 and August 2014.
PARTICIPANTS: Seven hundred participants, aged 18-60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223).
INTERVENTIONS: Participants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration.
OUTCOME MEASURES: The prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection.
RESULTS: The prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection.
CONCLUSIONS: The prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity.
TRIAL REGISTRATION NUMBER: ID14106.
| Original language | English |
|---|---|
| Article number | e022497 |
| Journal | BMJ Open |
| Volume | 8 |
| Issue number | 8 |
| Early online date | 19 Aug 2018 |
| DOIs | |
| Publication status | Published - Nov 2018 |
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Oral human papillomavirus infection in England and associated risk factors : a case-control study. / Hearnden, Vanessa (Corresponding Author); Murdoch, Craig; D'Apice, Katy; Duthie, Susan; Hayward, Nicholas J; Powers, Hilary Jane.
In: BMJ Open, Vol. 8, No. 8, e022497, 11.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oral human papillomavirus infection in England and associated risk factors
T2 - a case-control study
AU - Hearnden, Vanessa
AU - Murdoch, Craig
AU - D'Apice, Katy
AU - Duthie, Susan
AU - Hayward, Nicholas J
AU - Powers, Hilary Jane
N1 - © Author(s) (or their employer(s)) 2018. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
PY - 2018/11
Y1 - 2018/11
N2 - OBJECTIVES: This study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration.DESIGN: This was an observational study to determine oral HR-HPV prevalence in the study population. A case-control study was performed to explore the association between infection and folate status.SETTING: This study was conducted in Sheffield, UK, between April 2013 and August 2014.PARTICIPANTS: Seven hundred participants, aged 18-60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223).INTERVENTIONS: Participants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration.OUTCOME MEASURES: The prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection.RESULTS: The prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection.CONCLUSIONS: The prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity.TRIAL REGISTRATION NUMBER: ID14106.
AB - OBJECTIVES: This study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration.DESIGN: This was an observational study to determine oral HR-HPV prevalence in the study population. A case-control study was performed to explore the association between infection and folate status.SETTING: This study was conducted in Sheffield, UK, between April 2013 and August 2014.PARTICIPANTS: Seven hundred participants, aged 18-60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223).INTERVENTIONS: Participants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration.OUTCOME MEASURES: The prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection.RESULTS: The prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection.CONCLUSIONS: The prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity.TRIAL REGISTRATION NUMBER: ID14106.
U2 - 10.1136/bmjopen-2018-022497
DO - 10.1136/bmjopen-2018-022497
M3 - Article
VL - 8
JO - BMJ Open
JF - BMJ Open
SN - 2044-6055
IS - 8
M1 - e022497
ER -